| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,254,647 | A→G | 13.5% | T797T (ACT→ACC) | rpoC ← | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,254,647 | 0 | A | G | 13.5% | 256.6 / 25.2 | 89 | T797T (ACT→ACC) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base A (35/42); new base G (3/9); total (38/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCGTGGGTG > minE/2254579‑2254713 | aTACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAg > 1:1895339/1‑70 (MQ=255)aTACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGt > 1:1057772/1‑71 (MQ=255)aTACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGt > 1:368078/1‑71 (MQ=255) tACCTACATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGt > 1:1361052/1‑70 (MQ=255) tCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGAcgacga > 1:1817848/1‑63 (MQ=255) tCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGAcgacga > 1:3122633/1‑63 (MQ=255) tCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGAcgacga > 1:3084721/1‑63 (MQ=255) tCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGt > 1:2967108/1‑70 (MQ=255) tCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGt > 1:1600744/1‑70 (MQ=255) cATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGt < 1:3270563/69‑1 (MQ=255) cATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGAAGAGTCAGGTa < 1:714221/70‑1 (MQ=255) aTGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAAc < 1:1723953/71‑1 (MQ=255) aCCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGt > 1:1814300/1‑71 (MQ=255) ccACAATCGTCTTCGGTAACCACCAGGTTCTGCGCCACGGCAACCAGACGACGAGTCAGGTAACCGGAGt < 1:478107/70‑1 (MQ=255) cacaATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTc > 1:2811659/1‑71 (MQ=255) caATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCg > 1:2517912/1‑70 (MQ=255) aaTCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTc < 1:2761326/53‑1 (MQ=255) aTCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTa < 1:411812/57‑1 (MQ=255) aTCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTa < 1:1391619/57‑1 (MQ=255) aTCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAg < 1:3231330/71‑1 (MQ=255) aTCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAg < 1:746673/71‑1 (MQ=255) tCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGt > 1:2539298/1‑71 (MQ=255) tCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGt > 1:1741901/1‑71 (MQ=255) tCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTa < 1:145764/53‑1 (MQ=255) tCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGtttt < 1:3479414/71‑1 (MQ=255) tCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGtttt < 1:1208562/71‑1 (MQ=255) ttCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAAc > 1:281974/1‑53 (MQ=255) cGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAg > 1:1319297/1‑46 (MQ=255) gTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTg < 1:2128214/70‑1 (MQ=255) gTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTg < 1:846624/70‑1 (MQ=255) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:606162/39‑1 (MQ=16) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:196598/39‑1 (MQ=16) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:691904/39‑1 (MQ=16) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:3434053/39‑1 (MQ=16) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:801822/39‑1 (MQ=16) tcaccaccAGATCTTGCGTCACGTCCACCAGACGACGGGTc < 1:3329271/39‑1 (MQ=16) tAACCACCAGGTCCTGCGCCACGTCAACCAGAcgacga < 1:428827/38‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:2749328/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:2366973/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:2330950/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:1947422/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:2208723/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:1200365/51‑1 (MQ=255) tAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCg > 1:762294/1‑71 (MQ=255) accaccAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCgg < 1:660541/49‑1 (MQ=255) accaccAGATCTTGCGTCACGTCCACCAGACGACGGGTCAg > 1:1191944/1‑41 (MQ=19) accaccAGATCTTGCGTCACGTCCACCAGACGACGGGTCAg > 1:2836454/1‑41 (MQ=19) accaccAGATCTTGCGTCACGTCCACCAGACGACGGGTCAg > 1:2577199/1‑41 (MQ=19) gTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGtttt < 1:3193477/54‑1 (MQ=255) gTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCa < 1:1632490/71‑1 (MQ=255) gTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCa < 1:1529348/71‑1 (MQ=255) gTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCa < 1:2112265/71‑1 (MQ=255) cgcCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGa > 1:1208169/1‑67 (MQ=255) cgcCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGAc > 1:1407179/1‑68 (MQ=255) cgcCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGAc > 1:2030528/1‑68 (MQ=255) cgcCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGAc > 1:2045586/1‑68 (MQ=255) cgcCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCtt < 1:2442844/71‑1 (MQ=255) ccACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGtttt < 1:2868052/46‑1 (MQ=255) ccACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGtttt < 1:2858556/46‑1 (MQ=255) ccACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGttt < 1:3006562/45‑1 (MQ=255) ccACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGttt < 1:3264060/45‑1 (MQ=255) cACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTAc < 1:3055801/71‑1 (MQ=255) aCGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTc > 1:943406/1‑45 (MQ=255) aCGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTc > 1:1354788/1‑45 (MQ=255) aCGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTc > 1:1068827/1‑45 (MQ=255) aCGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCa > 1:279901/1‑46 (MQ=255) aCGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTa > 1:218428/1‑69 (MQ=255) cGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCttt < 1:267509/67‑1 (MQ=255) cGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCttt < 1:2648786/67‑1 (MQ=255) cGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCttt < 1:2718938/67‑1 (MQ=255) cGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCttt < 1:1171308/67‑1 (MQ=255) cGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACg > 1:1078566/1‑70 (MQ=255) gTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCttt < 1:1302728/66‑1 (MQ=255) tCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGttt < 1:1169482/40‑1 (MQ=255) tCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTc < 1:163596/42‑1 (MQ=255) aaCCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCAc > 1:1027300/1‑71 (MQ=255) aCCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCAcc > 1:1679151/1‑71 (MQ=255) aCCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCAcc > 1:555729/1‑71 (MQ=255) aCCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCAcc > 1:1608306/1‑71 (MQ=255) aCCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACAAGCAcc > 1:2003846/1‑71 (MQ=255) ccAGACGACGGGTCAGGTAACCCGAGTTCGCGGTCTTCAGCGCCGTATCCGCCAGACCTTTACGAGCGCCg < 1:2221972/71‑1 (MQ=255) ccAGACGACGGGTCAGGTAACCCGAGTTCGCGGTCTTCAGCGCCGTATCCGCCAGACCTTTACGAGCGCCg < 1:2281818/71‑1 (MQ=255) ccAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTc < 1:3292688/38‑1 (MQ=255) ccAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTc < 1:634127/38‑1 (MQ=255) ccAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCAcc > 1:2607307/1‑70 (MQ=255) ccAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCg > 1:1747440/1‑71 (MQ=255) cAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCtt < 1:2052106/59‑1 (MQ=255) cAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCgt > 1:1785586/1‑71 (MQ=255) aGACGACGGGTCAGGTAACCCGAGTTCGCGGTCTTCAGCGCCGTATCCGCCAGACCTTTACGAGCGCCg < 1:2369359/69‑1 (MQ=255) aGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCAcc > 1:1043161/1‑68 (MQ=255) aGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCgtg > 1:3337733/1‑71 (MQ=255) gacgacGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTg < 1:3069493/39‑1 (MQ=255) gacgacGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCgtgg > 1:283928/1‑71 (MQ=255) gaGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCgtgggtg > 1:2829276/1‑68 (MQ=255) gaGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCgtggg > 1:390718/1‑66 (MQ=255) gaGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCgtggg > 1:921506/1‑66 (MQ=255) | ATACCTTCATGGGTACCACAATCGTCTTCGGTAACCACCAGGTCCTGCGCCACGTCAACCAGACGACGAGTCAGGTAACCGGAGTTCGCAGTTTTCAGTGCGGTATCCGCCAGACCTTTACGAGCACCGTGGGTG > minE/2254579‑2254713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |