| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,494,963 | C→T | 7.4% | intergenic (+17/‑125) | ibpB → / → yidF | heat shock chaperone/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,494,963 | 0 | C | T | 7.4% | 351.1 / 13.3 | 109 | intergenic (+17/‑125) | ibpB/yidF | heat shock chaperone/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (56/44); new base T (6/2); total (63/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
Only 100 of 113 total aligned reads displayed.
CATCGCAGCGCAGCGTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGTTTAA > minE/2494895‑2495030 | cATCGCAGCGCAGCGTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGccc > 1:6475970/1‑71 (MQ=255) agcgcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt < 1:759323/71‑1 (MQ=255) agcgcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt < 1:51318/71‑1 (MQ=255) agcgcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt < 1:5904388/71‑1 (MQ=255) cgcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTc < 1:5721940/71‑1 (MQ=255) gcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTc < 1:4276987/70‑1 (MQ=255) gcagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCt > 1:1827187/1‑71 (MQ=255) cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg > 1:2912738/1‑71 (MQ=255) cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg > 1:5349078/1‑71 (MQ=255) cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg < 1:1825029/71‑1 (MQ=255) cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg < 1:5400249/71‑1 (MQ=255) cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg < 1:1570800/71‑1 (MQ=255) cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg < 1:5848296/71‑1 (MQ=255) cagcgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg < 1:6430225/71‑1 (MQ=255) cgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtggtg > 1:537489/1‑71 (MQ=255) cgTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCCCCATTCtggtg > 1:3839563/1‑71 (MQ=255) tCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCa > 1:6226721/1‑59 (MQ=255) tCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCa > 1:4961241/1‑59 (MQ=255) tCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCa > 1:4500740/1‑59 (MQ=255) cGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGcc < 1:6221940/57‑1 (MQ=255) cGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGcc < 1:6322925/57‑1 (MQ=255) cGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGcc < 1:4212972/57‑1 (MQ=255) cTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCttt > 1:1634152/1‑71 (MQ=255) cTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCttt > 1:5449324/1‑71 (MQ=255) cTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCttt > 1:3099440/1‑71 (MQ=255) tATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCtttt > 1:4208789/1‑71 (MQ=255) tCAGCGGACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGACATTCTGGTGGGGCtttttt > 1:5221/1‑71 (MQ=255) gAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGc > 1:1813092/1‑41 (MQ=255) gAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGc > 1:1661176/1‑41 (MQ=255) gAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGc > 1:1379446/1‑41 (MQ=255) aaCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTct > 1:475217/1‑70 (MQ=255) aaCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTct > 1:4524106/1‑70 (MQ=255) aaCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTct > 1:4288952/1‑70 (MQ=255) aaCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTct > 1:2546769/1‑70 (MQ=255) aaCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTct > 1:4071682/1‑70 (MQ=255) aCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtg < 1:3296989/51‑1 (MQ=255) aCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTctta < 1:5924301/71‑1 (MQ=255) aCGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTctta < 1:4017666/71‑1 (MQ=255) cGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtggtg > 1:6660923/1‑53 (MQ=255) cGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtggtg > 1:2292277/1‑53 (MQ=255) cGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCtggtg > 1:3513719/1‑53 (MQ=255) cGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttac > 1:2087339/1‑71 (MQ=255) gTCCAGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttact < 1:4902753/71‑1 (MQ=255) cccGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCtttt < 1:5001250/58‑1 (MQ=255) cccGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCtttt < 1:4171463/58‑1 (MQ=255) gcgTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTtacttact > 1:1689531/1‑70 (MQ=255) gcgTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTtacttact > 1:6037723/1‑70 (MQ=255) gcgTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTtacttact > 1:4607143/1‑70 (MQ=255) gcgTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTtacttact > 1:3153008/1‑70 (MQ=255) cgTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTca < 1:513111/71‑1 (MQ=255) ttAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt < 1:3834708/37‑1 (MQ=255) ttAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt < 1:1988624/37‑1 (MQ=255) ttAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCAt < 1:5787307/37‑1 (MQ=255) aaaTAGCTAACTAGCTATTCTCTTTGCCCCGCCATTc > 1:2366238/1‑37 (MQ=255) aaTAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTcaacaa < 1:1170927/70‑1 (MQ=255) aTAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATg < 1:4183975/71‑1 (MQ=255) tAGCTAACTAGCTATTCTCTTTGTCCCGCCATTCtg < 1:5778669/36‑1 (MQ=255) tAGCTAACTAGCTATTCTCTTTGTCCCGCCATTCtg < 1:6232719/36‑1 (MQ=255) gCTAACTAGCTATTCTCTTTGTCCCGCCATTCtggt > 1:5429251/1‑36 (MQ=255) gCTAACTAGCTATTCTCTTTGTCCCGCCATTCtggt > 1:6395765/1‑36 (MQ=255) gCTAACTAGCTATTCTCTTTGTCCCGCCATTCtggt > 1:505199/1‑36 (MQ=255) gCTAACTAGCTATTCTCTTTGTCCCGCCATTCtggt > 1:3310108/1‑36 (MQ=255) gCTAACTAGCTATTCTCTTTGTCCCGCCATTCtggt > 1:4904037/1‑36 (MQ=255) cTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactta > 1:5212998/1‑57 (MQ=255) cTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactta > 1:2829380/1‑57 (MQ=255) aCTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactta < 1:1975227/54‑1 (MQ=255) aCTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTaaa > 1:3168883/1‑71 (MQ=255) aCTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTaaa > 1:1890366/1‑71 (MQ=255) tAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactt > 1:6518084/1‑51 (MQ=255) tAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactt > 1:2658257/1‑51 (MQ=255) tAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTtacttact > 1:4121727/1‑54 (MQ=255) tAGCTATGCTCTTTGCCCCGCCATTCTGGGGGGGCTTTTTTTGTctta < 1:2442992/48‑1 (MQ=38) gCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTc < 1:2772245/71‑1 (MQ=255) gCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTc > 1:1154768/1‑71 (MQ=255) gCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTc < 1:1011267/71‑1 (MQ=255) cTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTc > 1:5618896/1‑70 (MQ=255) ttCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTcaac < 1:6373689/52‑1 (MQ=255) ttCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACt > 1:4712718/1‑71 (MQ=255) ttCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACt > 1:1467589/1‑71 (MQ=255) tctcTTTGCCCCGCCATTCTGGTGGGGCttttttt < 1:3018298/35‑1 (MQ=255) ctctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTc > 1:1096929/1‑47 (MQ=255) ctctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGt > 1:722976/1‑71 (MQ=255) ctctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGt > 1:2683673/1‑71 (MQ=255) ctctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGt > 1:138295/1‑71 (MQ=255) ctctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGt > 1:2821715/1‑71 (MQ=255) ctctTTGCCCCGCCATTCTGGTGGGGCGTTTTTTGTCTTACTTACTc > 1:4530945/1‑47 (MQ=255) tctTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGtt > 1:3467213/1‑71 (MQ=255) tctTTGACCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGtt > 1:11917/1‑71 (MQ=255) tttGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTaa > 1:6473975/1‑57 (MQ=255) tttGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGttt > 1:3133536/1‑70 (MQ=255) tttGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGttt > 1:525764/1‑70 (MQ=255) tttGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGt < 1:3406764/68‑1 (MQ=255) tttGCCACGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGtt > 1:1854/1‑69 (MQ=255) ttGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTg < 1:3019747/66‑1 (MQ=255) tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTcttactta > 1:1036428/1‑39 (MQ=255) tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGttt < 1:5958005/68‑1 (MQ=255) tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGttt < 1:5862515/68‑1 (MQ=255) tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGtt > 1:4051131/1‑67 (MQ=255) tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGTTTaa < 1:5950166/70‑1 (MQ=255) tGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGTTTaa < 1:6574581/70‑1 (MQ=255) | CATCGCAGCGCAGCGTATCGCTATCAGCGAACGTCCCGCGTTAAATAGCTAACTAGCTATTCTCTTTGCCCCGCCATTCTGGTGGGGCTTTTTTTGTCTTACTTACTCAACAATGCGTTAAATTTCGACTGTTTAA > minE/2494895‑2495030 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |