| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,266,180 | A→G | 9.6% | Y88Y (TAT→TAC) | tufB ← | protein chain elongation factor EF‑Tu |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,266,180 | 0 | A | G | 9.6% | 237.2 / 14.7 | 83 | Y88Y (TAT→TAC) | tufB | protein chain elongation factor EF‑Tu |
| Reads supporting (aligned to +/- strand): ref base A (34/41); new base G (5/3); total (39/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.65e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
GCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACG > minE/2266111‑2266221 | gCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACAt < 1:957302/71‑1 (MQ=34) tCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc > 1:4802934/1‑71 (MQ=34) tCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc < 1:4269337/71‑1 (MQ=34) tCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc < 1:3212939/71‑1 (MQ=34) tCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTc < 1:1591960/71‑1 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:3002446/71‑1 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg > 1:6749481/1‑71 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg > 1:1463028/1‑71 (MQ=34) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg > 1:2431770/1‑70 (MQ=35) aGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:470721/70‑1 (MQ=35) gTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGc < 1:849833/71‑1 (MQ=34) tCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTGGGCg < 1:4715803/71‑1 (MQ=21) tCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCg < 1:2916673/71‑1 (MQ=34) cagcaACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGc < 1:1619526/71‑1 (MQ=34) caACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGcccc < 1:1301986/71‑1 (MQ=34) caACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGcccc < 1:525607/71‑1 (MQ=34) caACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGcccc < 1:2159842/71‑1 (MQ=34) caACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATCTTTTTAACATAGTCGGCg > 1:362186/1‑65 (MQ=255) aaCTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg > 1:2835901/1‑71 (MQ=34) aaCTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg > 1:3835567/1‑71 (MQ=34) aaCTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg < 1:4277662/71‑1 (MQ=34) aaCTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg < 1:3137970/71‑1 (MQ=34) aaCTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg > 1:4448339/1‑71 (MQ=34) aaCTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg < 1:6012114/71‑1 (MQ=34) aaCTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCg < 1:1346157/71‑1 (MQ=34) tACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg > 1:358940/1‑70 (MQ=35) tACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCgg > 1:5942885/1‑69 (MQ=35) aCCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCggg > 1:6132928/1‑69 (MQ=35) cAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGt < 1:2206827/71‑1 (MQ=34) ggATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTc > 1:6338468/1‑70 (MQ=35) ggATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTc > 1:1037028/1‑70 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:4717679/52‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:6752560/52‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:919969/52‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:1192888/52‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:1951630/52‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:2083631/52‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:2229981/52‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:2256709/52‑1 (MQ=35) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTa > 1:4562119/1‑71 (MQ=34) gATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTa < 1:4440998/71‑1 (MQ=34) aTCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:3780647/51‑1 (MQ=35) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc > 1:1884785/1‑70 (MQ=35) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc > 1:3839918/1‑70 (MQ=35) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg > 1:3976009/1‑71 (MQ=34) tCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACg > 1:4389025/1‑71 (MQ=34) gcgcCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACgt < 1:6441363/70‑1 (MQ=35) gcgcCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACgt < 1:4563692/70‑1 (MQ=35) gcgcCGTCCATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACgt < 1:1420581/70‑1 (MQ=11) gcgcCGTCCATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACgt < 1:6204939/70‑1 (MQ=11) gcgcCGTCCATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGGCCTGGGCAGTCAACgt < 1:5514204/70‑1 (MQ=11) cgcCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACgt < 1:136568/69‑1 (MQ=35) ccGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGt > 1:2047949/1‑49 (MQ=38) ccGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGc > 1:6018642/1‑71 (MQ=34) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAg < 1:4517093/59‑1 (MQ=35) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAg < 1:3644896/59‑1 (MQ=35) cGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAg < 1:283892/59‑1 (MQ=35) tCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCgg < 1:1776275/43‑1 (MQ=38) tCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCg < 1:351835/42‑1 (MQ=38) tCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTc > 1:6106337/1‑59 (MQ=35) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGcc > 1:2915887/1‑47 (MQ=38) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt > 1:2619866/1‑71 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt > 1:6564143/1‑71 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt > 1:3479845/1‑71 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt > 1:2165152/1‑71 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt > 1:2701333/1‑71 (MQ=34) cATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGt > 1:4500411/1‑71 (MQ=34) cATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGgcc > 1:3641480/1‑48 (MQ=2) cATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGgcc > 1:6080001/1‑48 (MQ=2) cATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGgcc > 1:2428734/1‑48 (MQ=2) cATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGgcc > 1:2632474/1‑48 (MQ=2) cATCTGCGCAGCACCGGTAATCATGTTCTTGATGTAGTCGGCGTGgcc > 1:4271202/1‑48 (MQ=2) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGc > 1:1620691/1‑38 (MQ=40) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGc > 1:4184696/1‑38 (MQ=40) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGcccc > 1:4639724/1‑45 (MQ=38) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc < 1:16282/56‑1 (MQ=35) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc < 1:2273957/56‑1 (MQ=35) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc < 1:4300367/56‑1 (MQ=35) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTAc < 1:2815004/56‑1 (MQ=35) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACgt > 1:632122/1‑58 (MQ=35) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACg > 1:6172737/1‑71 (MQ=34) tGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACg > 1:1313985/1‑71 (MQ=34) cgcAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGa < 1:6288857/67‑1 (MQ=31) | GCCGTCAGTCGCAGCAACTACCAGGATCGCGCCGTCCATCTGCGCAGCACCGGTGATCATGTTTTTAACATAGTCGGCGTGCCCCGGGCAGTCTACGTGTGCGTAGTGACG > minE/2266111‑2266221 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |