Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,814,961 A→G 5.1% G72G (GGT→GGC)  alaS ← alanyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,814,9610AG 5.1% 422.5 / 10.2 138G72G (GGT→GGC) alaSalanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (71/60);  new base G (4/3);  total (75/63)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 144 total aligned reads displayed.

CAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAA  >  minE/1814895‑1815026
                                                                  |                                                                 
cAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTGCAGGTCGTTGTGTTTACCACCcg                                                               >  1:4087823/1‑71 (MQ=255)
  gCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                             >  1:1049320/1‑71 (MQ=255)
  gCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                             >  1:694523/1‑71 (MQ=255)
       tCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcac                                                        >  1:4279085/1‑71 (MQ=255)
       tCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcac                                                        >  1:3626822/1‑71 (MQ=255)
         gaagaaGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgcacgc                                                      <  1:6704483/71‑1 (MQ=255)
           agaagGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTaccac                                                                  >  1:2273620/1‑57 (MQ=255)
                 tATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTgg                                              <  1:2610213/71‑1 (MQ=255)
                   tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTaccac                                                                  <  1:2908878/49‑1 (MQ=255)
                   tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTaccac                                                                  <  1:2011103/49‑1 (MQ=255)
                   tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:1708652/70‑1 (MQ=255)
                   tGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:2460648/70‑1 (MQ=255)
                    ggTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTaccac                                                                  <  1:6100423/48‑1 (MQ=255)
                     gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCAccc                                                                >  1:3751785/1‑49 (MQ=255)
                     gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgcacgc                                                          <  1:4536589/55‑1 (MQ=255)
                     gTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGaa                                           <  1:4904720/70‑1 (MQ=255)
                      tGACGCGCAGTGTAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGCGCTggacc                                           >  1:6776399/1‑66 (MQ=255)
                         cgcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGt                                      <  1:2611455/71‑1 (MQ=255)
                         cgcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCAGCACGCACGCAGCGTTGGGAAGTGGt                                      <  1:6912977/71‑1 (MQ=255)
                          gcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTgg                                       >  1:5310989/1‑69 (MQ=255)
                          gcgcgGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGAAGCGTTGGGAAGTgg                                       >  1:2047235/1‑69 (MQ=255)
                              agtgtAGCCGATGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGc                                                   <  1:6027624/52‑1 (MQ=255)
                              agtgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGc                                                   <  1:693214/52‑1 (MQ=255)
                               gtgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACACGCACGCACGCAGCGTTGGGAAGTGGTAGcgcg                                >  1:3290043/1‑71 (MQ=255)
                                tgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGCgc                                                 >  1:5933786/1‑52 (MQ=255)
                                tgtAGCCGACGTTTTCCAGGTCGTTGTGCTTGCCGCCGGCGCGCACGCAGCg                                                  >  1:4721570/1‑52 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:2434677/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:1440566/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:772218/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:3584305/53‑1 (MQ=255)
                                tgtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 <  1:5084803/53‑1 (MQ=255)
                                 gtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                             >  1:5368208/1‑40 (MQ=255)
                                 gtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCcgca                                                             >  1:3535069/1‑40 (MQ=255)
                                 gtAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCgg                               >  1:1630240/1‑70 (MQ=255)
                                  tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:3077360/55‑1 (MQ=255)
                                  tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:870724/55‑1 (MQ=255)
                                  tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTggg                                             <  1:6745771/55‑1 (MQ=255)
                                  tAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCggg                              >  1:3939106/1‑70 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:3306027/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:6525830/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:2810246/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:1404932/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:2769467/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGt                                                 >  1:6901468/1‑49 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           <  1:3458024/71‑1 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           <  1:5255385/71‑1 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            >  1:5000761/1‑70 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            >  1:5100965/1‑70 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            >  1:2317881/1‑70 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            <  1:5396176/70‑1 (MQ=255)
                                    aCCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            <  1:3151176/70‑1 (MQ=255)
                                    aCCGACGTTTTCCAGCTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaa                            >  1:5959100/1‑70 (MQ=255)
                                     cctaCGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGcgcg                                <  1:377531/62‑1 (MQ=255)
                                     ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                          <  1:1030993/71‑1 (MQ=255)
                                     ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaata                          <  1:6662543/71‑1 (MQ=255)
                                     ccGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           >  1:1957957/1‑70 (MQ=255)
                                       gACGTTTTCCATGTAGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:2549287/71‑1 (MQ=255)
                                        aCGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        >  1:5447127/1‑70 (MQ=255)
                                        aCGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        >  1:4946847/1‑70 (MQ=255)
                                          gctttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           <  1:4376835/63‑1 (MQ=255)
                                           ttttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaat                           <  1:4441685/64‑1 (MQ=255)
                                           ttttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACg                    <  1:2849518/71‑1 (MQ=255)
                                           ttttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACg                    <  1:6309461/71‑1 (MQ=255)
                                             ttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCt                  >  1:768313/1‑71 (MQ=255)
                                             ttCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCt                  >  1:6867450/1‑71 (MQ=255)
                                               ccAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCtt                 <  1:1205094/70‑1 (MQ=255)
                                               ccAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCtt                 <  1:2747321/70‑1 (MQ=255)
                                                cAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGt               <  1:4984460/71‑1 (MQ=255)
                                                  ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGTGAAGTGGTAGCGCGGGAATAATTACGCTTGTCg             <  1:6218943/71‑1 (MQ=255)
                                                  ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:4971532/1‑70 (MQ=255)
                                                  ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:5320192/1‑70 (MQ=255)
                                                  ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:2443835/1‑70 (MQ=255)
                                                  ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCg             <  1:948317/71‑1 (MQ=255)
                                                  ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCg             <  1:2089643/71‑1 (MQ=255)
                                                  ggTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCg             <  1:6474001/71‑1 (MQ=255)
                                                   gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:5940682/1‑69 (MQ=255)
                                                   gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:1663899/1‑69 (MQ=255)
                                                   gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:6207465/1‑69 (MQ=255)
                                                   gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTc              >  1:6329873/1‑69 (MQ=255)
                                                   gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGa            <  1:3188644/71‑1 (MQ=255)
                                                   gTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGa            <  1:5880571/71‑1 (MQ=255)
                                                    tCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAg           <  1:5113619/71‑1 (MQ=255)
                                                    tCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAg           >  1:4168050/1‑71 (MQ=255)
                                                    tCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCG‑GGGAATAATTACGCTTGTCGAg           >  1:3609793/1‑70 (MQ=255)
                                                     cGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGc          >  1:1313101/1‑71 (MQ=255)
                                                       ttGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:5711060/55‑1 (MQ=255)
                                                       ttGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:2793624/55‑1 (MQ=255)
                                                       ttGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:2561030/55‑1 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGca                  >  1:464825/1‑59 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:1059950/1‑70 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:2654202/1‑70 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:3382939/1‑70 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:5219262/1‑70 (MQ=255)
                                                        tgtgTTTACCACCCGCACGCACGCAGAGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:5813680/1‑70 (MQ=255)
                                                         gtgtTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGccc        >  1:5621764/1‑69 (MQ=255)
                                                          tgtTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCaa      >  1:6351743/1‑70 (MQ=255)
                                                            tttACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAgg    <  1:3916275/70‑1 (MQ=255)
                                                              tACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCa       >  1:4265336/1‑65 (MQ=255)
                                                              tACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGaa  >  1:3667977/1‑70 (MQ=255)
                                                               accaccCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGaataat                        <  1:5158313/47‑1 (MQ=255)
                                                                  |                                                                 
CAGCATTTCGAAGAAGGTATGGTGACGCGCGGTGTAACCGACGTTTTCCAGGTCGTTGTGTTTACCACCCGCACGCACGCAGCGTTGGGAAGTGGTAGCGCGGGAATAATTACGCTTGTCGAGCCCAAGGAA  >  minE/1814895‑1815026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑