BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	minE	1,814,958	A→G	5.1%	G73G (GGT→GGC)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,814,958	0	A	G	5.1%	420.7 / 10.2	138	G73G (GGT→GGC)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base A (71/60); new base G (4/3); total (75/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 166 total aligned reads displayed.

GTTGCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAA  >  minE/1814889‑1815026

 ttGCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTac                                                                     >  1:6561951/1‑70 (MQ=255)

      cAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcg                                                               >  1:2812308/1‑71 (MQ=255)

        gCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                             >  1:1049320/1‑71 (MQ=255)

            ttCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      <  1:5256640/58‑1 (MQ=255)

             tCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcac                                                        >  1:4279085/1‑71 (MQ=255)

             tCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcac                                                        >  1:3626822/1‑71 (MQ=255)

             tCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcac                                                        >  1:2486533/1‑71 (MQ=255)

               gaagaaGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcacgc                                                      <  1:6704483/71‑1 (MQ=255)

                 agaagGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTaccac                                                                  >  1:2273620/1‑57 (MQ=255)

                 agaagGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTac                                                                     <  1:118636/54‑1 (MQ=255)

                 agaagGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcacgca                                                     <  1:2968682/70‑1 (MQ=255)

                    agGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      >  1:5492963/1‑50 (MQ=255)

                    agGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      >  1:5372482/1‑50 (MQ=255)

                    agGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      >  1:6307604/1‑50 (MQ=255)

                    agGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      >  1:3630692/1‑50 (MQ=255)

                    agGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      >  1:3252502/1‑50 (MQ=255)

                    agGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:4963637/1‑71 (MQ=255)

                    agGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTCTGTTTa                                                                      >  1:6670574/1‑50 (MQ=255)

                       tATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTgg                                              <  1:2610213/71‑1 (MQ=255)

                         tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTaccac                                                                  <  1:2011103/49‑1 (MQ=255)

                         tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      <  1:3706259/45‑1 (MQ=255)

                         tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      <  1:549844/45‑1 (MQ=255)

                         tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      <  1:6854276/45‑1 (MQ=255)

                         tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      <  1:4965194/45‑1 (MQ=255)

                         tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTa                                                                      <  1:3230500/45‑1 (MQ=255)

                         tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:2460648/70‑1 (MQ=255)

                         tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:1708652/70‑1 (MQ=255)

                          ggTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTaccac                                                                  <  1:6100423/48‑1 (MQ=255)

                          ggTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTaccac                                                                  <  1:3315160/48‑1 (MQ=255)

                           gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTacc                                                                    >  1:5167648/1‑45 (MQ=255)

                           gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCAccc                                                                >  1:3751785/1‑49 (MQ=255)

                           gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgc                                                          <  1:4536589/55‑1 (MQ=255)

                           gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGaa                                           <  1:4904720/70‑1 (MQ=255)

                            tGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGt                                         >  1:6927302/1‑71 (MQ=255)

                               cgcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGt                                      <  1:6796990/71‑1 (MQ=255)

                               cgcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGt                                      <  1:2611455/71‑1 (MQ=255)

                                  gcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAgc                                   <  1:762287/71‑1 (MQ=255)

                                    agtgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGc                                                   <  1:693214/52‑1 (MQ=255)

                                    agtgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGc                                                   <  1:4039606/52‑1 (MQ=255)

                                     gtgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGcgcg                                <  1:3153994/71‑1 (MQ=255)

                                     gtgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGcgcg                                >  1:2035440/1‑71 (MQ=255)

                                     gtgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACACGCACGCACGCAGCGTTGGGAAGTGGTAGcgcg                                >  1:3290043/1‑71 (MQ=255)

                                      tgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGCgc                                                 >  1:5933786/1‑52 (MQ=255)

                                      tgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGCg                                                  >  1:4721570/1‑52 (MQ=255)

                                      tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:5084803/53‑1 (MQ=255)

                                      tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:772218/53‑1 (MQ=255)

                                      tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:5104585/53‑1 (MQ=255)

                                      tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:2434677/53‑1 (MQ=255)

                                      tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:3584305/53‑1 (MQ=255)

                                      tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:1440566/53‑1 (MQ=255)

                                      tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGcgcg                                <  1:1092097/70‑1 (MQ=255)

                                       gtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                             >  1:794459/1‑40 (MQ=255)

                                       gtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                             >  1:5368208/1‑40 (MQ=255)

                                       gtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCgg                               >  1:1956590/1‑70 (MQ=255)

                                        tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:6745771/55‑1 (MQ=255)

                                        tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCggg                              >  1:6191533/1‑70 (MQ=255)

                                        tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCggg                              >  1:3939106/1‑70 (MQ=255)

                                          aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:5267482/1‑49 (MQ=255)

                                          aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:6901468/1‑49 (MQ=255)

                                          aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:5966846/1‑49 (MQ=255)

                                          aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:6525830/1‑49 (MQ=255)

                                          aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:1404932/1‑49 (MQ=255)

                                          aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           <  1:3458024/71‑1 (MQ=255)

                                          aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            <  1:5396176/70‑1 (MQ=255)

                                          aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            >  1:2317881/1‑70 (MQ=255)

                                          aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            <  1:3151176/70‑1 (MQ=255)

                                           ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                          <  1:5786401/71‑1 (MQ=255)

                                           ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                          <  1:2751974/71‑1 (MQ=255)

                                           ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                          <  1:1030993/71‑1 (MQ=255)

                                                gctttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           <  1:4376835/63‑1 (MQ=255)

                                                 ttttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           <  1:4441685/64‑1 (MQ=255)

                                                 ttttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACg                    <  1:2849518/71‑1 (MQ=255)

                                                 ttttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACg                    <  1:6309461/71‑1 (MQ=255)

                                                   ttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCt                  >  1:5008827/1‑71 (MQ=255)

                                                     ccAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCtt                 <  1:2747321/70‑1 (MQ=255)

                                                      cAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTg                <  1:3861902/70‑1 (MQ=255)

                                                      cAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGt               <  1:4984460/71‑1 (MQ=255)

                                                        ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGTGAAGTGGTAGCGCGGGAATAATTACGCTTGTCg             <  1:6218943/71‑1 (MQ=255)

                                                        ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:5320192/1‑70 (MQ=255)

                                                        ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCg             <  1:6474001/71‑1 (MQ=255)

                                                        ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCg             <  1:948317/71‑1 (MQ=255)

                                                        ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCg             <  1:2089643/71‑1 (MQ=255)

                                                         gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:1663899/1‑69 (MQ=255)

                                                         gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:5940682/1‑69 (MQ=255)

                                                         gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGa            <  1:5880571/71‑1 (MQ=255)

                                                          tCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAg           >  1:4168050/1‑71 (MQ=255)

                                                          tCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCG‑GGGAATAATTACGCTTGTCGAg           >  1:3609793/1‑70 (MQ=255)

                                                           cGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGc          >  1:1313101/1‑71 (MQ=255)

                                                             ttGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:2561030/55‑1 (MQ=255)

                                                             ttGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:2793624/55‑1 (MQ=255)

                                                              tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGca                  >  1:464825/1‑59 (MQ=255)

                                                              tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:2654202/1‑70 (MQ=255)

                                                              tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:5219262/1‑70 (MQ=255)

                                                              tgtgTTTACCACCCGCACGCACGCAGAGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:5813680/1‑70 (MQ=255)

                                                               gtgtTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGATTAATTACGCTTGTCGAGccc        >  1:1595110/1‑69 (MQ=255)

                                                               gtgtTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:5621764/1‑69 (MQ=255)

                                                                tgtTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCaa      >  1:6351743/1‑70 (MQ=255)

                                                                 gtTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAg     >  1:3514400/1‑70 (MQ=255)

                                                                    tACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCa       >  1:4265336/1‑65 (MQ=255)

                                                                     accaccCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:5158313/47‑1 (MQ=255)

GTTGCCCAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAA  >  minE/1814889‑1815026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`