| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,682,335 | T→C | 11.6% | K459K (AAA→AAG) | yhgF ← | predicted transcriptional accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,682,335 | 0 | T | C | 11.6% | 123.5 / 7.4 | 43 | K459K (AAA→AAG) | yhgF | predicted transcriptional accessory protein |
| Reads supporting (aligned to +/- strand): ref base T (23/15); new base C (2/3); total (25/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
GTTTGCGGGCCAGTTGCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTC > minE/2682265‑2682403 | gTTTGCGGGCCAGTTGCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGAtt < 1:2606632/71‑1 (MQ=255)gTTTGCGGGCCAGTTGCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGAtt < 1:2728658/71‑1 (MQ=255)gTTTGCGGGCCAGTTGCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGAtt < 1:3213142/71‑1 (MQ=255) tGCGGGCCAGTTGCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGAtttt > 1:741153/1‑70 (MQ=255) gCGGGCCAGTTGCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTc < 1:1801006/70‑1 (MQ=255) gggCCAGTTGCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCAcc < 1:2251506/71‑1 (MQ=255) gCCAGTTGCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAg > 1:1933332/1‑71 (MQ=255) gCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGAtt > 1:558308/1‑56 (MQ=255) gCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGAtt > 1:64617/1‑56 (MQ=255) gCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGc > 1:2962185/1‑70 (MQ=255) gCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGc > 1:1841534/1‑70 (MQ=255) gCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGc > 1:188681/1‑70 (MQ=255) gCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGc < 1:2697400/70‑1 (MQ=255) cTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTc > 1:3309793/1‑63 (MQ=255) cTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCg > 1:2433256/1‑71 (MQ=255) tGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCg > 1:1707292/1‑70 (MQ=255) tGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCg > 1:2579544/1‑70 (MQ=255) gCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGa < 1:3081906/70‑1 (MQ=255) cTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATc > 1:1913189/1‑71 (MQ=255) aCGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTg > 1:3245242/1‑71 (MQ=255) tCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCa > 1:1298807/1‑70 (MQ=255) aTGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCaaa < 1:2282698/70‑1 (MQ=255) aTGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCaaa < 1:1198282/70‑1 (MQ=255) aTGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAAc > 1:676111/1‑71 (MQ=255) gCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCTCCAg > 1:2840550/1‑46 (MQ=255) gCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAg > 1:228192/1‑46 (MQ=255) gCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAg > 1:478935/1‑46 (MQ=255) gCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAg > 1:1761845/1‑46 (MQ=255) cTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGa < 1:1400733/58‑1 (MQ=255) cTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAAcggc > 1:2338258/1‑71 (MQ=255) cTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAAcggc > 1:1821034/1‑71 (MQ=255) aTACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAAcggcg < 1:1752186/69‑1 (MQ=255) tACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACggcgggc < 1:817519/71‑1 (MQ=255) tACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACggcgggc > 1:2055915/1‑71 (MQ=255) cTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACGGCGGGCGa < 1:3139245/71‑1 (MQ=255) cTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACGGCGGGCGa < 1:1481402/71‑1 (MQ=255) tGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACGGCGGGCGa > 1:1837115/1‑70 (MQ=255) aCGCCGATCGACTTCGGATCGATCTTGACCAGTTCGGCCAGCGGATcc < 1:3331199/48‑1 (MQ=19) aCGCCGATCGACTTCGGATCGATCTTGACCAGTTCGGCCAGCGGATcc < 1:2108225/48‑1 (MQ=19) aCGCCGATCGACTTCGGATCGATCTTGACCAGTTCGGCCAGCGGATcc < 1:2169859/48‑1 (MQ=19) cGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACGGCGGGCGATAGacac < 1:54263/70‑1 (MQ=255) tAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTg > 1:1959605/1‑43 (MQ=255) tAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACGGCGGGCGATAGACACCGCGcc < 1:679028/70‑1 (MQ=255) cGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGc > 1:3388262/1‑70 (MQ=255) cGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACAGCGGGCGATAGACACCGCGCCACGCAGc > 1:1670262/1‑70 (MQ=255) ggATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGa < 1:626333/71‑1 (MQ=255) gATCTTCACCAGCTCCGCCAGCGGGTCCTGCAGGCGACGGGCTATGGATACGGCGCCGCGCAGGGACACGt > 1:1357987/1‑71 (MQ=255) gATCTTCACCAGCTCCGCCAGCGGGTCCTGCAGGCGACGGGCGATGGATACGGCGCCGCGCAGGGACACGt > 1:55007/1‑71 (MQ=255) aTTTTCACCAGCTCCGCCAGCGGATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTc < 1:637323/71‑1 (MQ=255) | GTTTGCGGGCCAGTTGCGTCTGGCTGACGTCATGCTGATACTGACCTACGCCGATAGATTTCGGATCGATTTTCACCAGCTCCGCCAGCGGATCCTGCAAACGGCGGGCGATAGACACCGCGCCACGCAGCGAAACGTC > minE/2682265‑2682403 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |