| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,811,239 | C→A | 6.9% | A503S (GCG→TCG) | uvrA ← | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,811,239 | 0 | C | A | 6.9% | 203.4 / 2.2 | 58 | A503S (GCG→TCG) | uvrA | ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC |
| Reads supporting (aligned to +/- strand): ref base C (31/23); new base A (2/2); total (33/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
TCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGAAAGC > minE/2811172‑2811309 | tCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCAc > 1:2320166/1‑70 (MQ=255) gTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCa < 1:3226517/70‑1 (MQ=255) ttATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCa < 1:3212720/69‑1 (MQ=255) tcgcGCTGGTGCAGGCCGATCGACGGTTCGTCGAGCACGTACATGACGCCGGTCAGGCCCGAACCGATCt < 1:2440271/67‑1 (MQ=255) tcgcGCTGGTGCAGGCCGATCGACGGTTCGTCGAGCACGTACATGACGCCGGTCAGGCCCGAACCGATCt < 1:3387150/67‑1 (MQ=255) aCGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTgg < 1:1199231/70‑1 (MQ=255) aCGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTgg < 1:723193/70‑1 (MQ=255) cTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCg < 1:3408779/71‑1 (MQ=255) cTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCg < 1:1128334/71‑1 (MQ=255) cTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCg < 1:1273995/71‑1 (MQ=255) gTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCa < 1:98131/71‑1 (MQ=255) gTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCa < 1:804620/71‑1 (MQ=255) gCAGGCCGCTCGATGGCTCGTCCAGCACGTAGACCACCCCGAACAGGCTCGATCGCACCTGGGTCGCCAgg > 1:2624099/1‑70 (MQ=255) gCAGGCCGCTCGATGGCTCGTCCAGCACGTAGACCACCCCGAACAGGCTCGATCGCACCTGGGTCGCCAg > 1:1541802/1‑70 (MQ=255) cAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCt > 1:1241938/1‑59 (MQ=255) aGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACg < 1:2650674/71‑1 (MQ=255) ggCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACgg < 1:3034376/71‑1 (MQ=255) aGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCt > 1:3172595/1‑71 (MQ=255) gACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtg > 1:2748485/1‑71 (MQ=255) gACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtg > 1:815522/1‑71 (MQ=255) gACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtg < 1:2722402/71‑1 (MQ=255) aCGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTc > 1:510876/1‑53 (MQ=255) aCGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTc > 1:1874861/1‑53 (MQ=255) aCGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTc > 1:146977/1‑53 (MQ=255) aCGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtgt > 1:1998867/1‑71 (MQ=255) aCGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtgt > 1:2401672/1‑71 (MQ=255) cGGCTCGTCCAGCACGTACATAACGCCAACCAG‑‑‑‑GCACCAATCTGGCTCGCCAGACGGATACGCTGTGCtt > 1:1068363/1‑70 (MQ=255) tCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCAcc < 1:647019/71‑1 (MQ=255) ccAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCg > 1:382028/1‑71 (MQ=255) ccAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCg > 1:2308063/1‑71 (MQ=255) ccAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCg > 1:1860411/1‑71 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATc > 1:253769/1‑37 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATc > 1:1022847/1‑37 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATc > 1:1093219/1‑37 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATc > 1:3277001/1‑37 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATc > 1:1100617/1‑37 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATc > 1:145387/1‑37 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATc > 1:1864661/1‑37 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATc > 1:2424079/1‑37 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATc > 1:2440669/1‑37 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATc > 1:524372/1‑37 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCa > 1:2887689/1‑67 (MQ=255) cAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGc > 1:1870083/1‑71 (MQ=255) cGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGaa < 1:1104057/71‑1 (MQ=255) gTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGaaa < 1:2532710/71‑1 (MQ=255) tACATAACGCCAACCAGGCCCGCACCAATCTGGCTCg < 1:444979/37‑1 (MQ=255) tACATAACGCCAACCAGGCCCGCACCAATCTGGCTCg < 1:99193/37‑1 (MQ=255) tACATAACGCCAACCAGGCCCGCACCAATCTGGCTCg < 1:2130111/37‑1 (MQ=255) tACATAACGCCAACCAGGCCCGCACCAATCTGGCTCg < 1:1083039/37‑1 (MQ=255) cATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGa < 1:2541613/44‑1 (MQ=255) cATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGa < 1:1914330/44‑1 (MQ=255) tAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCtgtg < 1:1037684/51‑1 (MQ=255) aCGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCt > 1:3333134/1‑51 (MQ=255) aCGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCt > 1:2970702/1‑51 (MQ=255) aCGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCt > 1:775096/1‑51 (MQ=255) ccAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAg > 1:2097285/1‑71 (MQ=255) ccGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGaa < 1:455901/71‑1 (MQ=255) cGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGa > 1:848252/1‑69 (MQ=255) cACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGAAAGc < 1:2416928/71‑1 (MQ=255) | TCGTTATCACGCTGGTGCAGGCCGATAGACGGCTCGTCCAGCACGTACATAACGCCAACCAGGCCCGCACCAATCTGGCTCGCCAGACGGATACGCTGTGCTTCACCGCCAGAAAGCGTTTCTGCCGAGCGGGAAAGC > minE/2811172‑2811309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |