Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | minE | 214,925 | A→C | 56.9% | D12A (GAT→GCT) | dnaQ → | DNA polymerase III epsilon subunit |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | minE | 214,925 | 0 | A | C | 56.9% | 356.3 / inf | 315 | D12A (GAT→GCT) | dnaQ | DNA polymerase III epsilon subunit |
Reads supporting (aligned to +/- strand): ref base A (67/69); new base C (92/87); total (159/156) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.34e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 326 total aligned reads displayed.
GTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGC > minE/214859‑214994 | gTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATAcc > 1:2059331/1‑71 (MQ=255) tAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATAcc > 1:3219561/1‑70 (MQ=255) aaaCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGa > 1:6150938/1‑70 (MQ=255) aCGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac < 1:5998510/71‑1 (MQ=255) cAAGTCTGACATAAATGACCG‑‑ATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg > 1:6464617/1‑71 (MQ=255) gTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACAGCAACCACCgg > 1:2816281/1‑70 (MQ=255) tGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTAt > 1:5262300/1‑70 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGaa > 1:6243736/1‑71 (MQ=255) aCATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGa < 1:3623842/70‑1 (MQ=255) cATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAc > 1:6673372/1‑71 (MQ=255) aTAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGGAc < 1:3985830/70‑1 (MQ=255) tAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAAcc < 1:1288364/70‑1 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGa > 1:6376756/1‑45 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGa > 1:4181352/1‑45 (MQ=255) aaTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa < 1:1816423/71‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:4878024/71‑1 (MQ=255) aTGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAt < 1:5176730/71‑1 (MQ=255) cGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTgg < 1:2995022/69‑1 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCgca < 1:58431/44‑3 (MQ=255) cTATGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTg > 1:2421446/1‑69 (MQ=255) tATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:1693821/1‑45 (MQ=255) tATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAac > 1:353300/1‑45 (MQ=255) ttaGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc < 1:5316751/69‑1 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:2251587/61‑1 (MQ=255) tGAGCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTgcgc < 1:2596130/69‑1 (MQ=255) gAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCAc > 1:5524798/1‑70 (MQ=255) aGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAt > 1:4754058/1‑59 (MQ=255) gCACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGTACCa < 1:3514138/55‑1 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt > 1:4129109/1‑70 (MQ=255) cACTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt > 1:1424228/1‑70 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGa > 1:6728776/1‑55 (MQ=255) aCTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGa < 1:1449202/71‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:3581420/43‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCgg < 1:3379848/43‑1 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTa > 1:4302806/1‑67 (MQ=255) cTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGt > 1:4911808/1‑59 (MQ=255) tGCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:760162/68‑1 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2513759/67‑1 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:5626736/1‑71 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:6929653/1‑71 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:1172556/1‑71 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:6558965/1‑71 (MQ=255) gCAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAgg > 1:1578277/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGc > 1:5047376/1‑71 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGa > 1:1671603/1‑51 (MQ=255) cAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGaa > 1:4009000/1‑68 (MQ=255) aaTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt < 1:2280700/52‑1 (MQ=255) aaTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt < 1:4261479/52‑1 (MQ=255) aaTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt < 1:1870972/52‑1 (MQ=255) aaTTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGAtt < 1:3885035/52‑1 (MQ=255) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCgg < 1:3444969/38‑1 (MQ=255) aTTACACGCCAGATCGTTCTCGATACCGAAACCACCgg < 1:2577516/38‑1 (MQ=255) ttACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac < 1:2149608/71‑1 (MQ=255) ttACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCca > 1:4564754/1‑70 (MQ=255) tACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:5096151/62‑1 (MQ=255) tACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:1082158/62‑1 (MQ=255) tACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:1128819/62‑1 (MQ=255) tACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:2161899/62‑1 (MQ=255) tACACGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:3681086/62‑1 (MQ=255) tACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:2671495/1‑70 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTa > 1:4463372/1‑37 (MQ=37) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCAc > 1:3153739/1‑57 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:962961/1‑64 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAg > 1:6125378/1‑64 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:633670/1‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:4693590/1‑69 (MQ=255) acacGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:4019956/1‑69 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTAt > 1:4501811/1‑60 (MQ=255) acacGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCcac > 1:3820012/1‑69 (MQ=255) acGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAtt < 1:6860921/46‑1 (MQ=39) acGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGa < 1:884430/71‑1 (MQ=255) cGCCAGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGAt < 1:4398809/71‑1 (MQ=255) ccAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCa > 1:5298352/1‑71 (MQ=255) aGATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt < 1:3357286/71‑1 (MQ=255) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:5386154/52‑1 (MQ=255) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:5464595/52‑1 (MQ=255) gATCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:508269/52‑1 (MQ=255) aTCGTTCTCGCTACCGCAACCACCGGTATGAACCAGAt < 1:717095/38‑1 (MQ=37) aTCGTTCTCGATACCGAAACCACCGGTATGAACCa < 1:604195/35‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCa < 1:3709698/35‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCa < 1:4641915/35‑1 (MQ=255) aTCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTAt < 1:6827372/51‑1 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACt > 1:2750517/1‑48 (MQ=39) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACt > 1:4978049/1‑48 (MQ=39) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACt > 1:6408858/1‑48 (MQ=39) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACt > 1:1006947/1‑48 (MQ=39) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:271982/1‑68 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:5682331/1‑68 (MQ=255) tCGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:2022672/1‑68 (MQ=255) cGTTCTCGCTACCGCAACCACCTGTATGAACCAGATTGGTGCGCACTATg < 1:162799/50‑1 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:467303/1‑67 (MQ=255) cGTTCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCAtt > 1:6591577/1‑67 (MQ=255) cGTTCTCGATACCGAAACCACCGGTATGAACCAGa > 1:1748126/1‑35 (MQ=255) ttCTCGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGAtt < 1:2931095/71‑1 (MQ=255) ttCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAg < 1:1332545/59‑1 (MQ=255) ctcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTgg > 1:3392598/1‑71 (MQ=255) ctcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGa > 1:486156/1‑58 (MQ=255) ctcGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTg > 1:5964623/1‑70 (MQ=255) tcGCTACCGCAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGt > 1:2330421/1‑71 (MQ=255) aTACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATg < 1:1479543/42‑1 (MQ=255) | GTAAAACGACAAGTCTGACATAAATGACCGCTATGAGCACTGCAATTACACGCCAGATCGTTCTCGATACCGAAACCACCGGTATGAACCAGATTGGTGCGCACTATGAAGGCCACAAGATCATTGAGATTGGTGC > minE/214859‑214994 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |