| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 72,621 | A→G | 7.1% | intergenic (+33/‑147) | ilvH → / → fruR | acetolactate synthase III, thiamin‑dependent, small subunit/DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 72,621 | 0 | A | G | 7.1% | 331.0 / 13.8 | 112 | intergenic (+33/‑147) | ilvH/fruR | acetolactate synthase III, thiamin‑dependent, small subunit/DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base A (60/44); new base G (3/5); total (63/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.94e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.46e-01 | |||||||||||
Only 100 of 114 total aligned reads displayed.
GTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTT > minE/72553‑72690 | gTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATg > 1:6115381/1‑71 (MQ=255) ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCa > 1:799170/1‑71 (MQ=255) ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCa > 1:3610645/1‑71 (MQ=255) ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCa > 1:2050186/1‑71 (MQ=255) ggACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCa > 1:6503920/1‑71 (MQ=255) gACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCAc > 1:2425725/1‑71 (MQ=255) tttCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGtt > 1:3494344/1‑71 (MQ=255) tttCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGt > 1:3704254/1‑70 (MQ=255) ttCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTg > 1:5623721/1‑71 (MQ=255) ttCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTg > 1:2163870/1‑71 (MQ=255) ttCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTg > 1:5688387/1‑71 (MQ=255) tctcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGtt < 1:5583293/66‑1 (MQ=255) tCGCGCGGCGATAAATTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg > 1:90587/1‑71 (MQ=255) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTgg > 1:2660493/1‑71 (MQ=39) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTgg > 1:2654392/1‑71 (MQ=39) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTgg > 1:6859709/1‑71 (MQ=39) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTg < 1:4596965/70‑1 (MQ=255) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACGTGACATGTTg < 1:5201108/70‑1 (MQ=255) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg > 1:5817214/1‑71 (MQ=255) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg > 1:3110159/1‑71 (MQ=255) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg > 1:291667/1‑71 (MQ=255) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg > 1:5197691/1‑71 (MQ=255) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg > 1:5478367/1‑71 (MQ=255) tCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg > 1:1483168/1‑71 (MQ=255) cgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTggg > 1:1668766/1‑71 (MQ=255) cgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg > 1:2248727/1‑70 (MQ=255) cgcgcgGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTgg < 1:2654339/70‑1 (MQ=255) gcggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtt > 1:2247009/1‑71 (MQ=255) gcggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtt > 1:4966138/1‑71 (MQ=255) gcggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtt > 1:2977509/1‑71 (MQ=255) cggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCttt < 1:1436254/71‑1 (MQ=255) ggctATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttt < 1:5108237/71‑1 (MQ=255) ggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttt < 1:3624004/71‑1 (MQ=255) ggcgATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttt < 1:1588105/71‑1 (MQ=255) aTAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCttt < 1:435013/66‑1 (MQ=255) aTAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTg < 1:5773553/71‑1 (MQ=255) aaaaTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttttttt > 1:2391555/1‑68 (MQ=255) aaaaTAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCttttt > 1:1715819/1‑66 (MQ=255) tataatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTg < 1:2867987/66‑1 (MQ=255) aataatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa < 1:1723324/71‑1 (MQ=255) aataatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa < 1:3263784/71‑1 (MQ=255) aataatGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa < 1:1399489/71‑1 (MQ=255) atGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc < 1:4901127/70‑1 (MQ=255) atGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc < 1:5103782/70‑1 (MQ=255) atGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc < 1:1442562/70‑1 (MQ=255) cGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTg > 1:5621637/1‑71 (MQ=255) ttGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTg < 1:5380288/58‑1 (MQ=255) ttGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCa < 1:4760389/66‑1 (MQ=255) gagaATGATCTCAATGCGCAATTTACAGCCCAACa > 1:1243366/1‑35 (MQ=255) gagaATGATCTCAATGCGCAATTTACAGCCCAACa > 1:2560617/1‑35 (MQ=255) gagaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACg > 1:2716365/1‑42 (MQ=255) gagaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGaa > 1:3586377/1‑71 (MQ=255) gagaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGa < 1:5333660/70‑1 (MQ=255) agaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGaa < 1:619469/70‑1 (MQ=255) agaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGaa < 1:4938321/70‑1 (MQ=255) gaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAAcc > 1:1834400/1‑71 (MQ=255) gaATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAAcc > 1:1903542/1‑71 (MQ=255) aaTGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCt > 1:5974356/1‑71 (MQ=255) tGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa > 1:2555692/1‑55 (MQ=255) tGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa > 1:6120125/1‑55 (MQ=255) tGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa > 1:4699228/1‑55 (MQ=255) tGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTgg < 1:1119855/71‑1 (MQ=255) aTCTCAATGCGCAATTTACAGCCCAACATGTCACg > 1:1944828/1‑35 (MQ=255) aTCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAg > 1:6722816/1‑58 (MQ=255) tcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttt < 1:2204750/42‑1 (MQ=255) tcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCtttt < 1:2766466/42‑1 (MQ=255) tcAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATa > 1:3415210/1‑70 (MQ=255) cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa < 1:2914986/49‑1 (MQ=255) cAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGaa < 1:3281054/49‑1 (MQ=255) aaTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaa > 1:3297294/1‑69 (MQ=255) aaTGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaa < 1:6014863/69‑1 (MQ=255) tGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGc < 1:3999243/71‑1 (MQ=255) gcgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGa > 1:3486480/1‑55 (MQ=255) gcgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAg > 1:292348/1‑69 (MQ=255) cgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaa > 1:6405240/1‑65 (MQ=255) cgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaa > 1:237778/1‑65 (MQ=255) cgcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATaa > 1:2374651/1‑65 (MQ=255) gcAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGt < 1:4772393/71‑1 (MQ=255) cAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAAt < 1:3418989/61‑1 (MQ=255) aTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc < 1:3776421/43‑1 (MQ=255) aTTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATc < 1:2807195/43‑1 (MQ=255) tttACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGcc < 1:3075776/71‑1 (MQ=255) tACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTgg > 1:2062554/1‑45 (MQ=255) tACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTgg > 1:3757725/1‑45 (MQ=255) tACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTgg > 1:1264868/1‑45 (MQ=255) tACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTgg > 1:6839979/1‑45 (MQ=255) tACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCg > 1:502394/1‑70 (MQ=255) aCAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGc < 1:6257750/70‑1 (MQ=255) cAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGc < 1:1444594/69‑1 (MQ=255) cAGCCCAACATGTCACGTGGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCa < 1:5616824/70‑1 (MQ=255) ccAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTaa < 1:6241977/71‑1 (MQ=255) ccAACATGTCACGTTGGGCTTTTTTTCCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTaa < 1:648277/71‑1 (MQ=255) cAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAAt < 1:2698488/49‑1 (MQ=255) cAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAAt > 1:2618608/1‑71 (MQ=255) aCATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAAttt > 1:4655809/1‑71 (MQ=255) cATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGt > 1:6782669/1‑55 (MQ=255) cATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGt > 1:1839336/1‑55 (MQ=255) cATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGt > 1:716201/1‑55 (MQ=255) cATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGt > 1:5204310/1‑55 (MQ=255) cATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAAtttt < 1:6283971/71‑1 (MQ=255) | GTCGGACTTTCGCGCGGCGATAAAATAATGCGTTGAGAATGATCTCAATGCGCAATTTACAGCCCAACATGTCACGTTGGGCTTTTTTTGCGAAATCAGTGGGAACCTGGAATAAAAGCAGTTGCCGCAGTTAATTTT > minE/72553‑72690 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |