| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,102,851 | G→A | 8.6% | W143* (TGG→TAG) | yhaV → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,102,851 | 0 | G | A | 8.6% | 178.6 / 6.7 | 58 | W143* (TGG→TAG) | yhaV | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (24/29); new base A (3/2); total (27/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
ACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATT > minE/2102782‑2102917 | aCGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTgg > 1:2423373/1‑71 (MQ=255) tAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa < 1:601298/70‑1 (MQ=255) tAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa < 1:1553830/70‑1 (MQ=255) tAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa < 1:2758825/70‑1 (MQ=255) aaaaaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAc > 1:204795/1‑70 (MQ=255) aaaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt > 1:252492/1‑71 (MQ=255) aaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa < 1:542023/65‑1 (MQ=255) aaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa < 1:1883186/65‑1 (MQ=255) aaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaa < 1:2995421/65‑1 (MQ=255) gATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCg < 1:1965871/71‑1 (MQ=255) taCCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaaa > 1:1557748/1‑68 (MQ=255) aCCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaaa > 1:1155165/1‑52 (MQ=255) aCCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaaa > 1:2448683/1‑52 (MQ=255) tATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt < 1:566815/52‑1 (MQ=255) tATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt < 1:215711/52‑1 (MQ=255) ttCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaaa < 1:1331427/46‑1 (MQ=255) ttCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaaa < 1:880503/46‑1 (MQ=255) ttCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAc > 1:1451344/1‑71 (MQ=255) ttCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAc > 1:2378928/1‑71 (MQ=255) ttCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAc > 1:1932497/1‑71 (MQ=255) aGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAccc < 1:1069744/70‑1 (MQ=255) aGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAccc < 1:678934/70‑1 (MQ=255) gCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTAGGAAACCCTCACCCGAGAAACag < 1:772152/60‑1 (MQ=255) cAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAtt < 1:528949/71‑1 (MQ=255) aaaaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAtt < 1:2212097/70‑1 (MQ=255) aaaaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAt > 1:1489631/1‑69 (MQ=255) aaaaTGTTAAAAAGAGGACATCCTCCTGCCGACTAGGAAACCCTCACCCGAGAAACagaa > 1:481074/1‑60 (MQ=255) aaaaTGTTAAAAAGAGGACATCCTCCTGCCGACTAGGAAACCCTCACCCGAGAAACagaa > 1:826017/1‑60 (MQ=255) aaaaTGTTAAAAAGAGGACATCCTCCTGCCGACTAGGAAACCCTCACCCGAGAAACagaa > 1:2743748/1‑60 (MQ=255) aaaTGTTAAAAAGAGGACATCCTCCTGCCGACTgg > 1:73491/1‑35 (MQ=255) aaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGa < 1:2726665/70‑1 (MQ=255) aTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATg > 1:1496259/1‑71 (MQ=255) tGTTAAAAAGAGGACATCCTCCTGCCGACTAGGAAACCCTCACCCGAGAAACag < 1:2891295/54‑1 (MQ=255) ttAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtg > 1:2699562/1‑71 (MQ=255) ttAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtg < 1:798054/71‑1 (MQ=255) ttAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtg > 1:2234209/1‑71 (MQ=255) ttAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATCgtg > 1:2703592/1‑71 (MQ=255) tAAAAAGAGGACATCCTCCTGCCGACTGGG‑AACCCTCACCCGAGAAACaga > 1:799881/1‑51 (MQ=255) aaaaaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGctgt < 1:1065803/70‑1 (MQ=255) aaaaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCAt < 1:2331928/60‑1 (MQ=255) aaaaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGtt < 1:917087/70‑1 (MQ=255) aaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTcac < 1:3408026/71‑1 (MQ=255) aaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTcac < 1:1917132/71‑1 (MQ=255) gACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGc < 1:3032344/71‑1 (MQ=255) gACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGc < 1:2531292/71‑1 (MQ=255) cATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGccc > 1:2255891/1‑71 (MQ=255) cATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGccc > 1:1796030/1‑71 (MQ=255) aTCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACag < 1:473187/39‑1 (MQ=255) aTCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccg > 1:2957786/1‑71 (MQ=255) tcctccTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccgc > 1:3024110/1‑71 (MQ=255) tcctccTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccgc > 1:539575/1‑71 (MQ=255) tcctccTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccgc > 1:1137785/1‑71 (MQ=255) ctcctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccgccg < 1:1156734/71‑1 (MQ=255) tcctGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccgccg > 1:611167/1‑70 (MQ=255) tGCCGACTGGGAAACCCTCACCCGAGAAACagaag > 1:1908798/1‑35 (MQ=255) tGCCGACTGGGAAACCCTCACCCGAGAAACagaag > 1:18188/1‑35 (MQ=255) ccGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACg < 1:654688/69‑1 (MQ=255) ccGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACg < 1:3114121/69‑1 (MQ=255) cGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt < 1:1090713/71‑1 (MQ=255) gACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAtt > 1:196534/1‑71 (MQ=255) | ACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATT > minE/2102782‑2102917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |