| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,064,080 | C→G | 6.5% | T107T (ACC→ACG) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,064,080 | 0 | C | G | 6.5% | 304.3 / 8.6 | 92 | T107T (ACC→ACG) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base C (59/27); new base G (2/4); total (61/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
TTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTC > minE/2064010‑2064150 | tttCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGGAcc > 1:5506179/1‑71 (MQ=255)tttCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCAcc > 1:3976680/1‑71 (MQ=255)tttCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCAcc > 1:129816/1‑71 (MQ=255)tttCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCAcc > 1:1645377/1‑71 (MQ=255)tttCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCAcc > 1:3762845/1‑71 (MQ=255)tttCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCAcc > 1:3651550/1‑71 (MQ=255) ccAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGtt > 1:678672/1‑71 (MQ=255) ccAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGtt > 1:2834270/1‑71 (MQ=255) cAGCGTGGAATCTTAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTg < 1:6506342/71‑1 (MQ=255) gCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATATACATGCGTGAAATGGGCACCGTTGaa > 1:1744990/1‑71 (MQ=255) gTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACt > 1:4532968/1‑71 (MQ=255) gTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACt < 1:4393210/71‑1 (MQ=255) gTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACt < 1:5583495/71‑1 (MQ=255) gTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACt < 1:3010090/71‑1 (MQ=255) gTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGATGAACt < 1:886874/71‑1 (MQ=255) tGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTg > 1:823330/1‑71 (MQ=255) aaTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTg < 1:1877256/71‑1 (MQ=255) aTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACt > 1:6254662/1‑66 (MQ=255) aTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACt > 1:5988735/1‑66 (MQ=255) aTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACt > 1:5903568/1‑66 (MQ=255) aTCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACt > 1:3232491/1‑66 (MQ=255) tCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTg < 1:6277889/61‑1 (MQ=255) tCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTg < 1:3238560/61‑1 (MQ=255) tGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGAccc > 1:1193875/1‑71 (MQ=255) aaaTCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGaa > 1:6274242/1‑59 (MQ=255) aaaTCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGaa > 1:2118820/1‑59 (MQ=255) aaTCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgc > 1:5743850/1‑70 (MQ=255) aaTCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgc > 1:3110790/1‑70 (MQ=255) cGGCCGCACCACCGACCCGGTCCGCATGTACATGCGCGAGATGGGCGCGGTGTCGCTGCTGACGCGCGAAg > 1:4729303/1‑71 (MQ=255) gggCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAgg > 1:5016737/1‑71 (MQ=255) gcgcACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGc < 1:6257689/70‑1 (MQ=255) cgcACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGa < 1:6080671/71‑1 (MQ=255) aCGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGaa > 1:1699176/1‑48 (MQ=255) aCGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAAt > 1:4572189/1‑71 (MQ=255) cGACTGACCCGGTACTCATGTACATGCGTGAAATGGGCACCGTTGAACt < 1:5591224/49‑1 (MQ=255) gACTGACCCGGTACGCATGTACATGCGTGAAATGGGTACCGTTGAACTGTTGACCCGCGAAGGCGAAATTg < 1:3514912/71‑1 (MQ=255) gACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTg < 1:1976413/71‑1 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa > 1:1759616/1‑70 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa > 1:5067565/1‑70 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa > 1:1989583/1‑70 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa > 1:5769790/1‑70 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGAc < 1:6385041/71‑1 (MQ=255) aCCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATc > 1:519326/1‑71 (MQ=255) cccGGTCCGCATGTACATGCGCGAGATGGGCGCGGTGTCGCTGCTGACGCGCGAAGGCGagatcgagatcg < 1:3969888/71‑1 (MQ=255) cccGGTCCGCATGTACATGCGCGAGATGGGCGCGGTGTCGCTGCTGACGCGCGAAGGCGagatcgagatcg < 1:2558098/71‑1 (MQ=255) cccGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCg < 1:5218307/71‑1 (MQ=255) ccGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGc > 1:5151413/1‑71 (MQ=255) ggTCCGCATGTATATGCGCGAGATGGGCTCGGTCGAGCTGCTCACGCGCGAAGGCGAGATCGAGATCGcga < 1:2749929/71‑3 (MQ=255) ggTCCGCATGTATATGCGCGAGATGGGCTCGGTCGAGCTGCTCACGCGCGAAGGCGAGATCGAGATCGcga < 1:4710248/71‑3 (MQ=255) ggTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTa < 1:1136591/71‑1 (MQ=255) cGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCg < 1:2212401/71‑1 (MQ=255) gCATGTACATGCGCGAGATGGGCGCGGTGTCGCTGCTGACGCGCGAAGGCGAGATCGAGATCGCCAAGCg > 1:4794699/1‑70 (MQ=255) tGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAtt > 1:648202/1‑71 (MQ=255) gTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAtt > 1:4802033/1‑70 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaaa > 1:5745828/1‑49 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaaa > 1:4381273/1‑49 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaaa > 1:112708/1‑49 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaaa > 1:1163336/1‑49 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaaa > 1:6827173/1‑49 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGa < 1:4693496/71‑1 (MQ=255) aCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg > 1:3931574/1‑69 (MQ=255) aCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg > 1:550127/1‑69 (MQ=255) aCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg > 1:2275490/1‑69 (MQ=255) aCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg > 1:1748459/1‑69 (MQ=255) cATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgcg < 1:2722520/38‑1 (MQ=255) cATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTa > 1:3862035/1‑65 (MQ=255) gCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACg > 1:5501822/1‑71 (MQ=255) gCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACg > 1:3237163/1‑71 (MQ=255) gCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACg > 1:4387304/1‑71 (MQ=255) cGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAg > 1:5343440/1‑37 (MQ=255) cGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAg > 1:1877126/1‑37 (MQ=255) cGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAg > 1:6608286/1‑37 (MQ=255) cGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAg > 1:2489841/1‑37 (MQ=255) gTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa < 1:2718488/46‑1 (MQ=255) gTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACggg < 1:4926132/71‑1 (MQ=255) gTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACgg > 1:5131990/1‑70 (MQ=255) gTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACgg > 1:2610697/1‑70 (MQ=255) gAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACa > 1:4439339/1‑46 (MQ=255) gAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACa > 1:5451769/1‑46 (MQ=255) aaaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGAt > 1:2570542/1‑70 (MQ=255) aaaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGAt > 1:4077446/1‑70 (MQ=255) aaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATc > 1:3640458/1‑70 (MQ=255) aaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATc > 1:5389874/1‑70 (MQ=255) aaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATc > 1:6543466/1‑70 (MQ=255) aaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATc > 1:503257/1‑70 (MQ=255) tGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCa < 1:3682112/69‑1 (MQ=255) tGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCa < 1:6633275/69‑1 (MQ=255) gCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAg < 1:586824/71‑1 (MQ=255) gCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAg < 1:4979151/71‑1 (MQ=255) ccGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg < 1:980058/51‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:941492/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:94000/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:2907331/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:680362/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:6797563/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:2463551/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:2488474/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTc < 1:2521589/71‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCATATTGAAGACGGGATCAACCAGGTTc < 1:4017084/71‑1 (MQ=255) cGTTGAACTGTTGACACGCGAAGGCGAAATTGACATCTCTAAGCGTATTGAAGACGGGATCAACCAGGTTc < 1:6208232/71‑1 (MQ=255) | TTTCCAGCGTGGAATCTGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTC > minE/2064010‑2064150 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |