| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,736,272 | T→C | 5.0% | R472R (CGT→CGC) | fusA → | protein chain elongation factor EF‑G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,736,272 | 0 | T | C | 5.0% | 615.8 / 16.7 | 199 | R472R (CGT→CGC) | fusA | protein chain elongation factor EF‑G |
| Reads supporting (aligned to +/- strand): ref base T (84/105); new base C (4/6); total (88/111) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
Only 100 of 203 total aligned reads displayed.
ACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAACTATCCGC > minE/2736205‑2736341 | aCGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATg < 1:3796661/71‑1 (MQ=255) gaagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGaa < 1:6709180/71‑1 (MQ=255) aagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGaa > 1:5876865/1‑70 (MQ=255) aagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAg > 1:4082267/1‑71 (MQ=255) aagaaTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAg > 1:2238149/1‑71 (MQ=255) aTCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTg < 1:4693833/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:6506416/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:1388362/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:4957974/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:4937805/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:2469836/71‑1 (MQ=255) cAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:2226066/71‑1 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGtt > 1:6349278/1‑70 (MQ=255) catcatCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGtt > 1:6341445/1‑70 (MQ=255) tcatcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg > 1:6543640/1‑69 (MQ=255) catcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:6841090/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:101987/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:4710064/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:2731120/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:549682/1‑62 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:1823228/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:6907932/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:426443/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:1516395/1‑71 (MQ=255) atcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc > 1:934541/1‑71 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:4227830/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:3082801/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGt < 1:661245/53‑1 (MQ=255) tcGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTg < 1:177773/66‑1 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:4982906/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:5041131/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:3488603/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:5120762/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGaa > 1:242732/1‑71 (MQ=255) gcgGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGa < 1:4530414/70‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:6473523/40‑1 (MQ=255) gTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTAt < 1:5248998/40‑1 (MQ=255) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:1294041/39‑1 (MQ=16) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:1479535/39‑1 (MQ=16) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:4345253/39‑1 (MQ=16) tATGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCAt < 1:2868414/39‑1 (MQ=16) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGCATTCaa < 1:887262/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:564299/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:589403/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:6637131/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:859970/54‑1 (MQ=255) tATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa < 1:3688840/54‑1 (MQ=255) aTGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCATGAAGCGTGAGTTCAACGTGGaa > 1:5956996/1‑60 (MQ=255) aTGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCATGAAGCGTGAGTTCAACGTGGaa > 1:372499/1‑60 (MQ=255) aTGGGCGAGCTGCACCTGGAAATTATCGTTGACCGCATGAAGCGTGAGTTCAACGTGGaa > 1:796882/1‑60 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:4054594/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:4283245/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAAc < 1:761089/53‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:3339798/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:1969056/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:5683570/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:5715923/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:2295665/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:3127245/70‑1 (MQ=255) tGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg < 1:6114597/70‑1 (MQ=255) tGGGCGAACTGCACCTCAACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAgg > 1:392734/1‑70 (MQ=255) gggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAATCGAACGTAgg < 1:362588/69‑1 (MQ=255) ggCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt > 1:1187896/1‑53 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:4832772/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:5413812/1‑49 (MQ=255) gCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCaa > 1:4652862/1‑49 (MQ=255) cGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAc > 1:6287149/1‑71 (MQ=255) cGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAc > 1:622032/1‑71 (MQ=255) cGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAc > 1:3319087/1‑71 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:4413281/1‑55 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAg > 1:3053469/1‑55 (MQ=255) gAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGc < 1:4104792/56‑1 (MQ=255) aCTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGc < 1:6628493/71‑1 (MQ=255) cTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAAcc > 1:6183520/1‑68 (MQ=255) tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt < 1:4463348/46‑1 (MQ=255) tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt < 1:3453177/46‑1 (MQ=255) tGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGt < 1:1614313/46‑1 (MQ=255) gCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAgg > 1:4607324/1‑71 (MQ=255) cACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGt < 1:3285452/71‑1 (MQ=255) cACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGt < 1:1335273/71‑1 (MQ=255) ccTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTg < 1:3570995/71‑1 (MQ=255) ccTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTg < 1:1888268/71‑1 (MQ=255) cGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTaaa > 1:5331688/1‑58 (MQ=255) cGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTaaa > 1:2638174/1‑58 (MQ=255) gACATCATCGTTGACCGTATGAAGCGTGAATTCAACTTTGAAGCGAACGTAGGTAAACCGCAGGTTGCt < 1:4486772/69‑1 (MQ=255) gACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCt < 1:6065206/69‑1 (MQ=255) gACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCt < 1:1704167/69‑1 (MQ=255) gACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTa > 1:2967402/1‑71 (MQ=255) aCATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTAc < 1:1770314/71‑1 (MQ=255) aCATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTAc < 1:6954121/71‑1 (MQ=255) catcatCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTAcc > 1:5454845/1‑71 (MQ=255) atcGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTaaa > 1:2340624/1‑51 (MQ=255) atcGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTg < 1:5177285/70‑1 (MQ=255) atcGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGa < 1:6851514/71‑1 (MQ=255) atcGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGa < 1:5140209/71‑1 (MQ=255) atcGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGa < 1:3653874/71‑1 (MQ=255) gTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAAc < 1:2803320/71‑1 (MQ=255) tGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAACTa > 1:738622/1‑71 (MQ=255) gTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAACTATCCGc < 1:4254356/71‑1 (MQ=255) tATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCg < 1:3414116/43‑1 (MQ=255) | ACGAAGAATCTAACCAGACCATCATCGCGGGTATGGGCGAACTGCACCTCGACATCATCGTTGACCGTATGAAGCGTGAATTCAACGTTGAAGCGAACGTAGGTAAACCGCAGGTTGCTTACCGTGAAACTATCCGC > minE/2736205‑2736341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |