| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,937,896 | C→T | 5.1% | intergenic (+23/‑246) | leuX → / → yjhV | tRNA‑Leu/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,937,896 | 0 | C | T | 5.1% | 403.8 / 6.2 | 117 | intergenic (+23/‑246) | leuX/yjhV | tRNA‑Leu/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (54/57); new base T (3/3); total (57/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 125 total aligned reads displayed.
ATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAG > minE/2937827‑2937963 | aTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACt < 1:6168000/71‑1 (MQ=255)aTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACt < 1:1378763/71‑1 (MQ=255)aTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACt < 1:4772459/71‑1 (MQ=255)aTCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACt < 1:5495668/71‑1 (MQ=255) cAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACt > 1:2372065/1‑69 (MQ=255) cAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACt > 1:3948055/1‑69 (MQ=255) cAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACt > 1:5643891/1‑69 (MQ=255) aaCCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAg > 1:2794462/1‑71 (MQ=255) ccGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAg > 1:5392792/1‑69 (MQ=255) ccGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAg > 1:2178859/1‑69 (MQ=255) ccGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAg > 1:5238180/1‑69 (MQ=255) tAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtt < 1:4205942/71‑1 (MQ=255) aGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttt > 1:1806492/1‑71 (MQ=255) aaaTACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAAc > 1:6334280/1‑59 (MQ=255) aaaTACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAAc > 1:1102244/1‑59 (MQ=255) aaaTACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAAc > 1:4205775/1‑59 (MQ=255) aaTACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttttt < 1:4979429/71‑1 (MQ=255) aCGTGCGGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCt < 1:2583296/63‑1 (MQ=38) cGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATg < 1:393371/71‑1 (MQ=255) gTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtat > 1:6165759/1‑61 (MQ=38) gTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtat > 1:5274012/1‑61 (MQ=38) gTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCtat > 1:5485656/1‑61 (MQ=38) ccGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttttt < 1:6767885/63‑1 (MQ=255) ccGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttttt < 1:1549910/63‑1 (MQ=255) cGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGa < 1:4603700/71‑1 (MQ=255) cGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGa < 1:4984249/71‑1 (MQ=255) ttCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttttt < 1:2446743/59‑1 (MQ=255) ttCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttttt < 1:6946146/59‑1 (MQ=255) ttCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAAt > 1:806840/1‑71 (MQ=255) ttCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAAt < 1:4402726/71‑1 (MQ=255) aGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATg > 1:6920570/1‑59 (MQ=255) gTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCa > 1:6957276/1‑69 (MQ=255) gTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgt > 1:1452784/1‑71 (MQ=255) gTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAg > 1:4987281/1‑70 (MQ=255) tCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttt < 1:2045632/52‑1 (MQ=255) tCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttt < 1:4740635/52‑1 (MQ=255) tCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCttttt < 1:5769529/52‑1 (MQ=255) cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttttt < 1:4747817/51‑1 (MQ=255) cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCtttttt < 1:678103/51‑1 (MQ=255) cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgtgt < 1:3286864/70‑1 (MQ=255) cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAgtgt < 1:1071565/70‑1 (MQ=255) cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGtt > 1:6394240/1‑71 (MQ=255) cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGtt > 1:2127126/1‑71 (MQ=255) cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGtt > 1:1995039/1‑71 (MQ=255) cGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGtt > 1:2908750/1‑71 (MQ=255) ggCCTTCGGCACCAAAAGTATGTAAATAGACCTCAGTTGAGGTCt < 1:3979672/45‑1 (MQ=37) ggCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGttt < 1:581579/71‑1 (MQ=255) gCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTATTATGCCTGAAATcc > 1:1069669/1‑63 (MQ=255) cTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTa > 1:4260678/1‑49 (MQ=255) cTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTa > 1:1714028/1‑49 (MQ=255) cGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatcta < 1:1167276/70‑1 (MQ=255) ggCACCAAATCTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatctatc < 1:2635542/71‑1 (MQ=255) ggCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatctatc < 1:4732020/71‑1 (MQ=255) ccAAAAGTATGTAAATAGACCTCAACTGAGGTCttt < 1:4017146/36‑1 (MQ=255) ccAAAAGTATGTAAATAGACCTCAACTGAGGTCttt < 1:4637286/36‑1 (MQ=255) ccAAAAGTATGTAAATAGACCTCAACTGAGGTCttt < 1:3971562/36‑1 (MQ=255) ccAAAAGTATGTAAATAGACCTCAACTGAGGTCttt < 1:859204/36‑1 (MQ=255) ccAAAAGTATGTAAATAGACCTCAACTGAGGTCttt < 1:1021298/36‑1 (MQ=255) ccAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCa < 1:2296528/54‑1 (MQ=255) cAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTta < 1:1668457/60‑1 (MQ=255) cAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTta < 1:3327372/60‑1 (MQ=255) cAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTcc > 1:1588736/1‑71 (MQ=255) cAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTcc > 1:6494212/1‑71 (MQ=255) aaaaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTccc < 1:4591875/71‑1 (MQ=255) aaaaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTccc > 1:756917/1‑71 (MQ=255) aaaaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTcc > 1:1940203/1‑70 (MQ=255) aaaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTccc > 1:524847/1‑70 (MQ=255) aaaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCg > 1:2750919/1‑71 (MQ=255) aaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGc > 1:4507352/1‑71 (MQ=255) aaGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGc < 1:618235/71‑1 (MQ=255) tAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATAtt < 1:5951935/70‑1 (MQ=255) tAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTa < 1:3468809/71‑1 (MQ=255) aaaTAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCttt > 1:5160509/1‑58 (MQ=255) aaaTAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCttt > 1:2294093/1‑58 (MQ=255) aaaTAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCttt > 1:4180147/1‑58 (MQ=255) aaTAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTaa < 1:5707524/70‑1 (MQ=255) tAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATc < 1:6946918/37‑1 (MQ=255) tAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCta < 1:1543165/62‑1 (MQ=255) tAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCta < 1:5010909/62‑1 (MQ=255) tAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAActc > 1:1899351/1‑71 (MQ=255) aGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAActct > 1:5991548/1‑71 (MQ=255) ccTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACtctctc < 1:3201701/71‑1 (MQ=255) cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTaa > 1:2928634/1‑63 (MQ=255) cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTaa > 1:4641804/1‑63 (MQ=255) cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTaa > 1:853461/1‑63 (MQ=255) cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCa < 1:3227817/71‑1 (MQ=255) cTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTATTTATCTATCTTTCCCGCTATATTaa > 1:43984/1‑63 (MQ=255) tCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTa < 1:6527120/61‑1 (MQ=255) tCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTa < 1:3257255/61‑1 (MQ=255) cAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTcc > 1:6125137/1‑50 (MQ=255) cAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTcc > 1:2948341/1‑50 (MQ=255) cAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTcc > 1:5318947/1‑50 (MQ=255) cAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAg > 1:6332965/1‑71 (MQ=255) cAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAg > 1:6608017/1‑71 (MQ=255) cAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAg > 1:3149603/1‑71 (MQ=255) cAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAg > 1:3593915/1‑71 (MQ=255) cAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAg > 1:3596090/1‑71 (MQ=255) atcTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCaa < 1:1447345/67‑1 (MQ=255) cTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTtatctat < 1:5717904/41‑1 (MQ=255) cTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCa < 1:5945787/66‑1 (MQ=255) | ATCAACCGTAGAAATACGTGCCGGTTCGAGTCCGGCCTTCGGCACCAAAAGTATGTAAATAGACCTCAACTGAGGTCTTTTTTTATGCCTGAAATCCAGTGTTTATCTATCTTTCCCGCTATATTAACTCTCTCAAG > minE/2937827‑2937963 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |