| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,910,405 | C→T | 6.4% | V117M (GTG→ATG) | ppdB ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,910,405 | 0 | C | T | 6.4% | 303.5 / 8.2 | 94 | V117M (GTG→ATG) | ppdB | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (52/35); new base T (3/3); total (55/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.84e-01 | |||||||||||
ATCCGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGT > minE/1910337‑1910470 | aTCCGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACAt > 1:671063/1‑71 (MQ=255)aTCCGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACAt > 1:476226/1‑71 (MQ=255)aTCCGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACAt < 1:324445/71‑1 (MQ=255)aTCCGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACAt > 1:1829563/1‑71 (MQ=255) cGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGc < 1:1063562/70‑1 (MQ=255) cGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGc > 1:3410093/1‑70 (MQ=255) cGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCt > 1:485404/1‑71 (MQ=255) cGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCt > 1:974548/1‑71 (MQ=255) cGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCt > 1:3207845/1‑71 (MQ=255) cGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCt > 1:3178319/1‑71 (MQ=255) cGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCt > 1:3095044/1‑71 (MQ=255) cGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCt > 1:2183761/1‑71 (MQ=255) ggATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGc < 1:2828128/69‑1 (MQ=255) aTTAGTGAGTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCt > 1:3399860/1‑71 (MQ=255) aTTAGTGACTTTATCCCAGCCCTTACCTTTACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCt < 1:1029452/71‑1 (MQ=255) aTTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCt < 1:346332/71‑1 (MQ=255) aTTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCt > 1:3063867/1‑71 (MQ=255) ttAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGcaca > 1:2445197/1‑63 (MQ=255) ttAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCtt < 1:1717656/71‑1 (MQ=255) ttAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCtt < 1:2877395/71‑1 (MQ=255) tAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCtt > 1:1325013/1‑70 (MQ=255) tAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCtt < 1:3369286/70‑1 (MQ=255) tAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCtt > 1:1107418/1‑70 (MQ=255) tAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCtt > 1:1119564/1‑70 (MQ=255) gTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAg < 1:605827/71‑1 (MQ=255) tGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCa < 1:1041491/69‑1 (MQ=255) gACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCa < 1:3243085/68‑1 (MQ=255) aCTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGAc > 1:3457388/1‑70 (MQ=255) cTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGa < 1:316333/71‑1 (MQ=255) cTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGa < 1:2211531/71‑1 (MQ=255) cTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGa < 1:1120204/71‑1 (MQ=255) cTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGa < 1:1198705/71‑1 (MQ=255) tttATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGa < 1:3107262/70‑1 (MQ=255) tATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGaaa > 1:694072/1‑70 (MQ=255) tATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGaaa > 1:110916/1‑70 (MQ=255) tATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAAt > 1:1180294/1‑71 (MQ=255) tCCCAGCCCTT‑CCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCa < 1:1906998/71‑1 (MQ=255) ccAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCa < 1:2543326/70‑1 (MQ=255) gCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCt > 1:3136228/1‑71 (MQ=255) gCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCt > 1:3285023/1‑71 (MQ=255) aCCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCaa > 1:417861/1‑62 (MQ=255) aCCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCaa > 1:2981047/1‑62 (MQ=255) aCCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCaa > 1:1585026/1‑62 (MQ=255) aCCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCaa > 1:3419276/1‑62 (MQ=255) aCCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTGCAGACGAAATCCaa > 1:316787/1‑62 (MQ=255) aCCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCATTCAGACGAAATCCaa > 1:1738356/1‑62 (MQ=255) cTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGAc < 1:943535/71‑1 (MQ=255) tCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGActc > 1:2816209/1‑71 (MQ=255) acaGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGActct > 1:2420437/1‑70 (MQ=255) acaGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCtt > 1:1145429/1‑71 (MQ=255) aGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCttt > 1:2973923/1‑70 (MQ=255) aGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCttt > 1:1807333/1‑70 (MQ=255) tCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGt < 1:2025017/71‑1 (MQ=255) cACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcg < 1:2323276/71‑1 (MQ=255) cACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcg < 1:898901/71‑1 (MQ=255) cACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcg < 1:1871126/71‑1 (MQ=255) cACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcg < 1:1905807/71‑1 (MQ=255) aCCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATc > 1:2673645/1‑46 (MQ=255) aCCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATc > 1:2558999/1‑46 (MQ=255) aCCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATc > 1:685148/1‑46 (MQ=255) aCCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATc > 1:1927402/1‑46 (MQ=255) ccGCGTAGCGTTTCCAGCATATGCTCCTTCAGACGa < 1:1242799/36‑1 (MQ=255) ccGCGTAGCGTTTCCAGCATATGCTCCTTCAGACGa < 1:1185896/36‑1 (MQ=255) ccGCGTAGCGTTTCCAGCATATGCTCCTTCAGACGa < 1:837445/36‑1 (MQ=255) ccGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcgcg < 1:1590980/71‑1 (MQ=255) ccGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcgcg < 1:1586846/71‑1 (MQ=255) ccGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcgc > 1:1455604/1‑70 (MQ=255) gcgTAGCGTTTCCAGCATATGCTCCTTCAGACGaaa > 1:1948415/1‑36 (MQ=255) gcgTAGCGTTTCCAGCATATGCTCCTTCAGACGaaa > 1:676077/1‑36 (MQ=255) gcgTAGCGTTTCCAGCATATGCTCCTTCAGACGaaa > 1:745774/1‑36 (MQ=255) gcgTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGa > 1:3130481/1‑70 (MQ=255) gcgTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGa > 1:2701499/1‑70 (MQ=255) tAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCa < 1:636790/37‑1 (MQ=255) tAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCa < 1:1210415/37‑1 (MQ=255) tAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATc < 1:2330495/40‑1 (MQ=255) tAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATc < 1:715156/40‑1 (MQ=255) tAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATc < 1:2615510/40‑1 (MQ=255) gCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTg > 1:580392/1‑40 (MQ=255) gCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcgc < 1:1976123/63‑1 (MQ=255) gCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGAt > 1:752686/1‑66 (MQ=255) gCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGAt > 1:989743/1‑66 (MQ=255) gCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGAt > 1:3201235/1‑66 (MQ=255) gCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGAt > 1:2992895/1‑66 (MQ=255) gCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGAt > 1:152282/1‑66 (MQ=255) cGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCgg < 1:1523648/57‑1 (MQ=255) cGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGa > 1:2655848/1‑64 (MQ=255) cGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGa < 1:3443916/71‑1 (MQ=255) cGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGACGGACTCTTTTACCGGTTCGCGATCCCAGa < 1:3118631/71‑1 (MQ=255) tttCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATcc > 1:731932/1‑65 (MQ=255) tttCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATcc > 1:981010/1‑65 (MQ=255) tttCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATa > 1:2418488/1‑71 (MQ=255) cAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCgg > 1:853434/1‑38 (MQ=255) cAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCgg > 1:1914761/1‑38 (MQ=255) cAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGt > 1:3154711/1‑71 (MQ=255) gCAGATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTcg < 1:2857445/53‑1 (MQ=255) acaTGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCa < 1:2009034/59‑1 (MQ=255) | ATCCGGATTAGTGACTTTATCCCAGCCCTTACCTTCACAGGATGTCGCACCGCGTAGCGTTTCCAGCACATGCTCCTTCAGACGAAATCCAATCTGGTCGGACTCTTTTACCGGTTCGCGATCCCAGATACCGT > minE/1910337‑1910470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |