| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 266,222 | C→T | 6.7% | R128H (CGC→CAC) | yajO ← | predicted oxidoreductase, NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 266,222 | 0 | C | T | 6.7% | 389.8 / 13.2 | 119 | R128H (CGC→CAC) | yajO | predicted oxidoreductase, NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base C (43/68); new base T (4/4); total (47/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
CTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAGCGCAAAAT > minE/266154‑266292 | cTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGa > 1:6331142/1‑71 (MQ=255) ccGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGaa < 1:5592786/70‑1 (MQ=255) ggCTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAAttt > 1:6719826/1‑71 (MQ=255) gCTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTg < 1:6856338/71‑1 (MQ=255) gCTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTg > 1:808377/1‑71 (MQ=255) gCTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTg < 1:1647494/71‑1 (MQ=255) cTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGc < 1:891099/71‑1 (MQ=255) cTTTTACCACGGCGTTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGc < 1:3893911/71‑1 (MQ=255) cgtTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACa > 1:1658992/1‑71 (MQ=255) tGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATaa > 1:3969176/1‑71 (MQ=255) tGAGGGCTTACAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATaa > 1:5619077/1‑71 (MQ=255) gAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAAt > 1:40502/1‑71 (MQ=255) aGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGTGATGAATTTGCAg < 1:1734293/56‑1 (MQ=255) aGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGTGATGAATTTGCAg < 1:2583789/56‑1 (MQ=255) aGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGTGATGAATTTGCAg < 1:3243187/56‑1 (MQ=255) aGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATc < 1:1194296/71‑1 (MQ=255) aGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATc < 1:2125492/71‑1 (MQ=255) ggCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGTGATGAATTTGCAGGa > 1:2886433/1‑56 (MQ=255) ggCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGTGATGAATTTGCAGGa > 1:5192186/1‑56 (MQ=255) ggCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGTGATGAATTTGCAGGa > 1:5791343/1‑56 (MQ=255) ggCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGTGATGAATTTGCAGGa > 1:3084132/1‑56 (MQ=255) cTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATg > 1:4301282/1‑71 (MQ=255) cTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATg > 1:5739823/1‑71 (MQ=255) tCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGata < 1:6880805/54‑1 (MQ=255) tCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGata < 1:1356945/54‑1 (MQ=255) tCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGata < 1:1553923/54‑1 (MQ=255) gCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACa < 1:3433177/56‑1 (MQ=255) gCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACa < 1:2568945/56‑1 (MQ=255) gCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACa < 1:3471654/56‑1 (MQ=255) gCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACa < 1:2686333/56‑1 (MQ=255) gCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACa < 1:5908534/56‑1 (MQ=255) gCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACa < 1:5776247/56‑1 (MQ=255) gCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCgaga > 1:89083/1‑71 (MQ=255) gCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCgaga > 1:171659/1‑71 (MQ=255) gTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATa > 1:65365/1‑56 (MQ=255) tctTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTc > 1:3234921/1‑70 (MQ=255) tctTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCg > 1:3885955/1‑71 (MQ=255) tctTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCg > 1:5474087/1‑71 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGc < 1:1087405/37‑1 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGc < 1:568198/37‑1 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATc < 1:2040860/54‑1 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATc < 1:1137104/54‑1 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATc < 1:1862470/54‑1 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATc < 1:3400287/54‑1 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATc < 1:5872736/54‑1 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTc < 1:584968/68‑1 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCg > 1:5728299/1‑69 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCg < 1:5618236/69‑1 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCg > 1:112233/1‑69 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCg > 1:271672/1‑69 (MQ=255) ttCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCg > 1:34773/1‑69 (MQ=255) cGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAg > 1:5956494/1‑37 (MQ=255) cGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAg > 1:6277705/1‑37 (MQ=255) cGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGATGTCGCAg > 1:3789245/1‑70 (MQ=255) cGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAgg > 1:6041524/1‑71 (MQ=255) cGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAg < 1:18269/70‑1 (MQ=255) gATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGAc < 1:4149629/45‑1 (MQ=255) tCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTg < 1:5121867/71‑1 (MQ=255) tCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTg < 1:289796/71‑1 (MQ=255) tCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTg < 1:3013901/71‑1 (MQ=255) tCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTg < 1:578190/71‑1 (MQ=255) tCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTg < 1:5456107/71‑1 (MQ=255) tCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTg < 1:2063199/71‑1 (MQ=255) tCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTg < 1:471971/71‑1 (MQ=255) tCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTg < 1:4679543/71‑1 (MQ=255) tCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTg < 1:1683172/71‑1 (MQ=255) ggCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCa > 1:5633215/1‑49 (MQ=255) ggCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCa > 1:4201592/1‑49 (MQ=255) tgttgtAATCCCAGCGATGAATTTGCATGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGtcgtcg < 1:4121255/71‑1 (MQ=255) tgttgtAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCt < 1:1830323/64‑1 (MQ=255) tgttgtAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCt < 1:3466762/64‑1 (MQ=255) tgttgtAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGtcgtcg < 1:1428232/71‑1 (MQ=255) tgttgtAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGtcgtcg < 1:2487657/71‑1 (MQ=255) tgttgtAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGtcgtcg < 1:338451/71‑1 (MQ=255) tgttgtAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGtcgtcg < 1:1380495/71‑1 (MQ=255) ttgtAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGAt < 1:5839881/71‑1 (MQ=255) tAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAg < 1:1161508/56‑1 (MQ=255) tAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAg < 1:3799484/56‑1 (MQ=255) tAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAg < 1:422616/56‑1 (MQ=255) tAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAg < 1:5289233/56‑1 (MQ=255) tAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAg < 1:4559369/56‑1 (MQ=255) tAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAg < 1:5064784/56‑1 (MQ=255) tAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATaga < 1:6453837/71‑1 (MQ=255) tAATCCAAGCGATGAATTTGCAGGATATCGACTTAATCCATGCCGAGACGTCGCAg < 1:346638/56‑1 (MQ=255) aTCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATagag < 1:1776/70‑1 (MQ=255) tCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTgtcgt < 1:4140216/61‑1 (MQ=255) tCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTgtcgt < 1:3252838/61‑1 (MQ=255) tCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTgtcgt < 1:5431607/61‑1 (MQ=255) ccAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAgcgc > 1:865556/1‑70 (MQ=255) ccAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAgcg > 1:4320725/1‑69 (MQ=255) cAGCGATGAATTTGCAGGATATCGACATAATCCATGCCg > 1:2277577/1‑39 (MQ=255) cAGCGATGAATTTGCAGGATATCGACATAATCCATGCCg > 1:684870/1‑39 (MQ=255) aGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAGCGCaa > 1:2586268/1‑70 (MQ=255) aGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAGCGCaa > 1:3099473/1‑70 (MQ=255) aGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAGCGCa > 1:5096742/1‑69 (MQ=255) gCGATGAATTTGCAGGATATCGACATAATCCATGACGAGACGTCGCAGGCTGTCGTCGATAGAGCGCaaaa > 1:5469000/1‑71 (MQ=255) cGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAGCGCAAAAt > 1:4669791/1‑71 (MQ=255) cGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAGCGCAAAAt > 1:64007/1‑71 (MQ=255) cGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAGCGCAAAAt > 1:6041929/1‑71 (MQ=255) cGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAGCGCAAAAt > 1:2219599/1‑71 (MQ=255) | CTTTCCCGGCTTTTACCACGTCGTTGAGGGCTTCCAGCGTCTCTTCGATCGGCGTGTTGTAATCCCAGCGATGAATTTGCAGGATATCGACATAATCCATGCCGAGACGTCGCAGGCTGTCGTCGATAGAGCGCAAAAT > minE/266154‑266292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |