Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 547,831:1 +G 100% pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE →
ylbE →
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb547,8311.G100.0% 74.0 / NA 23pseudogene (251/261 nt)
pseudogene (251/1259 nt)
ylbE
ylbE
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment
ECK0512:JW0508+JW0507:b4572; hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (9/14);  total (9/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTC  >  W3110S.gb/547763‑547896
                                                                     |                                                                 
gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAgg                                                                 >  1:942818/1‑71 (MQ=255)
gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAgg                                                                 <  1:1596270/71‑1 (MQ=255)
     tgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAggggg                                                              >  1:2716740/1‑68 (MQ=255)
             tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt                                                     <  1:2718048/71‑1 (MQ=255)
              gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt                                                     >  1:560492/1‑70 (MQ=255)
               tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa                                                                    <  1:2530499/54‑1 (MQ=255)
                   ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGa                                                         <  1:79409/61‑1 (MQ=255)
                   ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTcc                                               <  1:2394881/71‑1 (MQ=255)
                    tCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCt                                              >  1:2910601/1‑71 (MQ=255)
                     cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt                                             >  1:2802198/1‑71 (MQ=255)
                     cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCt                                              >  1:3154496/1‑70 (MQ=255)
                             gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt                                     >  1:388756/1‑71 (MQ=255)
                                              cAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc                    <  1:299789/71‑1 (MQ=255)
                                                ggCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa                  <  1:2830689/71‑1 (MQ=255)
                                                    cTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa                                 <  1:2821926/52‑1 (MQ=255)
                                                    cTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa                                 <  1:1780223/52‑1 (MQ=255)
                                                      ggCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa                                 >  1:1570945/1‑50 (MQ=255)
                                                       gCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc             <  1:1137994/69‑1 (MQ=255)
                                                          aTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt        <  1:3175526/71‑1 (MQ=255)
                                                             cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa                                         >  1:3008557/1‑35 (MQ=37)
                                                             cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc     <  1:443902/71‑1 (MQ=255)
                                                             cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc     >  1:1825454/1‑71 (MQ=255)
                                                              tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                  <  1:2267661/41‑1 (MQ=38)
                                                              tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                  <  1:3160523/41‑1 (MQ=38)
                                                               ggAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg                                  <  1:1988048/40‑1 (MQ=38)
                                                                gAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc     >  1:813350/1‑68 (MQ=255)
                                                                gAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc  <  1:1946262/71‑1 (MQ=255)
                                                                     |                                                                 
GTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTC  >  W3110S.gb/547763‑547896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: