| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,816,284 | T→C | 10.0% | K217K (AAA→AAG) | recA ← | DNA strand exchange and recombination protein with protease and nuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,816,284 | 0 | T | C | 10.0% | 175.8 / 9.5 | 60 | K217K (AAA→AAG) | recA | DNA strand exchange and recombination protein with protease and nuclease activity |
| Reads supporting (aligned to +/- strand): ref base T (26/28); new base C (3/3); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.34e-01 | |||||||||||
ACCCACCACGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATA > minE/1816215‑1816344 | aCCCACCACGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAAtt > 1:3090448/1‑71 (MQ=255) cccACCACGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAAttt < 1:2371690/71‑1 (MQ=255) caccacGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAAttt > 1:2306463/1‑69 (MQ=255) caccacGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAAttt > 1:397485/1‑69 (MQ=255) caccacGTTTTCACCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAAttt > 1:1908758/1‑69 (MQ=255) caccacGTTTTCACCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAAtt > 1:1912736/1‑68 (MQ=255) accacGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAg > 1:1110647/1‑71 (MQ=255) ccacGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTc < 1:282270/68‑1 (MQ=255) cacGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAgcg < 1:3342749/71‑1 (MQ=255) cacGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAgcg < 1:2982892/71‑1 (MQ=255) acGTTTTCGCCCTCTTTCACCGCGCGGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAgcgc > 1:1667655/1‑71 (MQ=255) cGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGcgcg < 1:1747289/71‑1 (MQ=255) cGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGcgcg < 1:1942852/71‑1 (MQ=255) cGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGcgcg < 1:2930665/71‑1 (MQ=255) cGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGcgcg < 1:1524326/71‑1 (MQ=255) gTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTc > 1:1281405/1‑64 (MQ=255) gTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTc > 1:1511858/1‑64 (MQ=255) gTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGcgcg > 1:2463947/1‑70 (MQ=255) tttCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTa > 1:1539961/1‑71 (MQ=255) ttCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTac > 1:1655465/1‑71 (MQ=255) gCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTaccac < 1:647448/71‑1 (MQ=255) cccTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTaccacc > 1:1768035/1‑71 (MQ=255) cccTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTaccacc > 1:1859678/1‑71 (MQ=255) cccTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTaccacc > 1:571632/1‑71 (MQ=255) ttCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGt > 1:1852267/1‑71 (MQ=255) cACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCa > 1:2861118/1‑50 (MQ=255) cACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTgg > 1:2309427/1‑71 (MQ=255) aCCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGt < 1:2613020/71‑1 (MQ=255) cgcgCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTac > 1:598213/1‑56 (MQ=255) gcgcCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAAttt > 1:2835945/1‑44 (MQ=255) gcgcCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAAttt > 1:801481/1‑44 (MQ=255) gcgcCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAAttt > 1:944781/1‑44 (MQ=255) ccGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCgg < 1:3023153/71‑1 (MQ=255) ccGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCgg < 1:1646926/71‑1 (MQ=255) gacgGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCg < 1:3101420/71‑1 (MQ=255) gacgGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCg < 1:749540/71‑1 (MQ=255) gacgGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCg < 1:695987/71‑1 (MQ=255) gacgGATGTCCATGCGCACGGAGGCGTAGAACTTCAGTGCGtt < 1:485267/43‑1 (MQ=255) gacgGATGTCCATGCGCACGGAGGCGTAGAACTTCAGTGCGtt < 1:1210086/43‑1 (MQ=255) gacgGATGTCCATGCGCACGGAGGCGTAGAACTTCAGTGCGtt < 1:1557634/43‑1 (MQ=255) acgGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCgg < 1:116627/50‑1 (MQ=255) acgGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCgg < 1:692674/50‑1 (MQ=255) acgGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCgg < 1:3015873/50‑1 (MQ=255) acgGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCgg < 1:198053/50‑1 (MQ=255) cgGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGaa > 1:151706/1‑71 (MQ=255) cgGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGaa > 1:2463824/1‑71 (MQ=255) ggATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCgg < 1:407249/48‑1 (MQ=255) ggATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACAACCGGTa > 1:2031346/1‑50 (MQ=255) gTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATca > 1:1114898/1‑71 (MQ=255) gTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATca > 1:2874121/1‑71 (MQ=255) gaCGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACAcc > 1:2948023/1‑70 (MQ=255) gaCGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACAcc > 1:1993333/1‑70 (MQ=255) aaCAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAAttt < 1:149718/71‑1 (MQ=255) aaCAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAAttt < 1:71285/71‑1 (MQ=255) aCAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAAtttt < 1:369028/71‑1 (MQ=255) aCAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAAtttt < 1:3294925/71‑1 (MQ=255) agagGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCa < 1:1879185/71‑1 (MQ=255) gagGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCa > 1:1430072/1‑70 (MQ=255) gagGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCa > 1:1184975/1‑70 (MQ=255) gagGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCa > 1:2545682/1‑70 (MQ=255) gagGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCa > 1:104092/1‑70 (MQ=255) agGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATa < 1:1644776/71‑1 (MQ=255) agGCGTAGAACTTCAGTGCGTTACCGCCGGTGGTGGTTTCCGGGTTGCCGAACATGACGCCGATCTTCAtg > 1:510541/1‑70 (MQ=255) agGCGTAGAACTTCAGTGCGTTACCGCCGGTGGTGGTTTCCGGGTTGCCGAACATGACGCCGATCTTCAtg > 1:2716624/1‑70 (MQ=255) agGCGTAGAACTTCAGTGCGTTACCGCCGGTGGTGGTTTCCGGGTTGCCGAACATGACGCCGATCTTCAtg > 1:1114137/1‑70 (MQ=255) aaTTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTAcc > 1:2488896/1‑41 (MQ=255) aaTTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACAt < 1:1817197/47‑1 (MQ=255) aaTTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACAt < 1:640349/47‑1 (MQ=255) aaTTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACAt < 1:2765711/47‑1 (MQ=255) | ACCCACCACGTTTTCGCCCTCTTTCACCGCGCCGATACGACGGATGTCGAGACGAACAGAGGCGTAGAATTTCAGCGCGTTACCACCGGTAGTGGTTTCCGGGTTACCGAACATCACACCAATTTTCATA > minE/1816215‑1816344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |