| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 755,969 | C→G | 8.4% | V32V (GTC→GTG) | lolD → | outer membrane‑specific lipoprotein transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 755,969 | 0 | C | G | 8.4% | 217.0 / 8.5 | 71 | V32V (GTC→GTG) | lolD | outer membrane‑specific lipoprotein transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (22/43); new base G (4/2); total (26/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01 | |||||||||||
CTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTG > minE/755904‑756038 | cTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCg > 1:2586348/1‑70 (MQ=255)cTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:2815340/71‑1 (MQ=255) gtgCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAg > 1:3102751/1‑71 (MQ=255) tgCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAg < 1:2756266/70‑1 (MQ=255) aaCGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGaa < 1:370926/71‑1 (MQ=255) tATCAGGAAGGCTGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAatgat > 1:3398390/1‑71 (MQ=255) tATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAatgat > 1:3050139/1‑71 (MQ=255) tATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAatgat > 1:1425553/1‑71 (MQ=255) tATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAatgat > 1:2156694/1‑71 (MQ=255) aGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg < 1:1077073/71‑1 (MQ=255) aGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg < 1:2830431/71‑1 (MQ=255) aGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg < 1:2826205/71‑1 (MQ=255) ggAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGaa < 1:1394231/61‑1 (MQ=255) ggAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGaa < 1:2541339/61‑1 (MQ=255) ggAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGaa < 1:1655504/61‑1 (MQ=255) gAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGAt > 1:3357782/1‑71 (MQ=255) ggCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGAtcgt < 1:3371001/71‑1 (MQ=255) gCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGAtcgtc > 1:1743568/1‑71 (MQ=255) gCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGAtcgtc > 1:1727467/1‑71 (MQ=255) cAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGAtcgtcg > 1:579077/1‑71 (MQ=255) gtgtGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGt < 1:1413047/71‑1 (MQ=255) gtgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAg > 1:319121/1‑71 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGcta < 1:2247371/43‑3 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:2869014/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:28173/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:885506/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:1439975/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:2500986/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:1530117/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:2370257/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:396767/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:2265975/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:2250164/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:2171411/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:880336/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:1718747/43‑1 (MQ=255) tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGAtcgt < 1:3475494/64‑1 (MQ=255) gCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGa < 1:1161450/42‑1 (MQ=255) aCCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTgg > 1:1093791/1‑71 (MQ=255) gATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTc < 1:2115536/71‑1 (MQ=255) aTGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGt < 1:2376541/38‑1 (MQ=255) aTGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGt < 1:3068341/38‑1 (MQ=255) tgtgCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCg < 1:3366252/71‑1 (MQ=255) ctgcACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTaa < 1:1045661/71‑1 (MQ=255) acaATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTaa < 1:2948920/67‑1 (MQ=255) caATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTa < 1:3442883/71‑1 (MQ=255) tGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg > 1:2586803/1‑37 (MQ=255) tGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCg > 1:131510/1‑37 (MQ=255) tGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTAc < 1:2180346/69‑1 (MQ=255) tCAGTTTCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGtt < 1:668276/54‑1 (MQ=255) tCAGTTTCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGtt < 1:1379580/54‑1 (MQ=255) tCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtg > 1:2660504/1‑71 (MQ=255) tCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtg > 1:2490673/1‑71 (MQ=255) tCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtg > 1:3367882/1‑71 (MQ=255) tCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtg > 1:1805474/1‑71 (MQ=255) aGTTTCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTcc > 1:993837/1‑54 (MQ=255) aGTTTCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTcc > 1:2863685/1‑54 (MQ=255) aGTTTCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTcc > 1:410835/1‑54 (MQ=255) aGTTTCAGCGTGGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTcc > 1:466781/1‑54 (MQ=255) tttCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGc < 1:3007640/43‑1 (MQ=255) tttCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGc < 1:1199634/43‑1 (MQ=255) tttCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGc < 1:727356/43‑1 (MQ=255) tttCAGCGTCGGCGAAGGGGAAATGATGGCGATCGTCGGTAGc < 1:1638813/43‑1 (MQ=255) tCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCgg > 1:543713/1‑37 (MQ=255) tCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCgg > 1:729864/1‑37 (MQ=255) tCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGctct > 1:3284739/1‑44 (MQ=255) tCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCa < 1:2327381/70‑1 (MQ=255) tCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCa < 1:104110/70‑1 (MQ=255) aGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACct > 1:767846/1‑71 (MQ=255) tCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACctgctg < 1:717910/71‑1 (MQ=255) tCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACctgctg < 1:1928257/71‑1 (MQ=255) | CTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTG > minE/755904‑756038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |