| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,692,670 | C→G | 12.5% | V102V (GTG→GTC) | iscU ← | scaffold protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,692,670 | 0 | C | G | 12.5% | 131.1 / 10.9 | 48 | V102V (GTG→GTC) | iscU | scaffold protein |
| Reads supporting (aligned to +/- strand): ref base C (15/27); new base G (2/4); total (17/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.83e-01 | |||||||||||
ACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTC > minE/1692601‑1692739 | aCGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCa > 1:1347495/1‑71 (MQ=255) tttGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACcg < 1:2410453/71‑1 (MQ=255) tttGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACcg > 1:1702770/1‑71 (MQ=255) tGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACcggc > 1:3355487/1‑71 (MQ=255) cGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGtt > 1:1497342/1‑71 (MQ=255) cGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGtt > 1:3424777/1‑71 (MQ=255) cGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGt > 1:2907000/1‑70 (MQ=255) aaTGGCGGCTTTGATCGCGTCTTCTGCCAGATTAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGttctt < 1:3380813/71‑1 (MQ=255) aaTGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGttctt > 1:1908823/1‑71 (MQ=255) aTGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCa < 1:276956/63‑1 (MQ=255) ggcggcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAg > 1:3100382/1‑71 (MQ=255) ggcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCaa < 1:2096005/71‑1 (MQ=255) gcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTGAAGTTCTTCAGCaa < 1:2495944/70‑1 (MQ=255) gcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCaa < 1:1854766/70‑1 (MQ=255) gcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAat < 1:2015791/71‑1 (MQ=255) gcTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAat < 1:2523917/71‑1 (MQ=255) cTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAat < 1:3324123/70‑1 (MQ=255) ttGATCGCGTCTTCGGCCAGGATCGAGCAGTGAATCTTGACcggc < 1:1043055/45‑1 (MQ=255) ttGATCGCGTCTTCGGCCAGGATCGAGCAGTGAATCTTGACcggc < 1:1181764/45‑1 (MQ=255) ttGATCGCGTCTTCGGCCAGGATCGAGCAGTGAATCTTGACcggc < 1:1873922/45‑1 (MQ=255) ttGATCGCGTCTTCGGCCAGGATCGAGCAGTGAATCTTGACcggc < 1:2998770/45‑1 (MQ=255) gATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAata < 1:1494418/67‑1 (MQ=255) gATCGCGTCTTCGGCCAGGATCGAGCAGTGAATCTTGACcggcgg > 1:1606743/1‑45 (MQ=255) gcgTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGtt < 1:846428/71‑1 (MQ=255) cgTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGttt < 1:3058147/71‑1 (MQ=255) ttctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGc < 1:366366/54‑1 (MQ=255) ttctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTg < 1:2511449/70‑1 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAg > 1:2668090/1‑43 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAg < 1:836346/51‑1 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCaa > 1:1956211/1‑54 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCaa > 1:3256441/1‑54 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTg < 1:2089608/68‑1 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTg < 1:1436081/68‑1 (MQ=255) ctGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGa < 1:1455435/69‑1 (MQ=255) cggCCAGGATCGAGCAGTGAATCTTGACCGGCGGCAGGGCCAGttc > 1:2866152/3‑46 (MQ=255) ccAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATc < 1:2870129/68‑1 (MQ=255) gAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATc < 1:1315728/65‑1 (MQ=255) gAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATc < 1:2593588/65‑1 (MQ=255) gAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATc < 1:1075108/65‑1 (MQ=255) aTAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTgcgc > 1:1221539/1‑71 (MQ=255) aaCAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTgc < 1:1600955/65‑1 (MQ=255) cAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTtcgtc < 1:3302325/71‑1 (MQ=255) cAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTtcgtc > 1:1116636/1‑71 (MQ=255) gTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTtcgtc > 1:2885693/1‑69 (MQ=255) aaTTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGcc > 1:280225/1‑55 (MQ=255) aaTTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCgaga < 1:2815253/70‑1 (MQ=255) aTTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAat < 1:1034173/35‑1 (MQ=255) aTTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCgagag < 1:2215765/70‑1 (MQ=255) cACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTc < 1:1095744/70‑1 (MQ=255) cACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTc < 1:2423787/70‑1 (MQ=255) cACCGGCGGCAGTTCAAGTTCTTCAGCAATATAGGTGTTTTTg > 1:2470378/1‑43 (MQ=255) | ACGTTTGCTTTTATAGTCCGCAATGGCGGCTTTGATCGCGTCTTCTGCCAGAATAGAACAGTGAATTTTCACCGGCGGCAGTTCAAGTTCTTCAGCAATATCGGTGTTTTTGATCGCCTGCGCTTCGTCGAGAGACTTC > minE/1692601‑1692739 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |