| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,692,655 | A→C | 10.3% | S107S (TCT→TCG) | iscU ← | scaffold protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,692,655 | 0 | A | C | 10.3% | 146.2 / 13.6 | 58 | S107S (TCT→TCG) | iscU | scaffold protein |
| Reads supporting (aligned to +/- strand): ref base A (20/32); new base C (2/4); total (22/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
ATTTTGCTTCACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGC > minE/1692591‑1692723 | aTTTTGCTTCACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAg > 1:3029861/1‑71 (MQ=255)aTTTTGCTTCACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAg > 1:24903/1‑71 (MQ=255) gCTTCACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGaa < 1:1554593/70‑1 (MQ=255) cTTCACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACa < 1:523401/64‑1 (MQ=255) cTTCACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACa < 1:2412500/64‑1 (MQ=255) ttCACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAAttt > 1:199710/1‑71 (MQ=255) cACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAAttt < 1:955476/69‑1 (MQ=255) cACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAAttt < 1:6758/69‑1 (MQ=255) cACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAAttt < 1:2710043/69‑1 (MQ=255) aCGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCa > 1:1347495/1‑71 (MQ=255) tttGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACcg > 1:1702770/1‑71 (MQ=255) tttGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACcg < 1:2410453/71‑1 (MQ=255) tGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACcggc > 1:3355487/1‑71 (MQ=255) aGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTg < 1:2637660/49‑1 (MQ=255) tCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGaa > 1:716733/1‑42 (MQ=255) tCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGaa > 1:1823433/1‑42 (MQ=255) tCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGaa > 1:1309593/1‑42 (MQ=255) tCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGaa > 1:204809/1‑42 (MQ=255) cGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGtt > 1:1497342/1‑71 (MQ=255) cGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGtt > 1:3424777/1‑71 (MQ=255) cGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGt > 1:2907000/1‑70 (MQ=255) aaTGGCGGCTTTGATCGCGTCTTCTGCCAGATTAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGttctt < 1:3380813/71‑1 (MQ=255) aaTGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGttctt > 1:1908823/1‑71 (MQ=255) aTGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAAtttt > 1:1544570/1‑47 (MQ=255) aTGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCa < 1:276956/63‑1 (MQ=255) tGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGaa < 1:2225560/35‑1 (MQ=255) tGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGaa < 1:1981750/35‑1 (MQ=255) ggcggcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAg > 1:3100382/1‑71 (MQ=255) ggcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCaa < 1:2096005/71‑1 (MQ=255) gcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAAttt < 1:1166503/40‑1 (MQ=255) gcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTGAAGTTCTTCAGCaa < 1:2495944/70‑1 (MQ=255) gcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCaa < 1:1854766/70‑1 (MQ=255) gcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAat < 1:2015791/71‑1 (MQ=255) gcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAat < 1:2523917/71‑1 (MQ=255) cTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAat < 1:3324123/70‑1 (MQ=255) ttGATCGCGTCTTCGGCCAGGATCGAGCAGTGAATCTTGACcggc < 1:1181764/45‑1 (MQ=255) ttGATCGCGTCTTCGGCCAGGATCGAGCAGTGAATCTTGACcggc < 1:2998770/45‑1 (MQ=255) ttGATCGCGTCTTCGGCCAGGATCGAGCAGTGAATCTTGACcggc < 1:1043055/45‑1 (MQ=255) ttGATCGCGTCTTCGGCCAGGATCGAGCAGTGAATCTTGACcggc < 1:1873922/45‑1 (MQ=255) gATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAata < 1:1494418/67‑1 (MQ=255) gATCGCGTCTTCGGCCAGGATCGAGCAGTGAATCTTGACcggcgg > 1:1606743/1‑45 (MQ=255) gcgTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGtt < 1:846428/71‑1 (MQ=255) cgTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGttt < 1:3058147/71‑1 (MQ=255) ttctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGc < 1:366366/54‑1 (MQ=255) ttctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTg < 1:2511449/70‑1 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAg > 1:2668090/1‑43 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAg < 1:836346/51‑1 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCaa > 1:3256441/1‑54 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCaa > 1:1956211/1‑54 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTg < 1:1436081/68‑1 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTg < 1:2089608/68‑1 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGa < 1:1455435/69‑1 (MQ=255) cggCCAGGATCGAGCAGTGAATCTTGACCGGCGGCAGGGCCAGttc > 1:2866152/3‑46 (MQ=255) ccAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATc < 1:2870129/68‑1 (MQ=255) gAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATc < 1:1315728/65‑1 (MQ=255) gAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATc < 1:1075108/65‑1 (MQ=255) gAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATc < 1:2593588/65‑1 (MQ=255) aTAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTgcgc > 1:1221539/1‑71 (MQ=255) | ATTTTGCTTCACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGC > minE/1692591‑1692723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |