| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,356,166 | G→C | 5.0% | L154V (CTA→GTA) | yehA ← | predicted fimbrial‑like adhesin protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,356,166 | 0 | G | C | 5.0% | 262.8 / 3.2 | 79 | L154V (CTA→GTA) | yehA | predicted fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base G (44/31); new base C (2/2); total (46/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
GTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCAT > minE/1356100‑1356235 | gtgCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGaaa < 1:1289140/70‑1 (MQ=255) ccAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTg > 1:20913/1‑70 (MQ=255) ccAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTg < 1:2228833/70‑1 (MQ=255) aaTGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGaaa < 1:2140971/65‑1 (MQ=255) aaTGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTAc < 1:2351861/71‑1 (MQ=255) aTGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGaaa < 1:233423/64‑1 (MQ=255) gCATTTCTGTAGCTATTTACACATGCATCCCATGAGTTGCCACCTAATAAACACAGTAGAAAt > 1:655185/1‑63 (MQ=255) gCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAAt > 1:1958561/1‑63 (MQ=255) cATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGaaa > 1:810472/1‑61 (MQ=255) tttCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGt < 1:2944320/63‑1 (MQ=255) ttCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCa > 1:533468/1‑71 (MQ=255) tCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCa > 1:2384146/1‑70 (MQ=255) tCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCAc > 1:488702/1‑71 (MQ=255) tCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCAc > 1:1491498/1‑71 (MQ=255) tCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCAc > 1:1958222/1‑71 (MQ=255) tCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCAc > 1:1030773/1‑71 (MQ=255) cTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCAc < 1:674544/70‑1 (MQ=255) cTGTAGCTATTTACACATGCAT‑CCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTc > 1:297341/1‑71 (MQ=255) tAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACt < 1:1163974/68‑1 (MQ=255) gCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCcgc > 1:1067019/1‑71 (MQ=255) cTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCcgcg > 1:2455474/1‑71 (MQ=255) aTTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCgtg > 1:2929546/1‑71 (MQ=255) aTTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCgtg > 1:1116607/1‑71 (MQ=255) tttACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAAt < 1:2187096/48‑1 (MQ=255) tttACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCgt < 1:1846071/69‑1 (MQ=255) tACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTc > 1:2587130/1‑70 (MQ=255) acacATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCg > 1:2259948/1‑54 (MQ=255) acacATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCg > 1:934749/1‑54 (MQ=255) acacATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCg > 1:756941/1‑54 (MQ=255) acacATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCg > 1:760459/1‑54 (MQ=255) acacATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCg < 1:1313823/70‑1 (MQ=255) acacATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCg < 1:846364/70‑1 (MQ=255) acacATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCg < 1:1256894/70‑1 (MQ=255) cacaTGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCgt < 1:1134271/65‑1 (MQ=255) acaTGCATCCCATGACTTGCCACCTAATAAACGCAGTACaatt < 1:468601/43‑3 (MQ=38) acaTGCATCCCATGACTTGCCACCTAATAAACGCAGTACaatt < 1:123414/43‑3 (MQ=38) acaTGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCgatg < 1:1967017/71‑1 (MQ=255) gtGCATCCCATGACTTGCCACCTAATAAACACAGTACAAtttc > 1:2869799/2‑42 (MQ=255) aTGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCgatgat > 1:1050815/1‑71 (MQ=255) aTGCATCCCATGACTTGCCACCTAATAAACACAGTACAAtttc > 1:1508034/1‑42 (MQ=255) tGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGatgatg < 1:3024132/71‑1 (MQ=255) tGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGatgatg < 1:2935425/71‑1 (MQ=255) tGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGatgatg < 1:1171239/71‑1 (MQ=255) cATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGatgatg > 1:2148252/1‑69 (MQ=255) aTCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTg < 1:842924/44‑1 (MQ=255) cccATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGatgatg < 1:2878202/66‑1 (MQ=255) ccATGACTTGCCACCTAATAAACACAGTAGAAATTGTa > 1:658419/1‑38 (MQ=255) ccATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCgg < 1:1854079/44‑1 (MQ=255) ccATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCAc > 1:590283/1‑71 (MQ=255) cATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCgatg < 1:2875805/61‑1 (MQ=255) tGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGaa > 1:1551197/1‑64 (MQ=255) aCTTGCCACCTAATAAACACATTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGAt < 1:1238988/71‑1 (MQ=255) aCTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCgtgt > 1:2171089/1‑52 (MQ=255) aCTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCga > 1:2600324/1‑55 (MQ=255) aCTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCga > 1:1080423/1‑55 (MQ=255) aCTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCga > 1:2467030/1‑55 (MQ=255) aCTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTg > 1:2488579/1‑68 (MQ=255) aCTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGa > 1:857630/1‑70 (MQ=255) ttGCCACCTAATAAACACAGTAGAAATTGTACTGc < 1:291521/35‑1 (MQ=255) ccACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGt < 1:2399214/71‑1 (MQ=255) cACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGAt < 1:382908/65‑1 (MQ=255) ccTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCg > 1:2174184/1‑70 (MQ=255) ccTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCg > 1:3032580/1‑70 (MQ=255) ccTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCg > 1:1879206/1‑70 (MQ=255) ccTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCg > 1:468241/1‑70 (MQ=255) cTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCa < 1:904004/71‑1 (MQ=255) aataaACACAGTAGAAATTGTACTGCGGCACTCCCGCgtgt > 1:3051317/1‑41 (MQ=255) taaACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAacac < 1:264785/71‑1 (MQ=255) aaaCACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCgatga > 1:2653440/1‑44 (MQ=255) aaCACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCa > 1:1442937/1‑71 (MQ=255) acacAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGaa > 1:1512177/1‑46 (MQ=255) aGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCa > 1:810197/1‑45 (MQ=255) gTAGAAATTGTACTGCGGCACTCCCGCGTGTCgatgat < 1:2220567/38‑1 (MQ=255) gTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAAt > 1:1053423/1‑53 (MQ=255) tAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATaa < 1:2706929/51‑1 (MQ=255) tAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATaa < 1:1468108/51‑1 (MQ=255) tAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAatca > 1:2376121/1‑71 (MQ=255) tAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAatca > 1:2853540/1‑71 (MQ=255) aGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAatcat > 1:1174606/1‑71 (MQ=255) | GTGCCAATGCATTTCTGTAGCTATTTACACATGCATCCCATGACTTGCCACCTAATAAACACAGTAGAAATTGTACTGCGGCACTCCCGCGTGTCGATGATGAAGCACTGGATAATGTCGCAACACCATTAATCAT > minE/1356100‑1356235 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |