| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 138,675 | T→C | 6.0% | N498D (AAT→GAT) ‡ | htrE ← | predicted outer membrane usher protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 138,675 | 0 | T | C | 6.0% | 354.3 / 11.1 | 118 | N498D (AAT→GAT) ‡ | htrE | predicted outer membrane usher protein |
| Reads supporting (aligned to +/- strand): ref base T (65/45); new base C (4/3); total (70/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 119 total aligned reads displayed.
CTTTTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATT > minE/138609‑138744 | cTTTTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACg > 1:630053/1‑70 (MQ=255) ttttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACg < 1:479278/67‑1 (MQ=255) ttttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATg > 1:1435694/1‑71 (MQ=255) tttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACg < 1:3165100/66‑1 (MQ=255) tttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATg > 1:4126721/1‑70 (MQ=255) tttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATg > 1:2420384/1‑70 (MQ=255) tttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATg > 1:1757488/1‑70 (MQ=255) ttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATgg < 1:1177864/70‑1 (MQ=255) ttCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATgg < 1:3049796/70‑1 (MQ=255) aaaTTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCg > 1:3719719/1‑71 (MQ=255) aaaTTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCg > 1:3105421/1‑71 (MQ=255) aaaTTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCg > 1:3094467/1‑71 (MQ=255) aaaTTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCg > 1:1004124/1‑71 (MQ=255) tttCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGtt > 1:3597348/1‑71 (MQ=255) tttCAACGGTTGGTTAATACTGACCGTAACCTGATATTTCATGCGTGAGTAATAACGCATgg > 1:2151492/1‑62 (MQ=255) cAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCa > 1:4112014/1‑56 (MQ=255) cAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCa < 1:1403787/70‑1 (MQ=255) aaCGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCaa < 1:2131423/70‑1 (MQ=255) gttggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATgg < 1:2166734/54‑1 (MQ=255) tggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTg > 1:1921383/1‑71 (MQ=255) tggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTg > 1:2954923/1‑71 (MQ=255) ggttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTg < 1:1868192/70‑1 (MQ=255) gttAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGtt < 1:526344/71‑1 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc > 1:155244/1‑63 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc > 1:279796/1‑63 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc > 1:383213/1‑63 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg > 1:2924489/1‑71 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg > 1:2334553/1‑71 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg > 1:1623906/1‑71 (MQ=255) aaTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCg < 1:713027/70‑1 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGAt > 1:3366235/1‑61 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGAt > 1:1416778/1‑61 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCggg < 1:1579855/71‑1 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg < 1:1549132/70‑1 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg < 1:2489645/70‑1 (MQ=255) aTACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg < 1:334817/70‑1 (MQ=255) aCTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc < 1:4298124/60‑1 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCaa > 1:4057896/1‑55 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATc > 1:548630/1‑59 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGAt < 1:2195673/70‑1 (MQ=255) cTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATg > 1:478779/1‑71 (MQ=255) gACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGtt > 1:1036854/1‑71 (MQ=255) aCCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGttt < 1:321674/71‑1 (MQ=255) aCCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGttt < 1:3120584/71‑1 (MQ=255) aCCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGttt < 1:3919660/71‑1 (MQ=255) gTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTc < 1:146049/47‑1 (MQ=255) tAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACt > 1:3627984/1‑71 (MQ=255) tAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACt > 1:2555248/1‑71 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGAt > 1:4023860/1‑61 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGAt > 1:1919919/1‑61 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGAt > 1:1147256/1‑61 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTc > 1:79171/1‑66 (MQ=255) aCCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACtt > 1:747287/1‑70 (MQ=255) gATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTtcatc > 1:4102601/1‑70 (MQ=255) tttttCATGCGTGAGTAATTACGCATGGATTTCGGTTCaa < 1:1570073/40‑1 (MQ=255) tttttCATGCGTGAGTAATTACGCATGGATTTCGGTTCaa < 1:1033615/40‑1 (MQ=255) tttttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTtcatc < 1:244486/68‑1 (MQ=255) tttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCtgg < 1:3618505/51‑1 (MQ=255) tttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAAtt < 1:1475779/70‑1 (MQ=255) atccATGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAAt > 1:616378/4‑69 (MQ=25) ttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTa < 1:2923398/70‑1 (MQ=255) ttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTa < 1:1417112/70‑1 (MQ=255) ttCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTa < 1:2648552/70‑1 (MQ=255) tCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCtt > 1:3215845/1‑42 (MQ=255) tCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCtt > 1:863713/1‑42 (MQ=255) tCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCtt > 1:1660399/1‑42 (MQ=255) tCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCtt > 1:2362988/1‑42 (MQ=255) tCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCtt > 1:1078763/1‑42 (MQ=255) ccATGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACtt < 1:4251833/59‑1 (MQ=25) ccATGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACtt < 1:406446/59‑1 (MQ=25) ccATGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACtt < 1:2730351/59‑1 (MQ=25) aTGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGt < 1:1069039/71‑1 (MQ=255) aTGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTtca > 1:1580655/1‑60 (MQ=37) aTGCGTAATTACTCACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTtca > 1:3247666/1‑60 (MQ=37) tGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGt > 1:65728/1‑70 (MQ=255) tGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTc < 1:783074/49‑1 (MQ=255) tGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTc < 1:105678/49‑1 (MQ=255) tGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTc < 1:1743654/49‑1 (MQ=255) tGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGt < 1:933718/66‑1 (MQ=255) aGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGtt > 1:1269698/1‑45 (MQ=255) aGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCAc > 1:3576342/1‑49 (MQ=255) aGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCAc > 1:4424158/1‑49 (MQ=255) aGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAg > 1:895935/1‑67 (MQ=255) aGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAg > 1:4249771/1‑67 (MQ=255) aGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAg > 1:1790881/1‑67 (MQ=255) aGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAg > 1:1865245/1‑67 (MQ=255) aGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAg > 1:2711126/1‑67 (MQ=255) gTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGcat > 1:4441266/1‑71 (MQ=255) gTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGcat > 1:2450160/1‑71 (MQ=255) gTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGcat > 1:3297332/1‑71 (MQ=255) tAATTACGCATGGATTTCGGTTCAAGATCTTGTTCgg < 1:2177230/37‑1 (MQ=255) aTTACGCATGGATTTCGGTTCAAGATCTTGTTCgg > 1:481588/1‑35 (MQ=255) aTTACGCATGGATTTCGGTTCAAGATCTTGTTCgg > 1:1118508/1‑35 (MQ=255) aTTACGCATGGATTTCGGTTCAAGATCTTGTTCgg > 1:3706993/1‑35 (MQ=255) aTTACGCATGGATTTCGGTTCAAGATCTTGTTCgg > 1:3524552/1‑35 (MQ=255) aTTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGcatcat > 1:2038196/1‑71 (MQ=255) aTTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGcatcat > 1:1255225/1‑71 (MQ=255) aTTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGcatcat > 1:2846594/1‑71 (MQ=255) aTTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGcatca < 1:3056988/70‑1 (MQ=255) tACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCAtt > 1:2665472/1‑70 (MQ=255) | CTTTTTTCTCAAATTTCAACGGTTGGTTAATACTGACCGTAACCTGATTTTTCATGCGTGAGTAATTACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATT > minE/138609‑138744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |