Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,139,806 T→G 11.1% E434D (GAA→GAC infB ← fused protein chain initiation factor 2, IF2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,139,8060TG11.1% 255.8 / 31.7 108E434D (GAA→GACinfBfused protein chain initiation factor 2, IF2
Reads supporting (aligned to +/- strand):  ref base T (50/46);  new base G (6/6);  total (56/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGA  >  minE/2139737‑2139875
                                                                     |                                                                     
acGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGttt                                                                      >  1:106908/1‑71 (MQ=255)
acGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGttt                                                                      >  1:6226096/1‑71 (MQ=255)
acGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTAAGttt                                                                      >  1:723294/1‑71 (MQ=255)
 cGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGttt                                                                      >  1:3377095/1‑70 (MQ=255)
 cGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGtt                                                                       <  1:6590845/69‑1 (MQ=255)
 cGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGtt                                                                       <  1:3974775/69‑1 (MQ=255)
      aCGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGt                                                                <  1:3710901/71‑1 (MQ=255)
       cGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTg                                                               <  1:1163246/71‑1 (MQ=255)
        gCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACg                                                                 <  1:1467669/68‑1 (MQ=255)
        gCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACg                                                                 <  1:1133788/68‑1 (MQ=255)
        gCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACg                                                                 <  1:6159332/68‑1 (MQ=255)
        gCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTgg                                                              <  1:4688673/71‑1 (MQ=255)
        gCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTgg                                                              <  1:699967/71‑1 (MQ=255)
        gCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTgg                                                              <  1:3331798/71‑1 (MQ=255)
            tGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTAt                                                           >  1:6136878/1‑70 (MQ=255)
            tGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTAt                                                           >  1:5201502/1‑70 (MQ=255)
            tGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATg                                                          >  1:1099760/1‑71 (MQ=255)
             gAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATg                                                          >  1:5957997/1‑70 (MQ=255)
             gAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGATTCa                                                                    >  1:1889213/1‑60 (MQ=255)
                 tAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCa                                                     <  1:5231141/71‑1 (MQ=255)
                   aaCGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAAc                                                                  <  1:2059592/56‑1 (MQ=255)
                   aaCGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCaa                                                    >  1:3081350/1‑70 (MQ=255)
                   aaCGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAAt                                                   >  1:2090403/1‑71 (MQ=255)
                   aaCGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAAt                                                   >  1:1579734/1‑71 (MQ=255)
                          cgTGCCCCGGGGTTTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTgg                                            <  1:537073/71‑1 (MQ=255)
                           gTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTggg                                           >  1:4854365/1‑71 (MQ=255)
                           gTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTgg                                            <  1:559571/70‑1 (MQ=255)
                              ccccGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCa                                                                    <  1:3724527/43‑1 (MQ=255)
                              ccccGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCa                                                                    <  1:1628170/43‑1 (MQ=255)
                               cccGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGt                                          <  1:5705774/68‑1 (MQ=255)
                               cccGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGt                                          <  1:3543110/68‑1 (MQ=255)
                                ccGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATg                                      >  1:4339812/1‑71 (MQ=255)
                                ccGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATg                                      >  1:785569/1‑71 (MQ=255)
                                 cGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:148114/1‑68 (MQ=255)
                                 cGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:633725/1‑68 (MQ=255)
                                 cGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:5291729/1‑68 (MQ=255)
                                        tCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTgg                                            >  1:4257694/1‑57 (MQ=255)
                                        tCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTgg                                            >  1:224402/1‑57 (MQ=255)
                                        tCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGt                                          <  1:3011127/59‑1 (MQ=255)
                                        tCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGt                                          <  1:469576/59‑1 (MQ=255)
                                        tCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGt                                          <  1:1158748/59‑1 (MQ=255)
                                        tCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGt                                          <  1:2093131/59‑1 (MQ=255)
                                        tCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGt                                          <  1:565233/59‑1 (MQ=255)
                                        tCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGccgcccgc                              >  1:5438716/1‑71 (MQ=255)
                                        tCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGccgcccgc                              >  1:762139/1‑71 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATg                                                          >  1:2860748/1‑41 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGc                                                         >  1:54236/1‑42 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCa                                                        >  1:149130/1‑43 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCaa                                                    >  1:1726062/1‑47 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCaa                                                    >  1:2356011/1‑47 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:6281625/1‑59 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:5391704/1‑59 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:1712895/1‑59 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCtt                            <  1:5751079/71‑1 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCtt                            <  1:5158040/71‑1 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCt                             <  1:5462838/70‑1 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCt                             <  1:6132464/70‑1 (MQ=255)
                                          cAGGAAGGTGATCATGCCGTTTTCACATTCAACGTCGTATGCACCaa                                                    >  1:2101757/1‑47 (MQ=255)
                                          aaGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCaa                                                    >  1:3020672/2‑47 (MQ=255)
                                           aGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCa                                                     <  1:3432033/45‑1 (MQ=255)
                                           aGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCa                                                     <  1:3282545/45‑1 (MQ=255)
                                           aGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCa                                                     <  1:4685742/45‑1 (MQ=255)
                                           aGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCa                                                     <  1:1232566/45‑1 (MQ=255)
                                           aGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCa                                                     <  1:2550598/45‑1 (MQ=255)
                                           aGGAAGGTGATCATGCCGCTCGGGGTGTCCACGTGGTATGCACCGATGTGCTGGGTAAt                                       <  1:5544043/59‑1 (MQ=255)
                                           aGGAAGGTGATCATGCCGCTCGGGGTGTCCACGTGGTATGCACCGATGTGCTGGGTAAt                                       <  1:5246850/59‑1 (MQ=255)
                                           aGGAAGGTGATCATGCCGCTCGGGGTGTCCACGTGGTATGCACCGATGTGCTGGGTAAt                                       <  1:6026663/59‑1 (MQ=255)
                                           aGGAAGGTGATCATGCCGCTCGGGGTGTCCACGTGGTATGCACCGATGTGCTGGGTAAt                                       <  1:1050516/59‑1 (MQ=255)
                                           aGGAAGGTGATCATGCCGCTCGGGGTGTCCACGTGGTATGCACCGATGTGCTGGGTAAt                                       <  1:6599479/59‑1 (MQ=255)
                                           aGGAAGGTGATCATGCCGCTCGGGGTGTCCACGTGGTATGCACCGATGTGCTGGGTAAt                                       <  1:246001/59‑1 (MQ=255)
                                             gAAGGTGATCATGCCGCTCGGGGTGTCCACGTGGTATGCACCGATGTGCTGGGTAATgc                                     >  1:5343265/1‑59 (MQ=255)
                                             gAAGGTGATCATGCCGCTCGGGGTGTCCACGTGGTATGCACCGATGTGCTGGGTAATgc                                     >  1:3979872/1‑59 (MQ=255)
                                             gAAGGTGATCATGCCGCTCGGGGTGTCCACGTGGTATGCACCGATGTGCTGGGTAATgc                                     >  1:3628665/1‑59 (MQ=255)
                                             gAAGGTGATCATGCCGCTCGGGGTGTCCACGTGGTATGCACCGATGTGCTGGGTAATgc                                     >  1:1749081/1‑59 (MQ=255)
                                             gAAGGTGATCATGCCGCTCGGGGTGTCAACGTGGTATGCACCGATGTGCTGGGTAATgc                                     >  1:4964495/1‑59 (MQ=255)
                                              aaGGTGATCATGCCGTTTTCAGTTTCAACGTGGTa                                                            >  1:2840867/1‑35 (MQ=255)
                                                ggTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCag                      <  1:827570/71‑1 (MQ=255)
                                                  tGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCagag                    <  1:4831167/71‑1 (MQ=255)
                                                  tGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCagag                    <  1:2598321/71‑1 (MQ=255)
                                                  tGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCagag                    <  1:6790402/71‑1 (MQ=255)
                                                    aTCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:6707217/1‑49 (MQ=255)
                                                    aTCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:382870/1‑49 (MQ=255)
                                                    aTCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:1051197/1‑49 (MQ=255)
                                                    aTCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:3937096/1‑49 (MQ=255)
                                                    aTCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:3570423/1‑49 (MQ=255)
                                                    aTCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:1302756/1‑49 (MQ=255)
                                                    aTCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTaa                                        >  1:3153766/1‑49 (MQ=255)
                                                    aTCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCaga                     <  1:2887602/68‑1 (MQ=255)
                                                          ccGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACtt             >  1:6198283/1‑70 (MQ=255)
                                                            gTTTTCAGTTTAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGt         <  1:2011960/71‑1 (MQ=255)
                                                             ttttCAGTTTCAACGTTGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCa                       <  1:1999596/57‑1 (MQ=255)
                                                             ttttCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCa                       <  1:489101/57‑1 (MQ=255)
                                                             ttttCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCa                       <  1:141455/57‑1 (MQ=255)
                                                               ttCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATgc                                     <  1:817385/41‑1 (MQ=255)
                                                                tCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTg       <  1:2963298/70‑1 (MQ=255)
                                                                tCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTg       <  1:167509/70‑1 (MQ=255)
                                                                tCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTg       <  1:1472039/70‑1 (MQ=255)
                                                                tCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTg       >  1:3429163/1‑70 (MQ=255)
                                                                tCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTg       >  1:4626479/1‑70 (MQ=255)
                                                                    tttCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGa  <  1:6728477/71‑1 (MQ=255)
                                                                     |                                                                     
ACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGTGGTATGCACCAATGTGCTGGGTAATGCCGCCCGCTTCGCCAGAGGCCACTTTCGTTGAACGA  >  minE/2139737‑2139875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: