| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,065,010 | T→C | 5.4% | D417D (GAT→GAC) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,065,010 | 0 | T | C | 5.4% | 456.0 / 12.5 | 149 | D417D (GAT→GAC) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base T (72/69); new base C (3/5); total (75/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
Only 100 of 157 total aligned reads displayed.
CACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACC > minE/2064941‑2065079 | cacCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:1236955/71‑1 (MQ=255) acCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaa < 1:1101925/71‑1 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAATGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:1029277/69‑1 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa < 1:1062831/71‑1 (MQ=255) ccAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:2005012/69‑1 (MQ=255) ccGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:627763/71‑1 (MQ=255) ccGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:1357052/71‑1 (MQ=255) tGGCTTGGAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:365657/62‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:650810/62‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:6052850/62‑1 (MQ=255) tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:3884138/62‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:1098499/1‑62 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATa < 1:4265932/70‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATa < 1:5697636/70‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATa < 1:3531724/70‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATa < 1:1737267/70‑1 (MQ=255) gCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAccgcc > 1:486590/1‑71 (MQ=255) gCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAccgc > 1:3328902/1‑70 (MQ=255) caattCCTCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATAccgtcg > 1:181924/4‑71 (MQ=16) caattCCTCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATAccgtcg > 1:1226268/4‑71 (MQ=16) caattCCTCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATAccgtcg > 1:593885/4‑71 (MQ=16) cAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAccgccg < 1:1642067/71‑1 (MQ=255) aGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATa < 1:5513543/54‑1 (MQ=255) ttCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTgg > 1:2477734/1‑71 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:1324349/1‑52 (MQ=255) cTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGaa < 1:5786319/57‑1 (MQ=255) cTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGaa < 1:2885371/57‑1 (MQ=255) cTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGtt < 1:3299949/70‑1 (MQ=255) cTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGtt < 1:4126821/70‑1 (MQ=255) ttGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTa > 1:5458637/1‑70 (MQ=255) tGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTAc > 1:298290/1‑70 (MQ=255) gACCTGATTCAGGATGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:6434216/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:476270/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:1304002/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:6305556/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGAt > 1:3934440/1‑45 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTgg > 1:4675642/1‑65 (MQ=255) gACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTg > 1:849608/1‑64 (MQ=255) ccTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATaaat < 1:2275039/71‑3 (MQ=12) cTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGt < 1:3069935/70‑1 (MQ=255) cTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGt < 1:4970678/70‑1 (MQ=255) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtc > 1:5464138/1‑70 (MQ=255) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAACGCCGTGGTTACAAGTtct > 1:3737742/1‑71 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:1921343/46‑1 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtc < 1:1970486/69‑1 (MQ=255) aTTCAGGAAGGCAACATCGGTCTGATGAAAGCGGCTGATAAATTCg < 1:4995951/46‑1 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCa > 1:986200/1‑71 (MQ=255) ccAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGTTATAAATtctcg > 1:2251713/2‑69 (MQ=255) aGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCAc < 1:5898393/70‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:3936255/71‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:6186843/71‑1 (MQ=255) ggAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:4786549/71‑1 (MQ=255) gAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTa < 1:2127993/71‑1 (MQ=255) gAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTCGACCTa < 1:5372516/71‑1 (MQ=255) aaGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTAc < 1:6587845/71‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:452616/37‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:6151418/37‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:555968/37‑1 (MQ=255) ggCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:2959563/37‑1 (MQ=255) gCAACATCGTTCTGATGATAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCa < 1:377667/71‑1 (MQ=255) gCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCa < 1:5347566/71‑1 (MQ=255) cAACATCGGTCTAATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCaa < 1:3961382/71‑1 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:4809046/59‑1 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:1507474/59‑1 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtg > 1:1432374/1‑71 (MQ=255) ggTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCaa > 1:2589102/1‑64 (MQ=255) tCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtggtg > 1:663752/1‑69 (MQ=255) tCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtggtg > 1:6143565/1‑69 (MQ=255) cTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCAcc < 1:2851911/54‑1 (MQ=255) cTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGAt > 1:3210071/1‑71 (MQ=255) cTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGAt > 1:276128/1‑71 (MQ=255) gatgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:4035737/53‑1 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACaac > 1:5141410/1‑41 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCa > 1:6260242/1‑49 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAc > 1:4415569/1‑59 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:6137594/61‑1 (MQ=255) tgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGt > 1:4890408/1‑71 (MQ=255) tgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGt < 1:4682136/71‑1 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGt > 1:3396338/1‑70 (MQ=255) aaaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTc < 1:1568276/70‑1 (MQ=255) aaaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTc < 1:2745295/70‑1 (MQ=255) aaaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTc < 1:6107662/70‑1 (MQ=255) aaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAg > 1:4452565/1‑71 (MQ=255) aaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAg > 1:5084517/1‑71 (MQ=255) gCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGc > 1:6820629/1‑71 (MQ=255) cGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCg < 1:5050419/71‑1 (MQ=255) ggTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGa > 1:3643125/1‑71 (MQ=255) gTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:2329383/52‑1 (MQ=255) gTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGa > 1:6370412/1‑70 (MQ=255) ttGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGAt > 1:767246/1‑70 (MQ=255) ttGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATc < 1:5651283/71‑1 (MQ=255) tGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACg > 1:302082/1‑44 (MQ=255) tGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCa > 1:6151798/1‑71 (MQ=255) gATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAc > 1:3016382/1‑47 (MQ=255) gATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAc > 1:5692588/1‑47 (MQ=255) gATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAc > 1:6687419/1‑47 (MQ=255) gATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAc > 1:4131421/1‑47 (MQ=255) gATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAc > 1:1854702/1‑47 (MQ=255) gATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCAc > 1:90264/1‑71 (MQ=255) gATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCAc > 1:4425978/1‑71 (MQ=255) | CACCAACCGTGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCGATCACC > minE/2064941‑2065079 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |