| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,254,635 | A→C | 9.8% | V801V (GTT→GTG) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,254,635 | 0 | A | C | 9.8% | 255.2 / 20.6 | 92 | V801V (GTT→GTG) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (33/50); new base C (3/6); total (36/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGAGTCATCATGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCAC > minE/2254567‑2254705 | ggAGTCATCATGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAAc < 1:95799/71‑1 (MQ=255)ggAGTCATCATGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAAc < 1:3140067/71‑1 (MQ=255)ggAGTCATCATGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAAc < 1:3059152/71‑1 (MQ=255) gAGTCATCATGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAAcc < 1:2241685/71‑1 (MQ=255) tcatGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAgacga > 1:933175/1‑71 (MQ=255) atGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAgacgac > 1:2681904/1‑70 (MQ=255) atGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGacgacg < 1:2885991/71‑1 (MQ=255) atGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGacgacg < 1:617799/71‑1 (MQ=255) atGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGacgacg < 1:29454/71‑1 (MQ=255) tGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAgac < 1:2115601/66‑1 (MQ=255) tGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAgac < 1:3021307/66‑1 (MQ=255) tGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAgac < 1:3220793/66‑1 (MQ=255) aTACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAg > 1:1895339/1‑70 (MQ=255) aTACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGt > 1:1057772/1‑71 (MQ=255) aTACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGt > 1:368078/1‑71 (MQ=255) tACCTACATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGt > 1:1361052/1‑70 (MQ=255) tCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGAcgacga > 1:3122633/1‑63 (MQ=255) tCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGAcgacga > 1:3084721/1‑63 (MQ=255) tCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGAcgacga > 1:1817848/1‑63 (MQ=255) tCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGt > 1:1600744/1‑70 (MQ=255) tCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGt > 1:2967108/1‑70 (MQ=255) cATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGt < 1:3270563/69‑1 (MQ=255) cATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGAAGAGTCAGGTa < 1:714221/70‑1 (MQ=255) aTGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAAc < 1:1723953/71‑1 (MQ=255) aCCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGt > 1:1814300/1‑71 (MQ=255) ccACAATCGTCTTCGGTAACCACCAGGTTCTGCGCCACGGCAACCAGACGACGAGTCAGGTAACCGGAGt < 1:478107/70‑1 (MQ=255) cacaATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAAc < 1:3060457/43‑1 (MQ=255) cacaATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTc > 1:2811659/1‑71 (MQ=255) caATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCg > 1:2517912/1‑70 (MQ=255) aaTCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTc < 1:2761326/53‑1 (MQ=255) aTCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTa < 1:1391619/57‑1 (MQ=255) aTCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTa < 1:411812/57‑1 (MQ=255) aTCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAg < 1:3231330/71‑1 (MQ=255) aTCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAg < 1:746673/71‑1 (MQ=255) tCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGt > 1:1741901/1‑71 (MQ=255) tCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGt > 1:2539298/1‑71 (MQ=255) tCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTa < 1:145764/53‑1 (MQ=255) tCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGtttt < 1:1208562/71‑1 (MQ=255) tCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGtttt < 1:3479414/71‑1 (MQ=255) ttCGGTAACCACCAGGTCCTGCGCCACGTCAACCAgacgac > 1:2283414/1‑41 (MQ=255) ttCGGTAACCACCAGGTCCTGCGCCACGTCAACCAgacgac > 1:427744/1‑41 (MQ=255) ttCGGTAACCACCAGGTCCTGCGCCACGTCAACCAgacgac > 1:2151196/1‑41 (MQ=255) ttCGGTAACCACCAGGTCCTGCGCCACGTCAACCAgacgac > 1:454034/1‑41 (MQ=255) ttCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAAc > 1:281974/1‑53 (MQ=255) cGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAg > 1:1319297/1‑46 (MQ=255) gTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTg < 1:846624/70‑1 (MQ=255) gTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTg < 1:2128214/70‑1 (MQ=255) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:3434053/39‑1 (MQ=16) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:606162/39‑1 (MQ=16) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:196598/39‑1 (MQ=16) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:3329271/39‑1 (MQ=16) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:691904/39‑1 (MQ=16) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:801822/39‑1 (MQ=16) tAACCACCAGGTCCTGCGCCACGTCAACCAGAcgacga < 1:428827/38‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:2749328/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:1200365/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:2208723/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:1947422/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:2330950/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:2366973/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCg > 1:762294/1‑71 (MQ=255) accaccAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:660541/49‑1 (MQ=255) accaccAGATCTTGCGTCACGTCCACCAGACGACGGGTCAg > 1:1191944/1‑41 (MQ=19) accaccAGATCTTGCGTCACGTCCACCAGACGACGGGTCAg > 1:2577199/1‑41 (MQ=19) accaccAGATCTTGCGTCACGTCCACCAGACGACGGGTCAg > 1:2836454/1‑41 (MQ=19) gTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGtttt < 1:3193477/54‑1 (MQ=255) gTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCa < 1:1632490/71‑1 (MQ=255) gTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCa < 1:1529348/71‑1 (MQ=255) gTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCa < 1:2112265/71‑1 (MQ=255) cgcCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGa > 1:1208169/1‑67 (MQ=255) cgcCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGAc > 1:2045586/1‑68 (MQ=255) cgcCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGAc > 1:1407179/1‑68 (MQ=255) cgcCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGAc > 1:2030528/1‑68 (MQ=255) cgcCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCtt < 1:2442844/71‑1 (MQ=255) ccACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGtttt < 1:2868052/46‑1 (MQ=255) ccACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGtttt < 1:2858556/46‑1 (MQ=255) ccACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGttt < 1:3264060/45‑1 (MQ=255) ccACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGttt < 1:3006562/45‑1 (MQ=255) cACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTAc < 1:3055801/71‑1 (MQ=255) aCGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTc > 1:943406/1‑45 (MQ=255) aCGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTc > 1:1068827/1‑45 (MQ=255) aCGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTc > 1:1354788/1‑45 (MQ=255) aCGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCa > 1:279901/1‑46 (MQ=255) aCGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTa > 1:218428/1‑69 (MQ=255) cGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCttt < 1:2718938/67‑1 (MQ=255) cGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCttt < 1:267509/67‑1 (MQ=255) cGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCttt < 1:2648786/67‑1 (MQ=255) cGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCttt < 1:1171308/67‑1 (MQ=255) cGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACg > 1:1078566/1‑70 (MQ=255) gTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCttt < 1:1302728/66‑1 (MQ=255) tCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGttt < 1:1169482/40‑1 (MQ=255) tCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTc < 1:163596/42‑1 (MQ=255) aaCCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCAc > 1:1027300/1‑71 (MQ=255) | GGAGTCATCATGATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCAC > minE/2254567‑2254705 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |