| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,736,254 | C→G | 5.1% | L466L (CTC→CTG) | fusA → | protein chain elongation factor EF‑G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,736,254 | 0 | C | G | 5.1% | 667.8 / 14.6 | 194 | L466L (CTC→CTG) | fusA | protein chain elongation factor EF‑G |
| Reads supporting (aligned to +/- strand): ref base C (90/94); new base G (4/6); total (94/100) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 198 total aligned reads displayed.
GTCTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTG > minE/2736185‑2736321 | cTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGa > 1:5913978/1‑70 (MQ=255) cTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGa > 1:1005790/1‑70 (MQ=255) cTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGa > 1:4081801/1‑70 (MQ=255) cTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGa > 1:5336841/1‑70 (MQ=255) tttCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAca > 1:2248543/1‑71 (MQ=255) tttCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAca > 1:6886103/1‑71 (MQ=255) tttCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAca > 1:1631500/1‑71 (MQ=255) tCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCTAcat < 1:4642228/70‑1 (MQ=255) tCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAcat < 1:6652085/70‑1 (MQ=255) tCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAcat < 1:6523327/70‑1 (MQ=255) tCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAcat < 1:4811932/70‑1 (MQ=255) cGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGAcatcat > 1:5566613/1‑71 (MQ=255) tATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGtt > 1:2370818/1‑71 (MQ=255) tATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGtt > 1:5814641/1‑71 (MQ=255) aTGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGtt > 1:6363456/1‑70 (MQ=255) aTGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGtt > 1:3735176/1‑70 (MQ=255) tGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGa > 1:4468353/1‑71 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCTGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATg < 1:739670/71‑1 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt > 1:2326900/1‑70 (MQ=255) aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATg < 1:3796661/71‑1 (MQ=255) gaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCg > 1:5878672/1‑71 (MQ=255) tAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGaa > 1:6344069/1‑70 (MQ=255) tAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGaa > 1:5299489/1‑70 (MQ=255) tAACCAGACCATCATCGCGGGTATGGGCGAACTGCACATCGACATCATCGTTGACCGTATGAAGCGTGa > 1:2949134/1‑69 (MQ=255) aCCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTc < 1:6632066/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:6504841/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:1388362/71‑1 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGAcatc > 1:4203852/1‑37 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGAcatc > 1:6436195/1‑37 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg < 1:4299652/71‑1 (MQ=255) atcGCGGGTATGGGCGAACTTCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:2174069/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:101987/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:4956641/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:549682/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:2731120/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:6907932/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:934541/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:1516395/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:1823228/1‑71 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:5673905/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:4707654/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:3082801/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg < 1:177773/66‑1 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:3488603/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:4982906/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:5210485/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:5699206/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa < 1:2824300/70‑1 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa < 1:4530414/70‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:5248998/40‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:4088707/40‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:6473523/40‑1 (MQ=255) gTATGGGCGAACCGCACCTCGACATCATCGTTGACCGTAt < 1:3364622/40‑1 (MQ=255) tATTGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:2272030/54‑1 (MQ=255) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:1294041/39‑1 (MQ=16) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:3327738/39‑1 (MQ=16) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:3215305/39‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGCATTCaa < 1:887262/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:3688840/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAg > 1:3520655/1‑71 (MQ=255) aTGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCATGAAGCGTGAGTTCAACGTGGaa > 1:5956996/1‑60 (MQ=255) aTGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCATGAAGCGTGAGTTCAACGTGGaa > 1:6619779/1‑60 (MQ=255) aTGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCATGAAGCGTGAGTTCAACGTGGaa > 1:372499/1‑60 (MQ=255) aTGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:6780166/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:881510/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:4054594/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:4121012/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:6114597/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:1969056/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:3127245/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:4278782/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg > 1:4364388/1‑70 (MQ=255) tGGGCGAACTGCACCTCAACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg > 1:392734/1‑70 (MQ=255) gggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAATCGAACGTAgg < 1:362588/69‑1 (MQ=255) ggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt > 1:6550505/1‑53 (MQ=255) ggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt > 1:1187896/1‑53 (MQ=255) ggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt > 1:795061/1‑53 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:2446872/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:5413812/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:4832772/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg < 1:4254432/54‑1 (MQ=255) cGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAc > 1:622032/1‑71 (MQ=255) cGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAc > 1:6287149/1‑71 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:3053469/1‑55 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:1841010/1‑55 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:5134594/1‑55 (MQ=255) aaCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCg > 1:811120/1‑71 (MQ=255) aCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGc < 1:4664437/71‑1 (MQ=255) cTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAcc > 1:6183520/1‑68 (MQ=255) cTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCa < 1:5076167/71‑1 (MQ=255) tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt < 1:1614313/46‑1 (MQ=255) tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt < 1:4463348/46‑1 (MQ=255) tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt < 1:3453177/46‑1 (MQ=255) gCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAgg > 1:4607324/1‑71 (MQ=255) cACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGt < 1:1335273/71‑1 (MQ=255) cACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGt < 1:2233858/71‑1 (MQ=255) ccTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTg < 1:1888268/71‑1 (MQ=255) cGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTaaa > 1:329815/1‑58 (MQ=255) cGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTaaa > 1:2638174/1‑58 (MQ=255) cGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTaaa > 1:4440148/1‑58 (MQ=255) | GTCTTTCCGTGTATGGACTGACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTG > minE/2736185‑2736321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |