| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 954,077 | T→C | 6.4% | M506V (ATG→GTG) | gadC ← | predicted glutamate:gamma‑aminobutyric acid antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 954,077 | 0 | T | C | 6.4% | 457.1 / 14.8 | 144 | M506V (ATG→GTG) | gadC | predicted glutamate:gamma‑aminobutyric acid antiporter |
| Reads supporting (aligned to +/- strand): ref base T (78/56); new base C (2/7); total (81/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.34e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
Only 100 of 178 total aligned reads displayed.
AAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTG > minE/954007‑954147 | cGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAata < 1:62992/71‑1 (MQ=255) aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGATATGGTTAGTGTTTCTTGTCATTCATCACAata > 1:1952576/1‑69 (MQ=255) aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAata > 1:1870679/1‑69 (MQ=255) aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAata > 1:2893125/1‑69 (MQ=255) tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac < 1:1301446/64‑1 (MQ=255) tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAg < 1:2169840/71‑1 (MQ=255) tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAg > 1:491735/1‑71 (MQ=255) aaTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa > 1:3445895/1‑64 (MQ=255) aaTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa > 1:2983884/1‑64 (MQ=255) aaTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACtatatt < 1:3376227/70‑3 (MQ=255) aaTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACtatatt < 1:1129178/70‑3 (MQ=255) aaTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACtatatt < 1:3154243/70‑3 (MQ=255) aTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg > 1:85379/1‑70 (MQ=255) aTCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt > 1:1158985/1‑71 (MQ=255) gCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGaa > 1:244351/1‑71 (MQ=255) gCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGa < 1:3262181/70‑1 (MQ=255) cTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAAc < 1:1912590/71‑1 (MQ=255) cTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAAc < 1:1558021/71‑1 (MQ=255) cTCCCTTGTCTCATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAAc < 1:530336/71‑1 (MQ=255) tCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAAc < 1:1770007/70‑1 (MQ=255) cccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt > 1:3441914/1‑71 (MQ=255) cccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt > 1:2383579/1‑71 (MQ=255) ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac < 1:2256438/51‑1 (MQ=255) ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGt < 1:751160/70‑1 (MQ=255) cTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGc < 1:1641975/71‑1 (MQ=255) cTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGc < 1:1268171/71‑1 (MQ=255) ttGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac < 1:2936079/49‑1 (MQ=255) ttGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATcac > 1:2826841/1‑49 (MQ=255) tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgt > 1:1290871/1‑56 (MQ=255) tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtggt > 1:960302/1‑61 (MQ=255) gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAAtata > 1:2194466/1‑53 (MQ=255) gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg > 1:1724097/1‑71 (MQ=255) gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg > 1:781068/1‑71 (MQ=255) gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg > 1:1412473/1‑71 (MQ=255) gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcg > 1:1024719/1‑71 (MQ=255) tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTgtgg > 1:942989/1‑58 (MQ=255) tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgc < 1:2952167/71‑1 (MQ=255) tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgc < 1:703317/71‑1 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGtcattcat < 1:3230433/39‑1 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa > 1:400993/1‑44 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACaa > 1:1140836/1‑44 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgtg > 1:2132425/1‑51 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAgt > 1:692033/1‑50 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgcg < 1:2129264/68‑1 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCAcgcg < 1:1902106/68‑1 (MQ=255) ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGt > 1:1056221/1‑71 (MQ=255) aaCCATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACTATATtgtgat < 1:1343889/53‑3 (MQ=25) ccATTCAGACATGGTTAGTGTTTCTTGTCATTCACCACACTATATTgtgatg > 1:1217438/1‑52 (MQ=25) aTTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAgg > 1:2896765/1‑70 (MQ=255) aTTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAgg > 1:2815715/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGc > 1:2296462/1‑54 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag < 1:2981317/70‑1 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:996416/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:458371/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:874199/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag < 1:2846454/70‑1 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:1191265/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag < 1:1613435/70‑1 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:1651339/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag > 1:2149350/1‑70 (MQ=255) cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGgaag < 1:1927198/70‑1 (MQ=255) gACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag < 1:3035729/71‑1 (MQ=255) cATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag > 1:1805635/1‑69 (MQ=255) aaTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag < 1:638681/68‑1 (MQ=255) aaTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGaagaag < 1:18100/68‑1 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCa > 1:1034516/1‑50 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCa > 1:2021853/1‑50 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGa > 1:2691548/1‑71 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGa > 1:2051987/1‑71 (MQ=255) aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGa > 1:3141989/1‑71 (MQ=255) ttAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtt < 1:3166868/71‑1 (MQ=255) aGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCttt < 1:1388746/70‑1 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg > 1:3274778/1‑71 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg < 1:157635/71‑1 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg > 1:2456537/1‑71 (MQ=255) gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg < 1:1686052/71‑1 (MQ=255) tgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTgg < 1:1981168/71‑1 (MQ=255) tgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTg < 1:1374523/70‑1 (MQ=255) tttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTgg > 1:308453/1‑69 (MQ=255) tttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTgg > 1:2547499/1‑69 (MQ=255) tGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTgg > 1:508715/1‑64 (MQ=255) tcattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCt < 1:1073765/70‑1 (MQ=255) tcattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCt < 1:220718/70‑1 (MQ=255) tcattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTg < 1:3238450/71‑1 (MQ=255) attcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt > 1:776880/1‑58 (MQ=255) attcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt > 1:734143/1‑58 (MQ=255) attcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt > 1:2966210/1‑58 (MQ=255) attcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt > 1:2068325/1‑58 (MQ=255) attcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCtttt > 1:3205395/1‑58 (MQ=255) attcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCg < 1:1854005/64‑1 (MQ=255) attcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCg < 1:1485001/64‑1 (MQ=255) ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACt > 1:2233249/1‑71 (MQ=255) tcatcaCAATTTAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTg > 1:1638606/1‑71 (MQ=255) tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGtt > 1:2479346/1‑64 (MQ=255) tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGtt > 1:908013/1‑64 (MQ=255) tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTg > 1:2029529/1‑71 (MQ=255) tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTg > 1:2767694/1‑71 (MQ=255) catcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGt > 1:3459033/1‑71 (MQ=255) tcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTg < 1:1439616/71‑1 (MQ=255) tcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTg < 1:579799/71‑1 (MQ=255) | AAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTG > minE/954007‑954147 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |