| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,028,431 | C→T | 8.7% | A462A (GCC→GCT) | malX → | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,028,431 | 0 | C | T | 8.7% | 473.4 / 25.2 | 149 | A462A (GCC→GCT) | malX | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Reads supporting (aligned to +/- strand): ref base C (64/72); new base T (5/8); total (69/80) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 154 total aligned reads displayed.
AAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTA > minE/1028361‑1028501 | aaGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGcc < 1:4427491/71‑1 (MQ=255) ccGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGa > 1:4019434/1‑70 (MQ=255) cGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGTAGCATTAGGCGGTGCCGAc > 1:2029915/1‑70 (MQ=255) cGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGAc < 1:5987200/70‑1 (MQ=255) gTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACaa < 1:1335531/71‑1 (MQ=255) cGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGAc < 1:5874601/64‑1 (MQ=255) cGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATAtt < 1:6510940/70‑1 (MQ=255) cGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTg < 1:3820353/71‑1 (MQ=255) cgcCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGc > 1:3685251/1‑71 (MQ=255) cGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTc < 1:260361/71‑1 (MQ=255) gTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGAt < 1:2560371/71‑1 (MQ=255) gTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGAt < 1:5511302/71‑1 (MQ=255) tAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATa < 1:5377023/71‑1 (MQ=255) tCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCa < 1:540391/58‑1 (MQ=255) cAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCa < 1:5461682/57‑1 (MQ=255) cAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTg > 1:4319881/1‑70 (MQ=255) aGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGt > 1:6270877/1‑54 (MQ=255) aGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCa > 1:5732304/1‑71 (MQ=255) aGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCa > 1:4355116/1‑71 (MQ=255) aGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCa > 1:4140490/1‑71 (MQ=255) aGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCa > 1:6835701/1‑71 (MQ=255) ggTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGAt < 1:2914532/63‑1 (MQ=255) gTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCTGACAATATTGTcg < 1:5853152/54‑2 (MQ=255) gTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCTGACAATATTGTcg < 1:6406630/54‑2 (MQ=255) gTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCAt > 1:4803458/1‑70 (MQ=255) gTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCAt > 1:3113305/1‑70 (MQ=255) gTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCAt > 1:6684698/1‑70 (MQ=255) ttACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTa > 1:433672/1‑71 (MQ=255) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTTCTGACAATATTGTCGGc > 1:5165247/1‑54 (MQ=38) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCTGACAATATTGTCGGc > 1:2353696/1‑54 (MQ=38) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCTGACAATATTGTCGGc > 1:3216399/1‑54 (MQ=38) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTg < 1:6227677/49‑1 (MQ=255) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTg < 1:749109/49‑1 (MQ=255) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTg < 1:1215072/49‑1 (MQ=255) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTg < 1:6060288/49‑1 (MQ=255) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTg < 1:905259/49‑1 (MQ=255) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTg < 1:927941/49‑1 (MQ=255) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTAc > 1:1855641/1‑71 (MQ=255) tACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTAc > 1:4617914/1‑71 (MQ=255) aCAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTAc < 1:3008345/70‑1 (MQ=255) cAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTAccc < 1:6429878/71‑1 (MQ=255) cAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTAccc < 1:35826/71‑1 (MQ=255) cAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTAccc < 1:1543135/71‑1 (MQ=255) aaCGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCg > 1:4613200/1‑71 (MQ=255) aaCGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCg > 1:947537/1‑71 (MQ=255) aaCGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCg < 1:1686350/71‑1 (MQ=255) aaCGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCg < 1:3707705/71‑1 (MQ=255) cGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTc > 1:4230145/1‑71 (MQ=255) cGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTc > 1:3325763/1‑71 (MQ=255) ttCCTGCAATCCTCGAAGCATTAGGCGGTGCTGACAATATTGTcg < 1:5878059/45‑2 (MQ=255) ttCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTa < 1:4020323/63‑1 (MQ=255) tCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTg > 1:6661526/1‑70 (MQ=255) tCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGc > 1:6411985/1‑71 (MQ=255) tCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGc > 1:1737459/1‑71 (MQ=255) ccTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCg > 1:5728817/1‑71 (MQ=255) cTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGt < 1:1387410/71‑1 (MQ=255) tGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGc < 1:6830783/43‑1 (MQ=255) tGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTc < 1:6770652/46‑1 (MQ=255) tGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTc < 1:3437834/46‑1 (MQ=255) tGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTc < 1:4287221/46‑1 (MQ=255) tGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTc < 1:2743247/46‑1 (MQ=255) tGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCAt < 1:3448304/57‑1 (MQ=255) cAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGttt < 1:2664668/70‑1 (MQ=255) cAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGtt > 1:2187764/1‑69 (MQ=255) cAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTg > 1:1137175/1‑71 (MQ=255) cAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTg > 1:1986012/1‑71 (MQ=255) cTCGAATCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCtgt > 1:6463831/1‑71 (MQ=255) cTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTc > 1:3922164/1‑58 (MQ=255) tCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGAt < 1:6223749/41‑1 (MQ=255) cGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCtgtg > 1:3036638/1‑70 (MQ=255) gAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGc > 1:3595056/1‑45 (MQ=255) gAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGc > 1:4170024/1‑45 (MQ=255) gAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGc > 1:1210797/1‑45 (MQ=255) gAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGc > 1:4358631/1‑45 (MQ=255) gAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGc > 1:1482563/1‑45 (MQ=255) gAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCtgtg > 1:6958982/1‑69 (MQ=255) aGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATaa > 1:211335/1‑39 (MQ=255) aGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATaa > 1:4463063/1‑39 (MQ=255) gCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGa < 1:3117986/71‑1 (MQ=255) cATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGat < 1:5428986/71‑1 (MQ=255) ttAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGaaa < 1:6960505/66‑1 (MQ=255) ttAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAg < 1:3217399/67‑1 (MQ=255) tAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGa < 1:2485902/67‑1 (MQ=255) aGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGa < 1:38725/66‑1 (MQ=255) ggCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTc < 1:5207011/71‑1 (MQ=255) ggCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTc < 1:4997951/71‑1 (MQ=255) gCGGTGCTGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCg < 1:5757540/71‑1 (MQ=255) gCGGTGCTGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCg < 1:3318034/71‑1 (MQ=255) gCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCg < 1:1872745/49‑1 (MQ=255) gCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCg < 1:72436/49‑1 (MQ=255) gCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCg < 1:793033/49‑1 (MQ=255) gCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCg < 1:801154/49‑1 (MQ=255) ggTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGtt > 1:2227463/1‑49 (MQ=255) ggTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCt < 1:2281271/71‑1 (MQ=255) gCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGt > 1:6405182/1‑71 (MQ=255) gCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGt > 1:6310455/1‑71 (MQ=255) gCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGt > 1:6030500/1‑71 (MQ=255) gCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGt > 1:1079371/1‑71 (MQ=255) ccGACAATATTGTCAGCATCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGt > 1:1936904/1‑70 (MQ=255) cGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTa < 1:4125647/71‑1 (MQ=255) | AAGCCGTTGCCGGTGCGCCGGGTAAATCAGGTTACAACGTTCCTGCAATCCTCGAAGCATTAGGCGGTGCCGACAATATTGTCAGCCTCGATAACTGCATTACCCGTCTGCGTTTGTCTGTGAAAGATATGTCGCTTGTTA > minE/1028361‑1028501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |