| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 155,463 | A→T | 100% | *247K (TAA→AAA) | ecpD ← | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 155,463 | 0 | A | T | 100.0% | 194.2 / NA | 66 | *247K (TAA→AAA) | ecpD | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (40/26); total (40/26) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAG > W3110S.gb/155396‑155528 | gatgatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg > 1:113510/1‑71 (MQ=255)gatgatAATTTTTAGTATATTATATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAg > 1:1407543/1‑71 (MQ=255) gatAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCt > 1:1662256/1‑71 (MQ=255) tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg > 1:2484166/1‑71 (MQ=255) tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg < 1:2681208/71‑1 (MQ=255) tAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTgg < 1:2285239/71‑1 (MQ=255) aTTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGc < 1:383301/70‑1 (MQ=255) tttttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt > 1:754657/1‑71 (MQ=255) ttttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt < 1:2466945/70‑1 (MQ=255) tttAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTAATTTCAGCCTGGCATTa > 1:2198952/1‑71 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc > 1:2164432/1‑65 (MQ=255) tatTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:3223359/1‑71 (MQ=255) atTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGc < 1:1047395/54‑1 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGc < 1:457974/58‑1 (MQ=255) ttCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt > 1:889147/1‑71 (MQ=255) tCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCAt > 1:52037/1‑59 (MQ=255) cTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgc > 1:979235/1‑71 (MQ=255) ataGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTa > 1:1279918/1‑58 (MQ=255) tattCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc < 1:2160328/67‑1 (MQ=255) taGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc > 1:3702430/1‑70 (MQ=255) taGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc > 1:1280951/1‑70 (MQ=255) taGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcgc > 1:556136/1‑70 (MQ=255) cACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacc > 1:3119784/1‑70 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTAc > 1:2058158/1‑53 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt > 1:1113846/1‑61 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca < 1:149377/70‑1 (MQ=255) aCGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccacca > 1:1304668/1‑70 (MQ=255) cGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc < 1:294320/56‑1 (MQ=255) aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa > 1:1438896/1‑71 (MQ=255) aTGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa > 1:2608245/1‑71 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc > 1:574665/1‑38 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTc < 1:1227446/69‑1 (MQ=255) tGCTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt > 1:1248037/1‑71 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGcc > 1:764634/1‑36 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:1516386/70‑1 (MQ=255) cTTCCTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:375263/70‑1 (MQ=255) ccTGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa < 1:3230120/69‑1 (MQ=255) cTGCGGAATTATATACTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATg < 1:324933/70‑1 (MQ=255) tGCGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGt < 1:2175396/61‑1 (MQ=255) cGGAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAAt > 1:2866721/1‑66 (MQ=255) ggAATTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGt > 1:479411/1‑70 (MQ=255) aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTc < 1:3284562/39‑1 (MQ=255) aaTTATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcg < 1:2349299/46‑1 (MQ=255) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:1227714/1‑39 (MQ=255) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCaa > 1:1802839/1‑39 (MQ=255) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTgcg < 1:1745718/44‑1 (MQ=255) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAtt < 1:1933432/58‑1 (MQ=255) ttATATCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTa < 1:354615/59‑1 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAAt > 1:95887/1‑38 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCaaa > 1:998877/1‑50 (MQ=255) atatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATg > 1:1216383/1‑60 (MQ=255) tatCCTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATg < 1:2158967/70‑1 (MQ=255) ccTTGCCTGATTTCAGCCTGGCATTACCTTCAAtt < 1:2168344/35‑1 (MQ=255) ccTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGccac > 1:3481817/1‑42 (MQ=255) cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTaa < 1:1777101/53‑1 (MQ=255) cTTGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACtt < 1:125142/71‑1 (MQ=255) ttGCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACttt > 1:2597056/1‑71 (MQ=255) gCCTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg > 1:1473062/1‑71 (MQ=255) ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt > 1:946940/1‑46 (MQ=255) ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCAt > 1:3609250/1‑46 (MQ=255) ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg < 1:3205302/70‑1 (MQ=255) ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTg > 1:1267134/1‑70 (MQ=255) ccTGATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGc < 1:2987392/71‑1 (MQ=255) gATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAg > 1:3404185/1‑70 (MQ=255) gATTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAg > 1:124335/1‑70 (MQ=255) aTTTCAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCa > 1:81158/1‑41 (MQ=255) | GATGATAATTTTTAGTATATTCTATAGTCACGCTATGCTTCCTGCGGAATTATATCCTTGCCTGATTACAGCCTGGCATTACCTTCAATTGCGCCACCAAAGTCATTAATGGCGTAAAAATGCACTTTTGCAG > W3110S.gb/155396‑155528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |