| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,364,066 | C→G | 5.9% | P519R (CCG→CGG) | metG → | methionyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,364,066 | 0 | C | G | 5.9% | 445.8 / 13.2 | 135 | P519R (CCG→CGG) | metG | methionyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (75/52); new base G (2/6); total (77/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.43e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.87e-01 | |||||||||||
Only 100 of 147 total aligned reads displayed.
CTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCA > minE/1363996‑1364136 | cTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAcc < 1:435430/71‑1 (MQ=255)cTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAcc > 1:6880522/1‑71 (MQ=255) gCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgc > 1:6782620/1‑71 (MQ=255) gCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgc > 1:6633848/1‑71 (MQ=255) ccGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAcc < 1:1320214/68‑1 (MQ=255) ccGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCg > 1:4846425/1‑69 (MQ=255) ccGAAACTGACAGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAAcc > 1:662759/1‑68 (MQ=255) gAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc > 1:2498604/1‑71 (MQ=255) aaaCTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgct < 1:745420/71‑1 (MQ=255) aCTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg < 1:2177615/71‑1 (MQ=255) tGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgct < 1:5292395/64‑1 (MQ=255) aCCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc > 1:5745740/1‑64 (MQ=255) aCCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgct > 1:180676/1‑62 (MQ=255) aCCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgct > 1:1830543/1‑62 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATTGTATCCAGCAGCGGTTGCTgga < 1:2954472/66‑2 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAGCGGTTGCTgga < 1:3344061/66‑2 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAGCGGTTGCTgga < 1:5954260/66‑2 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAGCGGTTGCTgga < 1:5161809/66‑2 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc > 1:1287772/1‑62 (MQ=255) cGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc > 1:3281449/1‑62 (MQ=255) gAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACaa < 1:2377530/71‑1 (MQ=255) gCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCcaca > 1:5163059/1‑68 (MQ=255) gCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCcaca > 1:2511542/1‑68 (MQ=255) gCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCcaca > 1:1966200/1‑68 (MQ=255) gCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGaa > 1:4188179/1‑71 (MQ=255) gCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGaa > 1:1886727/1‑71 (MQ=255) gCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGaa > 1:5653434/1‑71 (MQ=255) cAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAAt > 1:6243965/1‑71 (MQ=255) cAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAAt > 1:3005562/1‑71 (MQ=255) aGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATc < 1:3167302/71‑1 (MQ=255) aaGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctgc < 1:815278/51‑1 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctg > 1:4527632/1‑49 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCgctg > 1:4926717/1‑49 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGctgctg > 1:2297218/1‑52 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGctgctg > 1:2872828/1‑52 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGt < 1:69014/71‑1 (MQ=255) aGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGt < 1:639436/71‑1 (MQ=255) gCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGtt > 1:854360/1‑71 (MQ=255) cATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg < 1:4434939/51‑1 (MQ=255) aTTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTc < 1:1415557/70‑1 (MQ=255) ttCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg < 1:2148937/49‑1 (MQ=255) ttCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCaa < 1:5024511/71‑1 (MQ=255) ttCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCaa < 1:3933239/71‑1 (MQ=255) ttCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCa < 1:1664902/70‑1 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAg > 1:2751322/1‑69 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc < 1:3234397/71‑1 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc > 1:3705669/1‑71 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc < 1:3671504/71‑1 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc < 1:2885535/71‑1 (MQ=255) cTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc < 1:1230621/71‑1 (MQ=255) tCAATACGGAACTGACCTGGGATTGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCaa < 1:580436/67‑1 (MQ=255) tCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgc < 1:562063/70‑1 (MQ=255) aaTACGGAACTGACCTGTGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGgcg < 1:6486104/69‑1 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg > 1:5854057/1‑43 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg > 1:2760341/1‑43 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg > 1:3335900/1‑43 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTgg > 1:3878542/1‑43 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACaaa > 1:1584627/1‑51 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg > 1:2118160/1‑52 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg > 1:6252204/1‑52 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg > 1:107104/1‑52 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg > 1:2818423/1‑52 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGa > 1:3490105/1‑55 (MQ=255) aaTACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCt > 1:640323/1‑71 (MQ=255) tACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAg < 1:1857539/50‑1 (MQ=255) cGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTg > 1:5181524/1‑50 (MQ=255) cGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTg > 1:5315720/1‑50 (MQ=255) cGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCaa > 1:6221822/1‑61 (MQ=255) cGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCaa > 1:658690/1‑61 (MQ=255) cGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCCTTCaa > 1:1776526/1‑61 (MQ=255) ggAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGta < 1:2339270/69‑1 (MQ=255) tGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCg > 1:1565712/1‑70 (MQ=255) cTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCg < 1:6348871/71‑1 (MQ=255) cTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCg < 1:638290/71‑1 (MQ=255) cctgATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCg < 1:1686285/67‑1 (MQ=255) tGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGtata < 1:5745906/56‑1 (MQ=255) tGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGtata < 1:3636311/56‑1 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:3818542/1‑71 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg < 1:6454273/71‑1 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg < 1:1829715/71‑1 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:5030914/1‑71 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:4783593/1‑71 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:3934044/1‑71 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:2016702/1‑71 (MQ=255) gTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAgg > 1:3169834/1‑71 (MQ=255) aTCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGta > 1:4990390/1‑50 (MQ=255) tCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGatat < 1:1155611/64‑1 (MQ=255) tCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGatat < 1:189290/64‑1 (MQ=255) cAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGc > 1:6433746/1‑54 (MQ=255) cAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGa > 1:3628075/1‑64 (MQ=255) cAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAgg < 1:5426217/70‑1 (MQ=255) aaCCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTg > 1:6570698/1‑69 (MQ=255) aaCCGCTGCTGGGCAACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTg > 1:5260311/1‑69 (MQ=255) aCCGCTGCTGGGCCACAAAGTGAATCCGTTCAATGCGCTGTATAACCGCATCGATATGAGGCAGGtt < 1:2114566/67‑1 (MQ=255) ccGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGtt < 1:2659405/66‑1 (MQ=255) ccGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGc < 1:5507112/71‑1 (MQ=255) cGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCa < 1:3509659/71‑1 (MQ=255) cGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCa > 1:3765201/1‑71 (MQ=255) cGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCa < 1:524665/71‑1 (MQ=255) cGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCa < 1:1408560/71‑1 (MQ=255) | CTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGCCACAAAGTGAATCCGTTCAAGGCGCTGTATAACCGCATCGATATGAGGCAGGTTGAAGCA > minE/1363996‑1364136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |