| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,363,919 | G→T | 5.4% | R470L (CGC→CTC) | metG → | methionyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,363,919 | 0 | G | T | 5.4% | 397.5 / 4.7 | 110 | R470L (CGC→CTC) | metG | methionyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (42/62); new base T (3/3); total (45/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
Only 100 of 120 total aligned reads displayed.
ATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTGAA > minE/1363859‑1363988 | aTCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCg < 1:4642032/69‑1 (MQ=255)aTCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGAc > 1:2282891/1‑71 (MQ=255)aTCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGGGGTGGCGAAACAGGAAGGCCGCGATGCCg < 1:3753177/69‑1 (MQ=255) tCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTTGCGAAACAGGAAGGCCTCGATGc < 1:4342911/66‑1 (MQ=255) tCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCTCGATGc < 1:1548884/66‑1 (MQ=255) tCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGCGGTGGCGAAACAGGAAGGCCTCGATGc < 1:6609745/66‑1 (MQ=255) cTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCt > 1:4169412/1‑71 (MQ=255) cTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCt > 1:1229594/1‑71 (MQ=255) cTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCt > 1:1099362/1‑71 (MQ=255) tGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCTCGATGCCg > 1:1357159/1‑66 (MQ=255) tGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCTCGATGCCg > 1:6674953/1‑66 (MQ=255) tGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCTCGATGCCg > 1:2293290/1‑66 (MQ=255) tGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTg > 1:3103076/1‑71 (MQ=255) tGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTg > 1:2733783/1‑71 (MQ=255) ggCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTg > 1:2099002/1‑70 (MQ=255) ggCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTg > 1:3909191/1‑70 (MQ=255) aCCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGc > 1:5972053/1‑70 (MQ=255) ccGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCaa < 1:409043/71‑1 (MQ=255) cTATGTCGATGAACAGGCTCCGTGGGTGGTCGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAAttt > 1:84984/1‑71 (MQ=255) tGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCt < 1:1515430/71‑1 (MQ=255) tGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCt < 1:3239405/71‑1 (MQ=255) tCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTc < 1:3164620/70‑1 (MQ=255) tCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTc < 1:6854499/70‑1 (MQ=255) tCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTc > 1:313249/1‑70 (MQ=255) tCGATGAACAGGCTCCGTGGGGGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTc < 1:5134961/70‑1 (MQ=255) cGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAAtt < 1:4705737/64‑1 (MQ=255) aTGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTg > 1:3924789/1‑64 (MQ=255) aTGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTg > 1:4923456/1‑64 (MQ=255) aTGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTg > 1:4484421/1‑64 (MQ=255) tGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCg < 1:3424296/47‑1 (MQ=255) gAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCcgc < 1:746914/39‑1 (MQ=255) gAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCcgc < 1:4059806/39‑1 (MQ=255) aaCAGGCTCCGTGGGTTGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATggg < 1:3533705/70‑1 (MQ=255) aaCAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCcgc < 1:6226786/38‑1 (MQ=255) aaCAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTg < 1:6826140/50‑1 (MQ=255) aaCAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTg < 1:331466/50‑1 (MQ=255) aaCAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTg < 1:5787537/50‑1 (MQ=255) aaCAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTg < 1:1876014/50‑1 (MQ=255) aaCAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTg < 1:1310512/50‑1 (MQ=255) aaCAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGc < 1:5115118/62‑1 (MQ=255) aaCAGGCTCCGTGGGT‑GTGGCGAAACAGGAAGGCcgc < 1:849892/37‑1 (MQ=38) aCAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCa < 1:5486667/71‑1 (MQ=255) aGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATc < 1:4045230/71‑1 (MQ=255) ggCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCATTGGGCATCa < 1:2976486/71‑1 (MQ=255) ggCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCa < 1:1769127/71‑1 (MQ=255) ggCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCa < 1:2809152/71‑1 (MQ=255) ggCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCa < 1:5920486/71‑1 (MQ=255) gCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAAtt > 1:4861234/1‑54 (MQ=255) gCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCaa > 1:5924906/1‑71 (MQ=255) gCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCaa > 1:2389566/1‑71 (MQ=255) cTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAAttt > 1:6658628/1‑54 (MQ=255) cTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCaa > 1:6090120/1‑60 (MQ=255) cTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCaa > 1:3631613/1‑60 (MQ=255) cTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAAc < 1:83289/71‑1 (MQ=255) cGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTg < 1:2565607/71‑1 (MQ=255) cGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTg > 1:109033/1‑71 (MQ=255) cGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTg > 1:5771049/1‑71 (MQ=255) cGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTg > 1:3332483/1‑71 (MQ=255) gtgggtggTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGt > 1:3173528/1‑71 (MQ=255) gtgggtggTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGt > 1:1253436/1‑71 (MQ=255) gtgggtggTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGt > 1:3419766/1‑71 (MQ=255) gtgggtggTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGt > 1:1067080/1‑71 (MQ=255) gtggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCa < 1:1321418/39‑1 (MQ=255) gtggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCcgcg < 1:4315501/70‑1 (MQ=255) gtggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCcgcg < 1:4323818/70‑1 (MQ=255) tggCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAAc < 1:5984162/59‑1 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGCCCTGCAGGCAATTTGCTCaa > 1:4749336/1‑46 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTc < 1:6708026/44‑1 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTc < 1:4782505/44‑1 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTc < 1:3227775/44‑1 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCa < 1:1928121/52‑1 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCa < 1:3013897/52‑1 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCa < 1:3139317/52‑1 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCa < 1:6925151/52‑1 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCt < 1:1679635/71‑1 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCt < 1:6451684/71‑1 (MQ=255) gCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCt < 1:4333006/71‑1 (MQ=255) cGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCtg < 1:6679601/71‑1 (MQ=255) cGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCtg < 1:6786485/71‑1 (MQ=255) cGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCtg < 1:6220470/71‑1 (MQ=255) cGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCtg < 1:4976730/71‑1 (MQ=255) gAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAAc > 1:5358373/1‑55 (MQ=255) aaaCAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCa < 1:3828329/49‑1 (MQ=255) aaaCAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCtgat < 1:6053308/71‑1 (MQ=255) aaaCAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCtgat < 1:4900153/71‑1 (MQ=255) aaCAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCa < 1:6012660/48‑1 (MQ=255) aaCAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCtgat > 1:1010677/1‑70 (MQ=255) ggAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACt > 1:1050741/1‑70 (MQ=255) ggAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACt > 1:495041/1‑70 (MQ=255) ggCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCt > 1:4127299/1‑71 (MQ=255) ccGCGATGCGGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTg > 1:5146366/1‑70 (MQ=255) ccGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCt > 1:6202162/1‑69 (MQ=255) ccGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTg > 1:3510626/1‑70 (MQ=255) ccGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTg > 1:6228269/1‑70 (MQ=255) cgcgATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTGaa < 1:3384892/71‑1 (MQ=255) cgcgATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTGaa < 1:3810464/71‑1 (MQ=255) cgcgATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTGaa < 1:6915752/71‑1 (MQ=255) cgcgATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTGaa < 1:4796493/71‑1 (MQ=255) cgcgATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTGaa < 1:847118/71‑1 (MQ=255) cgcgATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTGaa < 1:5100567/71‑1 (MQ=255) | ATCTGGCTAACCGCTATGTCGATGAACAGGCTCCGTGGGTGGTGGCGAAACAGGAAGGCCGCGATGCCGACCTGCAGGCAATTTGCTCAATGGGCATCAACCTGTTCCGCGTGCTGATGACTTACCTGAA > minE/1363859‑1363988 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |