| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,736,245 | A→G | 5.4% | E463E (GAA→GAG) | fusA → | protein chain elongation factor EF‑G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,736,245 | 0 | A | G | 5.4% | 555.3 / 16.3 | 185 | E463E (GAA→GAG) | fusA | protein chain elongation factor EF‑G |
| Reads supporting (aligned to +/- strand): ref base A (86/89); new base G (4/6); total (90/95) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 189 total aligned reads displayed.
AGAAGACCCGTCTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGC > minE/2736176‑2736315 | ccGTCTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACt < 1:460267/65‑1 (MQ=255) ccGTCTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACt < 1:3049168/65‑1 (MQ=255) gTCTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCg < 1:4812376/71‑1 (MQ=255) cTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGa > 1:1005790/1‑70 (MQ=255) cTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGa > 1:4081801/1‑70 (MQ=255) cTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGa > 1:5913978/1‑70 (MQ=255) tttCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAca > 1:1631500/1‑71 (MQ=255) tttCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAca > 1:6886103/1‑71 (MQ=255) tttCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGAGTATGGGCGAACTGCACCTCGAca > 1:1825642/1‑71 (MQ=255) tttCCGTGTATGGACAGACGAAGAATCTAACCAGACCATCATCGCGGGTATCGGCGAACTGCACCTCGAc > 1:2249658/1‑70 (MQ=255) tCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCTCGGGTATGGGCGAACTGCACCTCGAcat < 1:3787074/70‑1 (MQ=255) tCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCTAcat < 1:4642228/70‑1 (MQ=255) tCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAcat < 1:6523327/70‑1 (MQ=255) tgtATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCg < 1:5225114/71‑1 (MQ=255) tATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGc < 1:3856332/54‑1 (MQ=255) tATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGc < 1:2036896/54‑1 (MQ=255) tATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGtt > 1:1920943/1‑71 (MQ=255) tGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGa > 1:4468353/1‑71 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCTGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATg < 1:739670/71‑1 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAcatcat < 1:6404398/58‑1 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt > 1:628054/1‑70 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATg < 1:3796661/71‑1 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATg < 1:2660561/71‑1 (MQ=255) gaagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGaa < 1:6709180/71‑1 (MQ=255) aagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGaa > 1:5876865/1‑70 (MQ=255) aagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAg > 1:2238149/1‑71 (MQ=255) gaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCg > 1:5878672/1‑71 (MQ=255) aTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTg < 1:4693833/71‑1 (MQ=255) tAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGaa > 1:5299489/1‑70 (MQ=255) aCCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTc < 1:1333472/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:1388362/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:6504841/71‑1 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGAcatc > 1:6436195/1‑37 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGAcatc > 1:4203852/1‑37 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGtt > 1:6341445/1‑70 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGtt > 1:6349278/1‑70 (MQ=255) atcGCGGGTATGGGCGAACTTCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:2174069/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:4710064/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:4956641/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:5525506/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:101987/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:934541/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:6907932/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:426443/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:1516395/1‑71 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:3082801/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:661245/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg < 1:177773/66‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg < 1:3731883/66‑1 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:4982906/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:5120762/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:242732/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:4067725/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa < 1:4530414/70‑1 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa < 1:5370392/70‑1 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa < 1:3426656/70‑1 (MQ=255) ggTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:3927498/56‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:6473523/40‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:5248998/40‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:4088707/40‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:3682401/40‑1 (MQ=255) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:2868414/39‑1 (MQ=16) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:4345253/39‑1 (MQ=16) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:1809811/39‑1 (MQ=16) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:3215305/39‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGCATTCaa < 1:887262/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:280072/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:6637131/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:564299/54‑1 (MQ=255) aTGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCATGAAGCGTGAGTTCAACGTGGaa > 1:5956996/1‑60 (MQ=255) aTGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:6780166/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:6208337/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:4283245/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:881510/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:2295665/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:3339798/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:4121012/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:6114597/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:3127245/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:5683570/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:5715923/70‑1 (MQ=255) gggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAATCGAACGTAgg < 1:362588/69‑1 (MQ=255) gggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:2344979/69‑1 (MQ=255) ggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt > 1:6550505/1‑53 (MQ=255) ggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt > 1:795061/1‑53 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:4832772/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:517549/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:2446872/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:5489715/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:3496298/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg < 1:4254432/54‑1 (MQ=255) cGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAc > 1:3319087/1‑71 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:1278170/1‑55 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:3053469/1‑55 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:4413281/1‑55 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:1841010/1‑55 (MQ=255) aaCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCg > 1:811120/1‑71 (MQ=255) aCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGc < 1:6628493/71‑1 (MQ=255) aCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGc < 1:3722346/71‑1 (MQ=255) aCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGc < 1:4664437/71‑1 (MQ=255) | AGAAGACCCGTCTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGC > minE/2736176‑2736315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |