| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,064,095 | C→G | 5.3% | T112T (ACC→ACG) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,064,095 | 0 | C | G | 5.3% | 452.3 / 10.1 | 132 | T112T (ACC→ACG) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base C (88/36); new base G (3/4); total (91/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01 | |||||||||||
Only 100 of 137 total aligned reads displayed.
TGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTG > minE/2064026‑2064165 | tGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGAccc > 1:1193875/1‑71 (MQ=255) aaaTCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgc > 1:656230/1‑71 (MQ=255) aaTCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgc > 1:3110790/1‑70 (MQ=255) cGGCCGCACCACCGACCCGGTCCGCATGTACATGCGCGAGATGGGCGCGGTGTCGCTGCTGACGCGCGAAg > 1:4729303/1‑71 (MQ=255) gggCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAgg > 1:5016737/1‑71 (MQ=255) gcgcACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGc < 1:6257689/70‑1 (MQ=255) cgcACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGa < 1:6080671/71‑1 (MQ=255) aCGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAAt > 1:4572189/1‑71 (MQ=255) gACTGACCCGGTACGCATGTACATGCGTGAAATGGGTACCGTTGAACTGTTGACCCGCGAAGGCGAAATTg < 1:3514912/71‑1 (MQ=255) gACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTg < 1:1976413/71‑1 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa > 1:2579748/1‑70 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa > 1:5769790/1‑70 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa > 1:1989583/1‑70 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa > 1:1950799/1‑70 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa > 1:1759616/1‑70 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa > 1:5067565/1‑70 (MQ=255) cTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGAc < 1:6385041/71‑1 (MQ=255) cccGGTCCGCATGTACATGCGCGAGATGGGCGCGGTGTCGCTGCTGACGCGCGAAGGCGagatcgagatcg < 1:3969888/71‑1 (MQ=255) cccGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCg < 1:5218307/71‑1 (MQ=255) ccGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGc > 1:5151413/1‑71 (MQ=255) ggTCCGCATGTATATGCGCGAGATGGGCTCGGTCGAGCTGCTCACGCGCGAAGGCGAGATCGAGATCGcga < 1:4710248/71‑3 (MQ=255) gTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAtt > 1:4802033/1‑70 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaaa > 1:112708/1‑49 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaaa > 1:1163336/1‑49 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaaa > 1:6827173/1‑49 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGaaa > 1:5745828/1‑49 (MQ=255) tACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg < 1:5396024/70‑1 (MQ=255) aCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg > 1:2275490/1‑69 (MQ=255) aCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg > 1:550127/1‑69 (MQ=255) aCATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTg > 1:1748459/1‑69 (MQ=255) cATGCGTGAAATGGGCACCGTTGAACTGTTGACCcgcg < 1:2722520/38‑1 (MQ=255) cATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTa > 1:3862035/1‑65 (MQ=255) gCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACg > 1:4387304/1‑71 (MQ=255) gCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACg > 1:3237163/1‑71 (MQ=255) cGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAg > 1:5343440/1‑37 (MQ=255) cGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAg > 1:1877126/1‑37 (MQ=255) cGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAg > 1:2489841/1‑37 (MQ=255) cGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAg > 1:594988/1‑37 (MQ=255) gTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGa < 1:2718488/46‑1 (MQ=255) gTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACggg < 1:4918071/71‑1 (MQ=255) gTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACggg < 1:4926132/71‑1 (MQ=255) gTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACgg > 1:5131990/1‑70 (MQ=255) gTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACgg > 1:2610697/1‑70 (MQ=255) gAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACa > 1:5451769/1‑46 (MQ=255) gAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACa > 1:4439339/1‑46 (MQ=255) aaaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGAt > 1:4077446/1‑70 (MQ=255) aaaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGAt > 1:2570542/1‑70 (MQ=255) aaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATc > 1:503257/1‑70 (MQ=255) aaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATc > 1:3640458/1‑70 (MQ=255) aaTGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATc > 1:6543466/1‑70 (MQ=255) tGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCa < 1:6633275/69‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:680362/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:94000/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:941492/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:2488474/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAg < 1:2907331/43‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTc < 1:2521589/71‑1 (MQ=255) cGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCATATTGAAGACGGGATCAACCAGGTTc < 1:4017084/71‑1 (MQ=255) gTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCg > 1:1630734/1‑37 (MQ=255) gTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGaa > 1:4003481/1‑51 (MQ=255) gTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGaa > 1:217083/1‑51 (MQ=255) gTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGaa > 1:3639039/1‑51 (MQ=255) gTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGaa > 1:1570786/1‑51 (MQ=255) gTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTc > 1:4283105/1‑70 (MQ=255) gTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTc > 1:3469940/1‑70 (MQ=255) ttGAACTGTTGACGCGCGAAGGCGAAATTGACATCGCTAAGCGTa > 1:3018553/1‑45 (MQ=255) ttGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTa > 1:5389163/1‑45 (MQ=255) ttGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTa > 1:6113002/1‑45 (MQ=255) ttGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTa > 1:2264239/1‑45 (MQ=255) ttGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTa > 1:2869096/1‑45 (MQ=255) ttGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTa > 1:6889997/1‑45 (MQ=255) gAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCa > 1:6033410/1‑62 (MQ=255) gAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATg > 1:341719/1‑71 (MQ=255) gAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATg > 1:1185318/1‑71 (MQ=255) aCTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCg > 1:6697374/1‑39 (MQ=255) aCTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCg > 1:3274140/1‑39 (MQ=255) aCTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCg > 1:1279028/1‑39 (MQ=255) aCTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCg > 1:6852131/1‑39 (MQ=255) aCTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCg > 1:6921258/1‑39 (MQ=255) cTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCaa > 1:4637324/1‑56 (MQ=255) cTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCaa > 1:1919442/1‑56 (MQ=255) cTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCaa > 1:5951432/1‑56 (MQ=255) cTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCaa > 1:5829399/1‑56 (MQ=255) cTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCaa > 1:3000200/1‑56 (MQ=255) cTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCaa > 1:3654748/1‑56 (MQ=255) cTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCa > 1:2725767/1‑55 (MQ=255) cTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTc < 1:4384115/71‑1 (MQ=255) tGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAt < 1:6214249/40‑1 (MQ=255) tGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTAt < 1:466031/40‑1 (MQ=255) tGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCaa > 1:6375583/1‑65 (MQ=255) tGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCaa > 1:6383277/1‑65 (MQ=255) tGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCaa > 1:3684364/1‑65 (MQ=255) tGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTcc > 1:6135116/1‑71 (MQ=255) gTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCg > 1:1074117/1‑71 (MQ=255) ttGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGt < 1:1000001/71‑1 (MQ=255) gACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGa > 1:770767/1‑42 (MQ=255) gACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGa > 1:5646005/1‑42 (MQ=255) gACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGtt < 1:6564360/70‑1 (MQ=255) aCCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATc < 1:3916321/48‑1 (MQ=255) ccGCGAAGGCGAAATT‑ACATCGCTAAGCGTATTGaa < 1:5638773/36‑1 (MQ=38) | TGAAATCGGGCGCACGACTGACCCGGTACGCATGTACATGCGTGAAATGGGCACCGTTGAACTGTTGACCCGCGAAGGCGAAATTGACATCGCTAAGCGTATTGAAGACGGGATCAACCAGGTTCAATGCTCCGTTGCTG > minE/2064026‑2064165 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |