| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,811,198 | A→C | 8.2% | S516S (TCT→TCG) | uvrA ← | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,811,198 | 0 | A | C | 8.2% | 130.8 / 6.9 | 49 | S516S (TCT→TCG) | uvrA | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
| Reads supporting (aligned to +/- strand): ref base A (15/30); new base C (2/2); total (17/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
TATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCT > minE/2811128‑2811268 | tATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATa < 1:1438171/71‑1 (MQ=255) aCCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACg > 1:1063509/1‑71 (MQ=255) aCCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACg > 1:2099303/1‑71 (MQ=255) ccGAGATCGCGCAGATGGATAAGCGTACCCGACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACg < 1:1106962/70‑1 (MQ=255) cGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGc > 1:478033/1‑71 (MQ=255) cGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGc > 1:1856650/1‑71 (MQ=255) tCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTc < 1:2502098/71‑1 (MQ=255) tCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTc < 1:1628141/71‑1 (MQ=255) gcgcAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCa < 1:836631/71‑1 (MQ=255) cgcAGATGGATAAGCGTACCCGACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCa < 1:2426505/70‑1 (MQ=255) gcAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAg > 1:1540527/1‑70 (MQ=255) gcAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAg > 1:1661941/1‑70 (MQ=255) aTGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGt < 1:2888431/71‑1 (MQ=255) tGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTa < 1:2029792/71‑1 (MQ=255) ggATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACg < 1:106866/68‑1 (MQ=255) ggATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTa > 1:236746/1‑70 (MQ=255) aTAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCa < 1:2037376/61‑1 (MQ=255) aTAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCa < 1:819824/61‑1 (MQ=255) aTAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCAAGCACGTACAt < 1:1926484/71‑1 (MQ=255) tAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAg < 1:1564651/61‑1 (MQ=255) tAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAg < 1:2574664/61‑1 (MQ=255) tAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACaag < 1:1868210/71‑3 (MQ=255) aaGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCGGc > 1:350145/1‑61 (MQ=255) aaGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGc > 1:393890/1‑61 (MQ=255) aaGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGc > 1:2436471/1‑61 (MQ=255) aGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACaaa > 1:611333/1‑67 (MQ=255) aGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATa > 1:2187405/1‑69 (MQ=255) gCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACg < 1:166492/71‑1 (MQ=255) tACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCa < 1:470869/71‑1 (MQ=255) ccAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAAcc < 1:42827/71‑1 (MQ=255) aCAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGt < 1:2855466/54‑1 (MQ=255) aGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCa > 1:1843768/1‑49 (MQ=255) tCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCAc > 1:2320166/1‑70 (MQ=255) gTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCa < 1:3226517/70‑1 (MQ=255) ttATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCa < 1:3212720/69‑1 (MQ=255) tcgcGCTGGTGCAGGCCGATCGACGGTTCGTCGAGCACGTACATGACGCCGGTCAGGCCCGAACCGATCt < 1:2440271/67‑1 (MQ=255) tcgcGCTGGTGCAGGCCGATCGACGGTTCGTCGAGCACGTACATGACGCCGGTCAGGCCCGAACCGATCt < 1:3387150/67‑1 (MQ=255) aCGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTgg < 1:1199231/70‑1 (MQ=255) aCGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTgg < 1:723193/70‑1 (MQ=255) cGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACAt < 1:1271492/41‑1 (MQ=255) cTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCg < 1:3408779/71‑1 (MQ=255) cTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCg < 1:1128334/71‑1 (MQ=255) cTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCg < 1:1273995/71‑1 (MQ=255) gTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCa < 1:804620/71‑1 (MQ=255) gTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCa < 1:98131/71‑1 (MQ=255) gCAGGCCGCTCGATGGCTCGTCCAGCACGTAGACCACCCCGAACAGGCTCGATCGCACCTGGGTCGCCAgg > 1:2624099/1‑70 (MQ=255) gCAGGCCGCTCGATGGCTCGTCCAGCACGTAGACCACCCCGAACAGGCTCGATCGCACCTGGGTCGCCAg > 1:1541802/1‑70 (MQ=255) cAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCt > 1:1241938/1‑59 (MQ=255) aGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACg < 1:2650674/71‑1 (MQ=255) ggCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACgg < 1:3034376/71‑1 (MQ=255) aGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCt > 1:3172595/1‑71 (MQ=255) | TATTACCGAGATCGCGCAGATGGATAAGCGTACCCAACAGGCGCTCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCT > minE/2811128‑2811268 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |