| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,892,019:1 | +GC | 100% | intergenic (‑437/+54) | gudP ← / ← ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,892,019 | 1 | . | G | 100.0% | 294.3 / NA | 81 | intergenic (‑437/+54) | gudP/ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (35/46); total (35/46) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 1,892,019 | 2 | . | C | 100.0% | 294.1 / NA | 81 | intergenic (‑437/+54) | gudP/ygdL | predicted D‑glucarate transporter/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (35/46); total (35/46) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACC > minE/1891950‑1892078 || aCTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCag > 1:661764/1‑70 (MQ=255)aCTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCag > 1:5688968/1‑70 (MQ=255) ggCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCagct > 1:4072981/1‑67 (MQ=255) gCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca < 1:960899/70‑1 (MQ=255) gCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca < 1:5289024/70‑1 (MQ=255) gCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca < 1:6559450/70‑1 (MQ=255) gCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca < 1:86682/70‑1 (MQ=255) ccTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa < 1:6797285/71‑1 (MQ=255) ccTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGa < 1:2029468/71‑1 (MQ=255) gAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc > 1:2808216/1‑71 (MQ=255) gAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAAcc > 1:4637470/1‑71 (MQ=255) aaaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCgg > 1:1373452/1‑71 (MQ=255) aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGCACATATCAGCTAAGCACAGAACCggg > 1:4334660/1‑71 (MQ=255) aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCggg > 1:4074516/1‑71 (MQ=255) aaGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCggg > 1:3952391/1‑71 (MQ=255) ttcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCgg < 1:1824342/71‑1 (MQ=255) ttcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCgg < 1:1656555/71‑1 (MQ=255) ttcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCg > 1:2076698/1‑70 (MQ=255) tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa > 1:4150668/1‑71 (MQ=255) tcgattcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGa > 1:6870979/1‑71 (MQ=255) attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa < 1:4087803/71‑1 (MQ=255) attcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa < 1:5629547/71‑1 (MQ=255) ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg < 1:1930921/71‑1 (MQ=255) ttcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAg < 1:6550277/71‑1 (MQ=255) tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca > 1:2585690/1‑53 (MQ=255) tcAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca > 1:4069495/1‑53 (MQ=255) cAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGAc > 1:5435070/1‑71 (MQ=255) gCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt < 1:6937199/71‑1 (MQ=255) cACCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGtt < 1:727298/71‑1 (MQ=255) aCCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt > 1:1961195/1‑69 (MQ=255) aCCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt > 1:35070/1‑69 (MQ=255) aCCGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTc < 1:3813722/71‑1 (MQ=255) cGCTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc > 1:4004821/1‑71 (MQ=255) cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa < 1:4401715/60‑1 (MQ=255) cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgcg > 1:439338/1‑70 (MQ=255) cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc < 1:4516998/69‑1 (MQ=255) cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc > 1:2405130/1‑69 (MQ=255) cTAAAACGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt < 1:6536207/71‑1 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa > 1:6089262/1‑60 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGa > 1:2891421/1‑60 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt < 1:5540081/63‑1 (MQ=255) aaaaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGt < 1:5811853/63‑1 (MQ=255) aaCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtctc < 1:2162215/70‑1 (MQ=255) aCGACATTTACCGCTCGCTGAACATATCAGCTAAGcaca < 1:89793/39‑1 (MQ=37) aCGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGGCGGATAAGACGTTCGCGTCTCAt < 1:5001115/71‑1 (MQ=255) cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa < 1:5237742/54‑1 (MQ=255) cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt < 1:292253/65‑1 (MQ=255) cGACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGt < 1:3365375/65‑1 (MQ=255) gACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATcc > 1:2413486/1‑71 (MQ=255) gACATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATcc < 1:1440788/71‑1 (MQ=255) cATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGa < 1:3736954/71‑1 (MQ=255) cATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGa < 1:4895188/71‑1 (MQ=255) cATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGa < 1:1220400/71‑1 (MQ=255) cATTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGa < 1:612437/71‑1 (MQ=255) aTTTACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACg < 1:3707406/54‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc < 1:5618986/58‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc < 1:4343810/58‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc < 1:1316926/58‑1 (MQ=255) tttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTcgc < 1:2584361/58‑1 (MQ=255) ttACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGGTaa > 1:1474205/1‑48 (MQ=255) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg > 1:5329794/1‑37 (MQ=25) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg > 1:6806219/1‑37 (MQ=25) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg > 1:3147051/1‑37 (MQ=25) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg > 1:3308435/1‑37 (MQ=25) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCg > 1:3923166/1‑37 (MQ=25) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa > 1:3845520/1‑47 (MQ=255) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa > 1:2856123/1‑47 (MQ=255) tACCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa > 1:666159/1‑47 (MQ=255) aCCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGtc > 1:1598530/1‑58 (MQ=255) aCCGCTCGCTGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTg < 1:731508/70‑1 (MQ=38) cgctcgctGAACATATCAGCTAAGCACAGAACCGGGTCg > 1:5042733/1‑39 (MQ=37) gctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTg < 1:653233/71‑1 (MQ=39) ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGAc > 1:5818742/1‑63 (MQ=38) ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGAc > 1:2170313/1‑63 (MQ=38) ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGa > 1:5100706/1‑67 (MQ=38) ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGt < 1:6649938/71‑1 (MQ=255) ctcgctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGt < 1:5508627/71‑1 (MQ=255) cgctGAACATATCAGCTAAGCACAGAACCGGGTCGGAt < 1:5119016/38‑1 (MQ=25) gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt < 1:5084469/70‑1 (MQ=38) gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt < 1:3177435/70‑1 (MQ=38) gctGAACATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGttt < 1:3909332/70‑1 (MQ=38) ctGAACATATCAGCTAAGCACAGAACCGGGTCGGATaa > 1:6546457/1‑38 (MQ=25) tGAACATATCAGCTAAGCACATAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTaa < 1:5683252/70‑1 (MQ=255) aaCATATCAGCTAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAAcc < 1:3997645/70‑1 (MQ=255) || ACTGGCATTTGCCTGAAAAGATTCGATTCAGCACCGCTAAAACGACATTTACCGCTCGCTGAACATATCA‑‑TAAGCACAGAACCGGGTCGGATAAGACGTTCGCGTCTCATCCGACCTGATTGTTTAACC > minE/1891950‑1892078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |