| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,833,469 | T→C | 9.6% | T261T (ACT→ACC) | groL → | Cpn60 chaperonin GroEL, large subunit of GroESL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,833,469 | 0 | T | C | 9.6% | 146.1 / 7.2 | 52 | T261T (ACT→ACC) | groL | Cpn60 chaperonin GroEL, large subunit of GroESL |
| Reads supporting (aligned to +/- strand): ref base T (25/22); new base C (2/3); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01 | |||||||||||
GCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGT > minE/2833407‑2833538 | gCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGgttg < 1:1222525/70‑1 (MQ=255) ccAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGgttgt < 1:1453087/70‑1 (MQ=255) aaGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTaa > 1:323911/1‑70 (MQ=255) aaGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTaa > 1:2281238/1‑70 (MQ=255) aGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAaca > 1:284449/1‑71 (MQ=255) aGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAaca > 1:2138933/1‑71 (MQ=255) gCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCAt > 1:845421/1‑70 (MQ=255) aCCGCTGCTGATCATCGCTGAAGATGTAGAAGGCTAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTg < 1:1103263/71‑1 (MQ=255) aCCGCTGCTGATCATCGCTGAAGATGTAGAAGGCTAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTg < 1:1106701/71‑1 (MQ=255) gctgATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCg > 1:1291864/1‑71 (MQ=255) ctgATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAac < 1:557932/54‑1 (MQ=255) gATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTaa > 1:106234/1‑51 (MQ=255) gATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTaa > 1:637397/1‑51 (MQ=255) catcGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGa > 1:2040291/1‑68 (MQ=255) gCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGc > 1:608405/1‑71 (MQ=255) gAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGt < 1:139691/59‑1 (MQ=255) aaGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTaa > 1:2734223/1‑40 (MQ=255) aGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCgg > 1:1806915/1‑71 (MQ=255) gATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGt < 1:2311175/71‑1 (MQ=255) aTGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCAt < 1:1715907/43‑1 (MQ=255) aTGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCAt < 1:2878379/43‑1 (MQ=255) aTGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCAt < 1:810851/43‑1 (MQ=255) tGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTa < 1:187616/71‑1 (MQ=255) gTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTg > 1:411087/1‑54 (MQ=255) gTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTg > 1:2997129/1‑54 (MQ=255) tAGAAGGCGAAGCGCTGTCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTaa < 1:1683449/70‑1 (MQ=255) tAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTaa < 1:593785/70‑1 (MQ=255) tAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTaa < 1:345681/70‑1 (MQ=255) tAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTaa < 1:1725063/70‑1 (MQ=255) aGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAg > 1:502148/1‑71 (MQ=255) aGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAg > 1:2846411/1‑71 (MQ=255) gAAGGCGAAGCGCTGGCGACCCTGGTCGTCAACAACATCCGTGGCAt > 1:1089322/1‑47 (MQ=19) gAAGGCGAAGCGCTGGCGACCCTGGTCGTCAACAACATCCGTGGCAt > 1:1387551/1‑47 (MQ=19) gAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATg < 1:745170/39‑1 (MQ=255) gAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCAt > 1:1379698/1‑47 (MQ=255) gAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGa > 1:1731578/1‑52 (MQ=255) gAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGa > 1:628008/1‑52 (MQ=255) ggCGAAGCGCTGGCGACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCAcc > 1:2033550/1‑71 (MQ=255) cGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCgg < 1:1491823/71‑1 (MQ=255) aGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCt > 1:1038669/1‑71 (MQ=255) aGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCt > 1:1354474/1‑71 (MQ=255) gcgcTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCtt > 1:1673445/1‑71 (MQ=255) gcgcTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCtt < 1:1935551/71‑1 (MQ=255) cgcTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTg < 1:2124970/41‑1 (MQ=255) cTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGaa > 1:2152445/1‑41 (MQ=255) tGGCCACCCTGGTGGTGAACAACATGCGTGGCATCGTCAAGGTCGCGGCTGTCaa < 1:1465786/55‑1 (MQ=255) tGGCCACCCTGGTGGTGAACAACATGCGTGGCATCGTCAAGGTCGCGGCTGTCaa < 1:796575/55‑1 (MQ=255) tGGCCACCCTGGTGGTGAACAACATGCGTGGCATCGTCAAGGTCGCGGCTGTCaa < 1:142543/55‑1 (MQ=255) gCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCAc < 1:2051839/58‑1 (MQ=255) gCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCg < 1:2293606/70‑1 (MQ=255) gCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGa < 1:1774300/71‑1 (MQ=255) aaCTCTGGTTGTTAACACCATGCGTGGCATCGTGaa > 1:447672/1‑36 (MQ=255) ctctGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCg < 1:1239338/44‑1 (MQ=255) ctctGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGAtcgt > 1:879183/1‑71 (MQ=255) | GCCAAAGCAGGCAAACCGCTGCTGATCATCGCTGAAGATGTAGAAGGCGAAGCGCTGGCAACTCTGGTTGTTAACACCATGCGTGGCATCGTGAAAGTCGCTGCGGTTAAAGCACCGGGCTTCGGCGATCGT > minE/2833407‑2833538 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |