| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,469,888 | T→G | 15.2% | S290S (TCT→TCG) | atpD → | F1 sector of membrane‑bound ATP synthase, beta subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,469,888 | 0 | T | G | 15.2% | 172.0 / 33.2 | 79 | S290S (TCT→TCG) | atpD | F1 sector of membrane‑bound ATP synthase, beta subunit |
| Reads supporting (aligned to +/- strand): ref base T (43/24); new base G (5/7); total (48/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GTTATCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTT > minE/2469818‑2469956 | gTTATCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCt < 1:2340304/71‑1 (MQ=255) tATCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTa > 1:3183917/1‑70 (MQ=255) aTCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTAt < 1:1744570/70‑1 (MQ=255) aTCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTAt > 1:2043747/1‑70 (MQ=255) tCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCa > 1:3226352/1‑71 (MQ=255) cAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCAc > 1:3237604/1‑71 (MQ=255) cGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTcc > 1:3212824/1‑71 (MQ=255) cTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTa < 1:2875385/58‑1 (MQ=255) cTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTAt > 1:1567215/1‑59 (MQ=255) cTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTa > 1:1963583/1‑69 (MQ=255) ggCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATc > 1:2466996/1‑58 (MQ=255) ggCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATc > 1:2211391/1‑58 (MQ=255) ggCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATc > 1:415046/1‑58 (MQ=255) ggAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg < 1:1257744/71‑1 (MQ=255) gAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg < 1:1887403/70‑1 (MQ=255) aaGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta < 1:861823/71‑1 (MQ=255) agagATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta < 1:1806405/70‑1 (MQ=255) gagaTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCt > 1:829358/1‑48 (MQ=255) gagaTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCt > 1:1956310/1‑48 (MQ=255) gagaTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCt > 1:1888344/1‑48 (MQ=255) aTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACg > 1:3367624/1‑70 (MQ=255) aTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACg > 1:2949495/1‑70 (MQ=255) aTGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACg > 1:277513/1‑70 (MQ=255) gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa > 1:2234281/1‑55 (MQ=255) gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa > 1:2833313/1‑55 (MQ=255) gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa > 1:98740/1‑55 (MQ=255) gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa > 1:3231985/1‑55 (MQ=255) gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa > 1:1205519/1‑55 (MQ=255) gCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACa > 1:622869/1‑55 (MQ=255) cGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCt > 1:1155231/1‑70 (MQ=255) cGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTg > 1:2782294/1‑71 (MQ=255) ttCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCg < 1:3401195/71‑1 (MQ=255) ttCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCg < 1:3397186/71‑1 (MQ=255) cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg < 1:2051383/55‑1 (MQ=255) cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg < 1:1209989/55‑1 (MQ=255) cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAg < 1:1734919/55‑1 (MQ=255) cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGa > 1:2044478/1‑71 (MQ=255) cTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGa > 1:1775969/1‑71 (MQ=255) gCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta > 1:3258571/1‑55 (MQ=255) cAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAt < 1:602824/69‑1 (MQ=255) gAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgactt < 1:3045456/71‑1 (MQ=255) gAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgactt < 1:30089/71‑1 (MQ=255) gAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgactt < 1:1441752/71‑1 (MQ=255) gTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttgact < 1:1575890/71‑1 (MQ=255) gTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttga > 1:830049/1‑69 (MQ=255) tATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttgact < 1:2947086/70‑1 (MQ=255) tATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttgact < 1:2384686/70‑1 (MQ=255) aTCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTtgactga > 1:1691340/1‑71 (MQ=255) tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc < 1:2837128/51‑3 (MQ=255) tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc < 1:1522286/51‑3 (MQ=255) tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc < 1:357078/51‑3 (MQ=255) tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc < 1:2273389/51‑3 (MQ=255) tCACCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGttc < 1:2320244/51‑3 (MQ=255) tCACCTCCACCAAAACTGGTTCTATCACCTCCGTa < 1:2545540/35‑1 (MQ=255) tCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTtgactgac > 1:101238/1‑71 (MQ=255) cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGtat > 1:1618831/1‑44 (MQ=255) cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGtat > 1:3376782/1‑44 (MQ=255) cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGta > 1:1589875/1‑43 (MQ=255) cACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGAcc > 1:904085/1‑71 (MQ=255) cACCTCAACCAAAACTGGTTCTATCACCTCCGTACAGGCAGtat > 1:1853/1‑44 (MQ=255) aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc > 1:807223/1‑50 (MQ=255) aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc > 1:1456628/1‑50 (MQ=255) aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc > 1:1656986/1‑50 (MQ=255) aCCTCCACCAAGAAAGGCTCGATCACCTCGATCCAGGCCGTATACGTTccc > 1:1711370/1‑50 (MQ=255) aCCTCCACCAAGAAAGGCTCGATCACCTCGAACCAGGCCGTATACGttc > 1:230767/1‑47 (MQ=255) aCCTCCACCAAAACTGGTTCTATCACCTCCGTACa > 1:902396/1‑35 (MQ=255) aCCTCCACCAAAACTGGTTCTATCACCTCCGTACa > 1:1177211/1‑35 (MQ=255) ccTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTAc > 1:847435/1‑48 (MQ=255) ccTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCg < 1:1487376/71‑1 (MQ=255) cTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGt < 1:939294/71‑1 (MQ=255) caccaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGtctc > 1:2021175/1‑71 (MQ=255) caccaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGtctc > 1:521784/1‑71 (MQ=255) accaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTcc > 1:854831/1‑71 (MQ=255) accaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTcc > 1:1149309/1‑71 (MQ=255) accaAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTcc > 1:1484170/1‑71 (MQ=255) aaaaCTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCgg > 1:2002623/1‑70 (MQ=255) aaaaCTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGc < 1:493031/71‑1 (MQ=255) aaCTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGAtgacttga > 1:56397/1‑53 (MQ=255) ccggTTCGATCACCTCGATCCAGGCCGTCTACTTCCCTGCGGATGACTTGACCGACCCGTCGCCTGCAacc < 1:308413/69‑1 (MQ=255) ccggTTCGATCACCTCGATCCAGGCCGTCTACGTCCCTGCGGATGACTTGACCGACCCGTCGCCTGCAacc < 1:2660082/69‑1 (MQ=255) ttCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCt < 1:1712074/71‑1 (MQ=255) ttCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCt < 1:2206227/71‑1 (MQ=255) tCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCtt > 1:766038/1‑71 (MQ=255) | GTTATCAGCCGACCCTGGCGGAAGAGATGGGCGTTCTGCAGGAACGTATCACCTCCACCAAAACTGGTTCTATCACCTCCGTACAGGCAGTATACGTACCTGCGGATGACTTGACTGACCCGTCTCCGGCAACCACCTT > minE/2469818‑2469956 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |