| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,364,064 | A→G | 5.9% | Q518Q (CAA→CAG) | metG → | methionyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,364,064 | 0 | A | G | 5.9% | 397.0 / 13.2 | 136 | Q518Q (CAA→CAG) | metG | methionyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (75/53); new base G (2/6); total (77/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.71e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.90e-01 | |||||||||||
Only 100 of 141 total aligned reads displayed.
CTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAG > minE/1363996‑1364134 | cTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAcc > 1:6880522/1‑71 (MQ=255)cTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAcc < 1:435430/71‑1 (MQ=255) gCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgc > 1:4615273/1‑71 (MQ=255) gCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgc > 1:6782620/1‑71 (MQ=255) ccGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAcc < 1:1320214/68‑1 (MQ=255) ccGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCg > 1:4846425/1‑69 (MQ=255) ccGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCg > 1:3938602/1‑69 (MQ=255) ccGAAACTGACAGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAcc > 1:662759/1‑68 (MQ=255) gAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAc > 1:4584962/1‑65 (MQ=255) gAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAc > 1:5456098/1‑65 (MQ=255) gAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc > 1:2498604/1‑71 (MQ=255) aaaCTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgct < 1:745420/71‑1 (MQ=255) aCTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg < 1:2185706/71‑1 (MQ=255) aCTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg < 1:2177615/71‑1 (MQ=255) tGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgct < 1:437496/64‑1 (MQ=255) tGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgct < 1:5292395/64‑1 (MQ=255) aCCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgct > 1:5038352/1‑62 (MQ=255) aCCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgct > 1:1830543/1‑62 (MQ=255) aCCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgct > 1:180676/1‑62 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATTGTATCCAGCAGCGGTTGCTgga < 1:2954472/66‑2 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAGCGGTTGCTgga < 1:5161809/66‑2 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAGCGGTTGCTgga < 1:5164954/66‑2 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAGCGGTTGCTgga < 1:3344061/66‑2 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAGCGGTTGCTgga < 1:1558256/66‑2 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAGCGGTTGCTgga < 1:5954260/66‑2 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc > 1:3281449/1‑62 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc > 1:2350668/1‑62 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc > 1:1287772/1‑62 (MQ=255) aGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAGCGGTTGCTggata > 1:359611/1‑63 (MQ=38) gCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCcaca > 1:2511542/1‑68 (MQ=255) gCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCcaca > 1:1966200/1‑68 (MQ=255) gCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGaa > 1:1886727/1‑71 (MQ=255) gCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGaa > 1:5653434/1‑71 (MQ=255) cAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAAt > 1:3005562/1‑71 (MQ=255) cAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAAt > 1:6243965/1‑71 (MQ=255) aGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATc < 1:3167302/71‑1 (MQ=255) aaGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc < 1:815278/51‑1 (MQ=255) aaGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc < 1:5576845/51‑1 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGctgctg > 1:2872828/1‑52 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGctgctg > 1:3309011/1‑52 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGctgctg > 1:6597671/1‑52 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGt < 1:69014/71‑1 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGt < 1:639436/71‑1 (MQ=255) gCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGtt > 1:854360/1‑71 (MQ=255) cATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg < 1:4434939/51‑1 (MQ=255) aTTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTc < 1:1415557/70‑1 (MQ=255) ttCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg < 1:2148937/49‑1 (MQ=255) ttCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCaa < 1:6751862/71‑1 (MQ=255) ttCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCaa < 1:5024511/71‑1 (MQ=255) ttCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCa < 1:1664902/70‑1 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAg > 1:2751322/1‑69 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc < 1:1230621/71‑1 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc < 1:3671504/71‑1 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc > 1:3705669/1‑71 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc < 1:2885535/71‑1 (MQ=255) tCAATACGGAACTGACCTGGGATTGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCaa < 1:580436/67‑1 (MQ=255) aaTACGGAACTGACCTGTGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgcg < 1:6486104/69‑1 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg > 1:3878542/1‑43 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg > 1:5854057/1‑43 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg > 1:2760341/1‑43 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg > 1:4883182/1‑52 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg > 1:6252204/1‑52 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg > 1:2818423/1‑52 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg > 1:107104/1‑52 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg > 1:2118160/1‑52 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGaa > 1:1687041/1‑56 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCt > 1:640323/1‑71 (MQ=255) tACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg < 1:1857539/50‑1 (MQ=255) cGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTg > 1:5181524/1‑50 (MQ=255) cGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTg > 1:5315720/1‑50 (MQ=255) cGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCaa > 1:6221822/1‑61 (MQ=255) cGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCCTTCaa > 1:1776526/1‑61 (MQ=255) ggAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGtata < 1:393070/71‑1 (MQ=255) ggAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGta < 1:2339270/69‑1 (MQ=255) tGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCg > 1:1565712/1‑70 (MQ=255) cTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCg < 1:638290/71‑1 (MQ=255) cTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCg < 1:6348871/71‑1 (MQ=255) tGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc < 1:5694735/53‑1 (MQ=255) ggATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATcc > 1:1648669/1‑42 (MQ=255) ggATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCg > 1:3489168/1‑43 (MQ=255) tGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGtata < 1:3636311/56‑1 (MQ=255) tGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGtata < 1:5745906/56‑1 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCa < 1:5021669/42‑1 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTg < 1:2502629/50‑1 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:3934044/1‑71 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:3169834/1‑71 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:3178017/1‑71 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg < 1:6454273/71‑1 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:2016702/1‑71 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg < 1:1829715/71‑1 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:3818542/1‑71 (MQ=255) aTCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGta > 1:4990390/1‑50 (MQ=255) tCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGatat < 1:189290/64‑1 (MQ=255) tCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGatat < 1:1155611/64‑1 (MQ=255) cAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGc > 1:6433746/1‑54 (MQ=255) cAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGa > 1:3628075/1‑64 (MQ=255) cAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAgg < 1:5426217/70‑1 (MQ=255) aaCCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTg > 1:6570698/1‑69 (MQ=255) aaCCGCTGCTGGGCAACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTg > 1:5260311/1‑69 (MQ=255) aCCGCTGCTGGGCCACAAAGTGAATCCGTTCAATGCGCTGTATAACCGCATCGATATGAGGCAGGtt < 1:2114566/67‑1 (MQ=255) | CTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAG > minE/1363996‑1364134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |