| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,065,007 | T→G | 5.7% | V416V (GTT→GTG) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,065,007 | 0 | T | G | 5.7% | 448.2 / 15.1 | 155 | V416V (GTT→GTG) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base T (79/67); new base G (4/5); total (83/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 178 total aligned reads displayed.
AATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCA > minE/2064937‑2065077 | aaTACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt < 1:5053871/71‑1 (MQ=255) aTACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:1079908/1‑70 (MQ=255) aTACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:6876392/1‑70 (MQ=255) aTACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:3788427/1‑70 (MQ=255) aTACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:4820853/1‑70 (MQ=255) acacCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGa > 1:5673221/1‑70 (MQ=255) cacCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:1236955/71‑1 (MQ=255) acCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaa < 1:1101925/71‑1 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAATGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:1029277/69‑1 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa < 1:1062831/71‑1 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:2005012/69‑1 (MQ=255) ccGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:1357052/71‑1 (MQ=255) ccGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:627763/71‑1 (MQ=255) tGGCTTGGAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:365657/62‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:6052850/62‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:3884138/62‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:650810/62‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATa < 1:4265932/70‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATa < 1:5697636/70‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATa < 1:928443/70‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATa < 1:3531724/70‑1 (MQ=255) gCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAccgcc > 1:1719058/1‑71 (MQ=255) gCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAccgcc > 1:486590/1‑71 (MQ=255) gCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAccgc > 1:3328902/1‑70 (MQ=255) caattCCTCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATAccgtcg > 1:181924/4‑71 (MQ=16) caattCCTCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATAccgtcg > 1:593885/4‑71 (MQ=16) cAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAccgccg < 1:1642067/71‑1 (MQ=255) aGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:5513543/54‑1 (MQ=255) ttCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTgg > 1:2477734/1‑71 (MQ=255) ccTTGACCTGATTCAGGAAGGCAGCATCGGTCTGATGAAAGCGGTTg > 1:5899465/1‑47 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTg > 1:2745915/1‑47 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTg > 1:4595386/1‑47 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTg > 1:4948457/1‑47 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:5387391/1‑52 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:353584/1‑52 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:1324349/1‑52 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGt > 1:2903113/1‑70 (MQ=255) cTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGaa < 1:5050473/57‑1 (MQ=255) cTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGtt < 1:3299949/70‑1 (MQ=255) ttGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTa > 1:5458637/1‑70 (MQ=255) tGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTAc > 1:2791913/1‑70 (MQ=255) gACCTGATTCAGGATGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:6434216/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGa > 1:691130/1‑44 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:5023907/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:476270/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:3934440/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:3189736/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:6305556/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTgg > 1:4675642/1‑65 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTg > 1:849608/1‑64 (MQ=255) cTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGt < 1:3069935/70‑1 (MQ=255) cTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATaaat < 1:6131862/70‑3 (MQ=12) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtct > 1:4451688/1‑71 (MQ=255) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtc > 1:5464138/1‑70 (MQ=255) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAACGCCGTGGTTACAAGTtct > 1:3737742/1‑71 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:2594903/1‑36 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:2879053/1‑36 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:2981297/1‑36 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGtt > 1:5646510/1‑36 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtc < 1:1970486/69‑1 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGCTGATAAATTCg < 1:4995951/46‑1 (MQ=255) ccAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGTTATAAATtctcg > 1:2251713/2‑69 (MQ=255) aGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCAc < 1:5898393/70‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:6186843/71‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:1301026/71‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:5725593/71‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:4786549/71‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:4123415/71‑1 (MQ=255) aaGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTAc < 1:6587845/71‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:2959563/37‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:555968/37‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:470093/37‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:799211/37‑1 (MQ=255) gCAACATCGTTCTGATGATAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCa < 1:377667/71‑1 (MQ=255) gCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCa < 1:5347566/71‑1 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:1507474/59‑1 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAcc < 1:1828410/71‑1 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtg > 1:1432374/1‑71 (MQ=255) ggTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCaa > 1:2589102/1‑64 (MQ=255) ggTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtggtg > 1:2659628/1‑71 (MQ=255) ggTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtggtg > 1:6804802/1‑71 (MQ=255) tCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtct > 1:416159/1‑50 (MQ=255) tCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtggtg > 1:3948830/1‑69 (MQ=255) tCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtggtg > 1:663752/1‑69 (MQ=255) tCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtggtg > 1:4958472/1‑69 (MQ=255) cTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtgg > 1:15568/1‑66 (MQ=255) gatgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:4035737/53‑1 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACaac > 1:5141410/1‑41 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtctc > 1:6143990/1‑47 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCa > 1:6260242/1‑49 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:6137594/61‑1 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:2327259/61‑1 (MQ=255) tgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGc > 1:4294136/1‑55 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGt > 1:3396338/1‑70 (MQ=255) aaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAg > 1:4452565/1‑71 (MQ=255) gTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:2329383/52‑1 (MQ=255) gTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGa > 1:3019055/1‑70 (MQ=255) gTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGAt > 1:6370581/1‑71 (MQ=255) ttGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATc < 1:5651283/71‑1 (MQ=255) tGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCa > 1:6151798/1‑71 (MQ=255) | AATACACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCA > minE/2064937‑2065077 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |