| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 394,053 | T→C | 7.0% | V71A (GTA→GCA) | ahpC → | alkyl hydroperoxide reductase, C22 subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 394,053 | 0 | T | C | 7.0% | 215.7 / 6.1 | 71 | V71A (GTA→GCA) | ahpC | alkyl hydroperoxide reductase, C22 subunit |
| Reads supporting (aligned to +/- strand): ref base T (23/43); new base C (2/3); total (25/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
CCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAA > minE/393983‑394120 | ccgaccgaACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGt < 1:2693110/71‑1 (MQ=255)ccgaccgaACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGt < 1:503194/71‑1 (MQ=255) aaCTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTAcc < 1:2077611/71‑1 (MQ=255) aCTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTAcc > 1:2485556/1‑70 (MQ=255) aCTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTAcc > 1:1620073/1‑70 (MQ=255) gTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACt < 1:2701036/71‑1 (MQ=255) ttGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTc < 1:993217/71‑1 (MQ=255) aCCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCca > 1:3105867/1‑70 (MQ=255) aCCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCca > 1:50078/1‑70 (MQ=255) cTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAg > 1:4428/1‑71 (MQ=255) cTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAg < 1:2638933/71‑1 (MQ=255) cTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAg < 1:173518/71‑1 (MQ=255) aCGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTa < 1:178006/41‑1 (MQ=255) cGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCAt < 1:561702/71‑1 (MQ=255) cTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCAcag < 1:1784506/71‑1 (MQ=255) gCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGa < 1:2925446/39‑1 (MQ=255) gCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGa < 1:2773659/39‑1 (MQ=255) gCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGa < 1:1436102/39‑1 (MQ=255) gCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGa < 1:2051809/39‑1 (MQ=255) gCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGa < 1:1621299/39‑1 (MQ=255) cAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCAcagca < 1:665313/70‑1 (MQ=255) cAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCAcagc < 1:1983193/69‑1 (MQ=255) gAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGct < 1:2406335/71‑1 (MQ=255) aaaCTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGct < 1:3135303/70‑1 (MQ=255) aaCTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGctct > 1:2182082/1‑71 (MQ=255) ggCGTAGACGTATACGCAGTATCTACCGATACTCTCTTCACCCACAAAGCATGGCACAGCAGCTCTGAAAc < 1:1951645/71‑1 (MQ=255) ggCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAAc < 1:445694/71‑1 (MQ=255) gTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAg < 1:344635/46‑1 (MQ=255) gTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCAt < 1:20965/71‑1 (MQ=255) tAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACagcagc < 1:58211/59‑1 (MQ=255) tAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACagcagc < 1:3043000/59‑1 (MQ=255) tAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACagcagc < 1:1305028/59‑1 (MQ=255) tAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACagcagc < 1:363100/59‑1 (MQ=255) gACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGctc > 1:2820148/1‑59 (MQ=255) gACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGc > 1:1528994/1‑71 (MQ=255) aCGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGc > 1:2597824/1‑70 (MQ=255) aCGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGc > 1:662188/1‑70 (MQ=255) aCGTATACGCAGTATCTACAGATACTCACTTCACCCACAAAGCATg > 1:1434447/1‑46 (MQ=255) cGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTa < 1:2594907/71‑1 (MQ=255) cGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTa < 1:41012/71‑1 (MQ=255) gTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAAc > 1:1398291/1‑62 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGcaca < 1:681711/46‑1 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGcaca < 1:409598/46‑1 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGcaca < 1:2645020/46‑1 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGcaca < 1:1484420/46‑1 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCAcag < 1:1670999/47‑1 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCAcag < 1:2104181/47‑1 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCAcag < 1:3304841/47‑1 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGa < 1:2865879/56‑1 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGa < 1:1546206/56‑1 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGa < 1:2503667/56‑1 (MQ=255) taCGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTaaaa < 1:3064258/70‑1 (MQ=255) taCGCAGCATCTACCGATACTCACTTCACCCACAAAGCATgg < 1:2051293/42‑1 (MQ=255) taCGCAGCATCTACCGATACTCACTTCACCCACAAAGCATgg < 1:728417/42‑1 (MQ=255) taCGCAGCATCTACCGATACTCACTTCACCCACAAAGCATgg < 1:2107622/42‑1 (MQ=255) cGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCAcagc > 1:1490578/1‑46 (MQ=255) cGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCAcagc > 1:2941860/1‑46 (MQ=255) cGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCAcagc > 1:3334328/1‑46 (MQ=255) cGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGaaa > 1:1986312/1‑56 (MQ=255) cGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGaaa > 1:278409/1‑56 (MQ=255) cGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGaaa > 1:684079/1‑56 (MQ=255) cGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATc > 1:245566/1‑70 (MQ=255) cGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCa < 1:2875346/71‑1 (MQ=255) cGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCa < 1:6554/71‑1 (MQ=255) cGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCa < 1:2584100/71‑1 (MQ=255) cGCAGTATCTACCGATACTCACTTCACACACAAAGCAt > 1:349283/1‑38 (MQ=255) cGCAGCATCTACCGATACTCACTTCACCCACAAAGCATGGca > 1:3423910/1‑42 (MQ=255) cGCAGCATCTACCGATACTCACTTCACCCACAAAGCATGGca > 1:1936089/1‑42 (MQ=255) gCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCaa < 1:1848852/71‑1 (MQ=255) cAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCaaa < 1:3313717/71‑1 (MQ=255) aGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTaa > 1:591640/1‑63 (MQ=255) aGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCaaa > 1:1661141/1‑70 (MQ=255) aGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCaaa > 1:1027277/1‑70 (MQ=255) tATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGctct < 1:1208900/47‑1 (MQ=255) | CCGACCGAACTGGGTGACGTTGCTGACCACTACGAAGAACTGCAGAAACTGGGCGTAGACGTATACGCAGTATCTACCGATACTCACTTCACCCACAAAGCATGGCACAGCAGCTCTGAAACCATCGCTAAAATCAAA > minE/393983‑394120 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |