| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,065,001 | A→G | 5.5% | K414K (AAA→AAG) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,065,001 | 0 | A | G | 5.5% | 490.8 / 15.2 | 163 | K414K (AAA→AAG) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base A (89/65); new base G (4/5); total (93/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
Only 100 of 170 total aligned reads displayed.
AAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAG > minE/2064933‑2065070 | aaGAAATAGACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAg > 1:4731039/1‑70 (MQ=255)aaGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGc > 1:3295182/1‑71 (MQ=255)aaGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGc > 1:231214/1‑71 (MQ=255)aaGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGc > 1:6041457/1‑71 (MQ=255) aGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCg > 1:3669903/1‑71 (MQ=255) aaTACACCAACCGTGGCTTGCAGTTCCTTTACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt < 1:4216176/71‑1 (MQ=255) aaTACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt < 1:5053871/71‑1 (MQ=255) aTACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:1079908/1‑70 (MQ=255) aTACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:3788427/1‑70 (MQ=255) aTACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:5684646/1‑70 (MQ=255) acCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaa < 1:1101925/71‑1 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAATGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:1029277/69‑1 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGaaa > 1:2643896/1‑59 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCTGTTGATa < 1:1355618/69‑1 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa < 1:1062831/71‑1 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:2005012/69‑1 (MQ=255) cAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGa > 1:6407636/1‑66 (MQ=255) cAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAAt > 1:659923/1‑71 (MQ=255) ccGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:627763/71‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:3884138/62‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:650810/62‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATa < 1:5697636/70‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATa < 1:928443/70‑1 (MQ=255) gCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAccgcc > 1:1719058/1‑71 (MQ=255) gCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAccgcc > 1:486590/1‑71 (MQ=255) caattCCTCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATAccgtcg > 1:1226268/4‑71 (MQ=16) caattCCTCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATAccgtcg > 1:593885/4‑71 (MQ=16) caattCCTCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATAccgtcg > 1:181924/4‑71 (MQ=16) cAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAccgccg < 1:1642067/71‑1 (MQ=255) gTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTg > 1:2339213/1‑71 (MQ=255) ttCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTgg > 1:2477734/1‑71 (MQ=255) tCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGt > 1:1931985/1‑46 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTg > 1:4948457/1‑47 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTg > 1:4288257/1‑47 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTg > 1:3479955/1‑47 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTg > 1:3773531/1‑47 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:5387391/1‑52 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:1324349/1‑52 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:353584/1‑52 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGt > 1:2903113/1‑70 (MQ=255) cTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGtt < 1:4126821/70‑1 (MQ=255) ttGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTa > 1:5458637/1‑70 (MQ=255) tGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTAc > 1:2791913/1‑70 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGa > 1:5637550/1‑44 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGa > 1:691130/1‑44 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:6305556/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:5023907/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTgg > 1:4675642/1‑65 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTgg > 1:296902/1‑65 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTg > 1:849608/1‑64 (MQ=255) aCCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg < 1:6198136/71‑1 (MQ=255) ccTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATaaat < 1:5919113/71‑3 (MQ=12) cTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGt < 1:3069935/70‑1 (MQ=255) cTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGt < 1:4970678/70‑1 (MQ=255) cTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATaaat < 1:6131862/70‑3 (MQ=12) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAACGCCGTGGTTACAAGTtct > 1:3737742/1‑71 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:2879053/1‑36 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:2594903/1‑36 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:2981297/1‑36 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTct < 1:6185135/46‑2 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtc < 1:5334154/69‑1 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGCTGATAAATTCg < 1:4995951/46‑1 (MQ=255) ccAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGTTATAAATtctcg > 1:2251713/2‑69 (MQ=255) aGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCAc < 1:5898393/70‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:1301026/71‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:3936255/71‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:5725593/71‑1 (MQ=255) gAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTa < 1:5372516/71‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:6151418/37‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:470093/37‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:452616/37‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:555968/37‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:799211/37‑1 (MQ=255) cAACATCGGTCTAATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCaa < 1:3961382/71‑1 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:4809046/59‑1 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:1507474/59‑1 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAcc < 1:1828410/71‑1 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAcc > 1:3930994/1‑71 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtg > 1:1432374/1‑71 (MQ=255) tCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:4849767/69‑1 (MQ=255) ggTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCaa > 1:2589102/1‑64 (MQ=255) ggTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtggtg > 1:6804802/1‑71 (MQ=255) tCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtggtg > 1:663752/1‑69 (MQ=255) tCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtggtg > 1:6143565/1‑69 (MQ=255) cTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGAt > 1:3210071/1‑71 (MQ=255) cTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGAt > 1:276128/1‑71 (MQ=255) tgatgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCa < 1:199756/59‑1 (MQ=255) gatgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:4035737/53‑1 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtctc > 1:3319673/1‑47 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCa > 1:5670959/1‑49 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAc > 1:4415569/1‑59 (MQ=255) tgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGc > 1:4294136/1‑55 (MQ=255) tgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTTGATCCGt < 1:4784723/71‑1 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCCCCTACGCAACCTGGTGGATCCGt > 1:5502235/1‑70 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGt > 1:3396338/1‑70 (MQ=255) aaaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTc < 1:1568276/70‑1 (MQ=255) aaaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTc < 1:6107662/70‑1 (MQ=255) aaaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTc < 1:2745295/70‑1 (MQ=255) aaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAg > 1:5084517/1‑71 (MQ=255) aaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAg > 1:4452565/1‑71 (MQ=255) | AAGAAATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAG > minE/2064933‑2065070 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |