Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 547,831:1 | +G | 100% | pseudogene (251/261 nt) pseudogene (251/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 547,831 | 1 | . | G | 100.0% | 74.0 / NA | 23 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (9/14); total (9/14) | |||||||||||
Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
GTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTC > W3110S.gb/547763‑547896 | gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg > 1:942818/1‑71 (MQ=255) gtgcgtgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑gg < 1:1596270/71‑1 (MQ=255) tgGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggggg > 1:2716740/1‑68 (MQ=255) tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt < 1:2718048/71‑1 (MQ=255) gTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt > 1:560492/1‑70 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCa < 1:2530499/54‑1 (MQ=255) ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGa < 1:79409/61‑1 (MQ=255) ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTcc < 1:2394881/71‑1 (MQ=255) tCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCt > 1:2910601/1‑71 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCtt > 1:2802198/1‑71 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCt > 1:3154496/1‑70 (MQ=255) gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt > 1:388756/1‑71 (MQ=255) cAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGcc < 1:299789/71‑1 (MQ=255) ggCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa < 1:2830689/71‑1 (MQ=255) cTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa < 1:2821926/52‑1 (MQ=255) cTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa < 1:1780223/52‑1 (MQ=255) ggCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGa > 1:1570945/1‑50 (MQ=255) gCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGc < 1:1137994/69‑1 (MQ=255) aTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTAt < 1:3175526/71‑1 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCa > 1:3008557/1‑35 (MQ=37) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc < 1:443902/71‑1 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc > 1:1825454/1‑71 (MQ=255) tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:2267661/41‑1 (MQ=38) tGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:3160523/41‑1 (MQ=38) ggAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:1988048/40‑1 (MQ=38) gAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc > 1:813350/1‑68 (MQ=255) gAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTc < 1:1946262/71‑1 (MQ=255) | GTGCGTGGGCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTTC > W3110S.gb/547763‑547896 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |