Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,036,505 G→A 100% A632A (GCG→GCA rsxC → fused predicted 4Fe‑4S ferredoxin‑type protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,036,5050GA100.0% 94.6 / NA 36A632A (GCG→GCArsxCfused predicted 4Fe‑4S ferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/9);  total (27/9)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC  >  minE/1036437‑1036571
                                                                    |                                                                  
gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:1467111/1‑71 (MQ=21)
gCGGAACCAGAAGAACAGGTCGATCCGCACAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:300478/1‑71 (MQ=255)
gCGGAACCAGAAGAACAGGTCGATCCGCACAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:296855/1‑71 (MQ=255)
 cGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacg                                                                  >  1:1793323/1‑70 (MQ=25)
  ggAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCa                                                                <  1:2724390/71‑1 (MQ=21)
   gAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacgc                                                                 >  1:2846014/1‑69 (MQ=25)
   gAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCaa                                                               <  1:2296850/71‑1 (MQ=21)
            gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAgcacgc                                                                 <  1:2962188/60‑1 (MQ=9)
            gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg                                                           >  1:1055654/1‑66 (MQ=14)
            gaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg                                                           >  1:2463011/1‑66 (MQ=14)
                             cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc                                      >  1:724456/1‑70 (MQ=14)
                              aaaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcc                                      >  1:1505333/1‑69 (MQ=14)
                                        tCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAACCTGGAACAGCAACAGGCTAATGCCg                                     <  1:2900015/60‑1 (MQ=11)
                                          gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          >  1:2133072/1‑69 (MQ=255)
                                            aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                      >  1:3000764/1‑71 (MQ=255)
                                            aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAgg                      >  1:1144402/1‑71 (MQ=255)
                                                gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa                  <  1:823804/71‑1 (MQ=255)
                                                gCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa                  <  1:2661915/71‑1 (MQ=255)
                                                 cTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                 >  1:179815/1‑71 (MQ=255)
                                                 cTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGAt                 >  1:2153622/1‑71 (MQ=255)
                                                   aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc               >  1:1138976/1‑71 (MQ=255)
                                                    ttGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          >  1:343838/1‑59 (MQ=255)
                                                     tGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg                               <  1:2921944/53‑1 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa                              >  1:2569603/1‑53 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg            >  1:1219709/1‑71 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgag            >  1:325411/1‑69 (MQ=255)
                                                      gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAACGCCGAGCCAGAACAACAGGTCGATCcgcg            >  1:1582045/1‑71 (MQ=14)
                                                        ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCgcgc           >  1:1501657/1‑70 (MQ=255)
                                                         cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc             >  1:1242004/1‑67 (MQ=255)
                                                         cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc             >  1:535065/1‑67 (MQ=255)
                                                              cAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGa                              <  1:2477368/45‑1 (MQ=255)
                                                               aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaacaa                          <  1:180829/48‑1 (MQ=255)
                                                               aaaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgc   <  1:2757856/71‑1 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac                            >  1:1251531/1‑45 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac                            >  1:1908143/1‑45 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac                            >  1:637381/1‑45 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgcggcc  >  1:2475988/1‑71 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:122425/1‑71 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:1963241/1‑71 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:1019973/1‑71 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgcc  >  1:1730545/1‑71 (MQ=255)
                                                                aaGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAgccgc   >  1:2762262/1‑70 (MQ=255)
                                                                    |                                                                  
GCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCC  >  minE/1036437‑1036571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: