| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,827,243 | G→C | 8.4% | T336S (ACT→AGT) | dcuA ← | C4‑dicarboxylate antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,827,243 | 0 | G | C | 8.4% | 257.1 / 10.7 | 83 | T336S (ACT→AGT) | dcuA | C4‑dicarboxylate antiporter |
| Reads supporting (aligned to +/- strand): ref base G (42/34); new base C (2/5); total (44/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGAC > minE/2827173‑2827311 | ggCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGgtag > 1:6080266/1‑71 (MQ=255) cAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTAc > 1:6943326/1‑71 (MQ=255) cAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTAc > 1:6164619/1‑71 (MQ=255) aGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTAcc > 1:6244094/1‑71 (MQ=255) aGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTAc > 1:2823152/1‑70 (MQ=255) cAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCgtg > 1:3720148/1‑71 (MQ=255) acacCCAGAGTACTCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTc < 1:3201427/71‑1 (MQ=255) cacCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTca > 1:3033755/1‑71 (MQ=255) cccAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatc > 1:375559/1‑71 (MQ=255) ccAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcc < 1:221193/71‑1 (MQ=255) ccAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcc < 1:6695320/71‑1 (MQ=255) ccAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatc > 1:6542400/1‑70 (MQ=255) cAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcca > 1:191210/1‑71 (MQ=255) agagTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatccat < 1:3328924/71‑1 (MQ=255) agagTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatccat > 1:4533535/1‑71 (MQ=255) agagTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatccat < 1:135761/71‑1 (MQ=255) agagTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatccat < 1:5964968/71‑1 (MQ=255) gagTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatccatc > 1:16525/1‑71 (MQ=255) agTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCt < 1:1184431/71‑1 (MQ=255) tACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGt < 1:5745667/71‑1 (MQ=255) tACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGt < 1:4284432/71‑1 (MQ=255) cccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTAc > 1:2725868/1‑71 (MQ=255) cccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTAc > 1:2363966/1‑71 (MQ=255) cccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTAc < 1:4093729/71‑1 (MQ=255) cccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTAc > 1:5405553/1‑71 (MQ=255) ccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTACTACCCgta < 1:2634354/56‑2 (MQ=255) ccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTACTACCCgta < 1:427106/56‑2 (MQ=255) ccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTACTACCCgta < 1:4373290/56‑2 (MQ=255) ccGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTACTACCCgta < 1:2772942/56‑2 (MQ=255) gggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGgtagta > 1:3369252/1‑48 (MQ=255) gggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGgtagta > 1:5274852/1‑48 (MQ=255) gggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGgtagta > 1:6674785/1‑48 (MQ=255) gggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGgtagta > 1:569924/1‑48 (MQ=255) gggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTACTACCCGTATc > 1:1741793/1‑56 (MQ=39) gggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTACTACCCGTATc > 1:2236032/1‑56 (MQ=39) ggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCgca < 1:6280326/71‑1 (MQ=255) ggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCg > 1:5354851/1‑69 (MQ=255) ggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCg > 1:6756040/1‑69 (MQ=255) ggATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCg > 1:4352149/1‑69 (MQ=255) aTGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatccat > 1:822350/1‑59 (MQ=255) aagaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCaa < 1:5835898/70‑1 (MQ=255) agaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCgcagca < 1:2570815/68‑1 (MQ=255) agaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCgcagca < 1:5749502/68‑1 (MQ=255) agaaCGGATGGTTGAAGACGAATTTACCGATACGGGTACTACCCGTGTCATCCATCTGTACCgcagca < 1:5549570/68‑1 (MQ=255) gaaCGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAAc > 1:669678/1‑69 (MQ=255) ggATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGt < 1:5879783/71‑1 (MQ=255) aTGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCg < 1:6514248/71‑1 (MQ=255) tGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatccatc < 1:4636392/48‑1 (MQ=255) tGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatccatc < 1:4135658/48‑1 (MQ=255) tGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatccatc < 1:1403403/48‑1 (MQ=255) tGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatccatc < 1:6866224/48‑1 (MQ=255) tGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCg < 1:6635822/67‑1 (MQ=255) ggTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAAc > 1:2641647/1‑61 (MQ=255) ggTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAAc > 1:6037197/1‑61 (MQ=255) gTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTc > 1:6832249/1‑67 (MQ=255) gTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTc > 1:6096580/1‑67 (MQ=255) gTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTc > 1:6062801/1‑67 (MQ=255) ttGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCggg > 1:737204/1‑69 (MQ=255) tGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcc < 1:6297249/41‑1 (MQ=255) tGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcc < 1:767997/41‑1 (MQ=255) tGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcc < 1:3929346/41‑1 (MQ=255) tGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcc < 1:2822783/41‑1 (MQ=255) tGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcc < 1:5552169/41‑1 (MQ=255) tGAAGACGAATTTACCGATACGGGTAGTACCCGTGTcatcc < 1:3416326/41‑1 (MQ=255) gAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTa > 1:1339401/1‑69 (MQ=255) gAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTa > 1:2747836/1‑69 (MQ=255) gAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTa > 1:6822862/1‑69 (MQ=255) aaGACGAATTTACCGATACGGGTAGTACCCGTGTcatccat > 1:2131252/1‑41 (MQ=255) aGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCTTCTGTACCGCAGCAACCa > 1:3581246/1‑57 (MQ=255) aGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACGAGCGTCGGGTAGGTc > 1:1349213/1‑71 (MQ=255) aGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGt > 1:2815298/1‑70 (MQ=255) gACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCg > 1:56727/1‑71 (MQ=255) aaTTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAg < 1:4246883/71‑1 (MQ=255) aaTTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAg < 1:4545471/71‑1 (MQ=255) tttACGGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa > 1:5341702/1‑71 (MQ=255) tttACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGaa > 1:5669948/1‑71 (MQ=255) tACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTc > 1:6352079/1‑62 (MQ=255) tACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAAt < 1:1873488/70‑1 (MQ=255) tACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAAt > 1:1080085/1‑70 (MQ=255) ccGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGaa < 1:4768029/71‑1 (MQ=255) ccGATACGGGTAGTACCCGTGTCACCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGaa < 1:1528643/71‑1 (MQ=255) aTACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGaa > 1:5245500/1‑68 (MQ=255) aTACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcag < 1:5177951/71‑1 (MQ=255) cGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAg > 1:3332430/1‑51 (MQ=255) cGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagac > 1:2500637/1‑70 (MQ=255) cGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagac < 1:6186002/70‑1 (MQ=255) cGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagac > 1:5078669/1‑70 (MQ=255) cGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGCTCGGCAGAATGAAcagac > 1:4824233/1‑70 (MQ=255) gtagtaCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAAt > 1:6669359/1‑59 (MQ=255) gtagtaCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagaccaga < 1:6607896/71‑1 (MQ=255) gtagtaCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagaccaga < 1:5271932/71‑1 (MQ=255) gtagtaCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagaccaga < 1:4207256/71‑1 (MQ=255) tagtaCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagaccagac < 1:5778975/71‑1 (MQ=255) tagtaCCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAAcagaccagac < 1:3167443/71‑1 (MQ=255) | GGCCAGGGCAACACCCAGAGTACCCGGGATGAAGAACGGATGGTTGAAGACGAATTTACCGATACGGGTAGTACCCGTGTCATCCATCTGTACCGCAGCAACCAGCGTCGGGTAGGTCGGCAGAATGAACAGACCAGAC > minE/2827173‑2827311 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |