Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,432,727 A→G 5.2% I42I (ATT→ATC)  trxA ← thioredoxin 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,432,7270AG 5.2% 287.9 / 5.7 96I42I (ATT→ATC) trxAthioredoxin 1
Reads supporting (aligned to +/- strand):  ref base A (58/33);  new base G (3/2);  total (61/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 101 total aligned reads displayed.

CAGGGTTTTGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGCCCC  >  minE/2432657‑2432789
                                                                      |                                                              
cAGGGTTTTGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGa                                                                <  1:2267454/71‑1 (MQ=255)
 aGGGTTTTGCTCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGaa                                                               >  1:5264070/1‑71 (MQ=255)
 aGGGTTTTGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGaa                                                               >  1:6525387/1‑71 (MQ=255)
 aGGGTTTTGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGaa                                                               >  1:6700526/1‑71 (MQ=255)
 aGGGTTTTGATCGATGTTCAGCTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGaa                                                               >  1:1809159/1‑71 (MQ=255)
   ggTTTTGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATc                                                             <  1:3325181/71‑1 (MQ=255)
     ttttGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATc                                                             <  1:5747719/69‑1 (MQ=255)
     ttttGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCgg                                                           >  1:1386591/1‑71 (MQ=255)
     ttttGATCGATGTTCAGTTTTGCAACGGTCACTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATc                                                             <  1:1892525/69‑1 (MQ=255)
       ttGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCgggg                                                         >  1:3668883/1‑71 (MQ=255)
       ttGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCggg                                                          >  1:3002173/1‑70 (MQ=255)
       ttGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCggg                                                          <  1:1038489/70‑1 (MQ=255)
         gATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGc                                                        >  1:3927222/1‑70 (MQ=255)
         gATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATACAGAATCGGGGc                                                        >  1:6940352/1‑70 (MQ=255)
           tCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGAt                                                     <  1:4200342/71‑1 (MQ=255)
            cGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCggg                                                          >  1:5317706/1‑65 (MQ=255)
            cGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGAt                                                     >  1:4247632/1‑70 (MQ=255)
            cGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGAt                                                     >  1:1460795/1‑70 (MQ=255)
            cGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGAt                                                     >  1:1354712/1‑70 (MQ=255)
              aTGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAt                                                  <  1:6406519/71‑1 (MQ=255)
              aTGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAt                                                  <  1:6212016/71‑1 (MQ=255)
              aTGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAt                                                  <  1:2481638/71‑1 (MQ=255)
               tGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtt                                                 >  1:6409295/1‑71 (MQ=255)
               tGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtt                                                 >  1:1333984/1‑71 (MQ=255)
                 ttCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtttt                                               <  1:4164896/71‑1 (MQ=255)
                   cAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtttt                                               >  1:2881425/1‑69 (MQ=255)
                   cAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTg                                              <  1:4807744/70‑1 (MQ=255)
                   cAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTg                                              <  1:1058218/70‑1 (MQ=255)
                    aGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTg                                              <  1:3234939/69‑1 (MQ=255)
                    aGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTg                                              <  1:1823576/69‑1 (MQ=255)
                      ttttGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACg                                          <  1:2175711/71‑1 (MQ=255)
                       tttGCAACGGTCAGTTTGCGCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACg                                          >  1:3810735/1‑70 (MQ=255)
                       tttGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACg                                          >  1:523211/1‑70 (MQ=255)
                       tttGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACg                                          >  1:2895492/1‑70 (MQ=255)
                           cAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGAcc                                      <  1:4971117/70‑1 (MQ=255)
                             aCGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGca                                   >  1:384752/1‑71 (MQ=255)
                             aCGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGca                                   >  1:4693778/1‑71 (MQ=255)
                             aCGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGca                                   >  1:3594657/1‑71 (MQ=255)
                               ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                  >  1:153735/1‑70 (MQ=255)
                               ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                  >  1:4512591/1‑70 (MQ=255)
                               ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                  >  1:2517074/1‑70 (MQ=255)
                               ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                  >  1:2247126/1‑70 (MQ=255)
                               ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                  >  1:1778734/1‑70 (MQ=255)
                               ggTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcac                                  >  1:1761980/1‑70 (MQ=255)
                                gTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtttt                                               <  1:4519503/56‑1 (MQ=255)
                                 tCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGcaccac                               <  1:1471248/71‑1 (MQ=255)
                                   aGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCActc                             >  1:6797327/1‑71 (MQ=255)
                                   aGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCAct                              <  1:5376043/70‑1 (MQ=255)
                                      ttGCCCTGATATTCGTCAGCGATTTCATCCAGAATCggg                                                          >  1:5811141/1‑39 (MQ=255)
                                       tGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGcc                         >  1:938998/1‑71 (MQ=255)
                                            tGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGa                     >  1:1662793/1‑70 (MQ=255)
                                            tGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGa                     >  1:5139522/1‑70 (MQ=255)
                                            tGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACAACTCTGCCCAGa                     >  1:4557844/1‑70 (MQ=255)
                                             gATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCAtt                                                 <  1:5164318/41‑1 (MQ=255)
                                              atatTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGaaa                   <  1:5482392/70‑1 (MQ=255)
                                              atatTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAAt                  >  1:6322139/1‑71 (MQ=255)
                                               tatTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATc                 <  1:1605802/71‑1 (MQ=255)
                                               tatTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATc                 <  1:1923800/71‑1 (MQ=255)
                                                atTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCTCACCACTCTGCCCAGaa                    <  1:2435066/67‑1 (MQ=255)
                                                 ttCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcga               >  1:6600835/1‑71 (MQ=255)
                                                  tCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcgac              >  1:3516058/1‑71 (MQ=255)
                                                  tCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcgac              >  1:5278249/1‑71 (MQ=255)
                                                   cGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCa                                            >  1:4868466/1‑40 (MQ=255)
                                                   cGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCa                                            >  1:3694484/1‑40 (MQ=255)
                                                   cGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCa                                            >  1:442608/1‑40 (MQ=255)
                                                   cGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCa                                            >  1:645760/1‑40 (MQ=255)
                                                   cGTCAGCGATTTCATCCAGAATCGCGGCGATCATTTTGCa                                            >  1:2991461/1‑40 (MQ=255)
                                                    gTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcgacg             <  1:5412400/70‑1 (MQ=255)
                                                       aGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAgg          <  1:1948317/70‑1 (MQ=255)
                                                       aGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAgg          <  1:5357427/70‑1 (MQ=255)
                                                          gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCg                                     >  1:2152335/1‑40 (MQ=255)
                                                          gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCg                                     >  1:6399522/1‑40 (MQ=255)
                                                          gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAgg          >  1:3402237/1‑67 (MQ=255)
                                                          gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAgg          >  1:1820562/1‑67 (MQ=255)
                                                          gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAgg          >  1:35852/1‑67 (MQ=255)
                                                          gATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCg      <  1:6070039/71‑1 (MQ=255)
                                                           aTTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGc     <  1:3291053/71‑1 (MQ=255)
                                                           aTTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGc     <  1:2532160/71‑1 (MQ=255)
                                                            tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGc                                    >  1:1990091/1‑39 (MQ=255)
                                                            tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGc                                    >  1:6131623/1‑39 (MQ=255)
                                                            tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcc    >  1:1699938/1‑71 (MQ=255)
                                                            tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcc    >  1:3597575/1‑71 (MQ=255)
                                                            tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcc    >  1:5424877/1‑71 (MQ=255)
                                                            tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcc    >  1:3037692/1‑71 (MQ=255)
                                                            tttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcc    >  1:3125715/1‑71 (MQ=255)
                                                             ttcttcCAGGATCGGGGCAATCATCTTGCAAGGACCGcacc                                 <  1:6233763/41‑1 (MQ=19)
                                                             ttcttcCAGGATCGGGGCAATCATCTTGCAAGGACCGcacc                                 <  1:3396147/41‑1 (MQ=19)
                                                             ttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcga               >  1:6500999/1‑59 (MQ=255)
                                                             ttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGccc   >  1:6837776/1‑71 (MQ=255)
                                                             ttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGccc   >  1:410147/1‑71 (MQ=255)
                                                             ttCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGccc   >  1:161159/1‑71 (MQ=255)
                                                               cttCCAGGATCGGGGCAATCATCTTGCAAGGACCGcaccac                               >  1:1499214/3‑41 (MQ=19)
                                                               cttCCAGGATCGGGGCAATCATCTTGCAAGGACCGcaccac                               >  1:6848461/3‑41 (MQ=19)
                                                               cttCCAGGATCGGGGCAATCATCTTGCAAGGACCGcaccac                               >  1:1665947/3‑41 (MQ=19)
                                                               cATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcccc  >  1:3450008/1‑70 (MQ=255)
                                                               cATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcccc  <  1:6783380/70‑1 (MQ=255)
                                                               cATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGcccc  <  1:1672537/70‑1 (MQ=255)
                                                                aTCCAGAATCGTGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcga               <  1:1533356/56‑1 (MQ=255)
                                                                aTCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATcga               <  1:3649687/56‑1 (MQ=255)
                                                                    aGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAg           >  1:2097998/1‑56 (MQ=255)
                                                                      |                                                              
CAGGGTTTTGATCGATGTTCAGTTTTGCAACGGTCAGTTTGCCCTGATATTCGTCAGCGATTTCATCCAGAATCGGGGCGATCATTTTGCACGGACCGCACCACTCTGCCCAGAAATCGACGAGGATCGCCCC  >  minE/2432657‑2432789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑