| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 454,926 | C→T | 9.7% | G190G (GGC→GGT) | tig → | peptidyl‑prolyl cis/trans isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 454,926 | 0 | C | T | 9.7% | 114.5 / 2.1 | 41 | G190G (GGC→GGT) | tig | peptidyl‑prolyl cis/trans isomerase |
| Reads supporting (aligned to +/- strand): ref base C (14/23); new base T (2/2); total (16/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01 | |||||||||||
CTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATC > W3110S.gb/454857‑454995 | cTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGcc < 1:1455300/71‑1 (MQ=255) cACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCa < 1:1302839/69‑1 (MQ=255) ggTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCg < 1:3027453/71‑1 (MQ=255) ggTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCg < 1:1540825/71‑1 (MQ=255) ttCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCATCTGATTTCGTACTGGCGATGGGCCAGGGTCGTa > 1:3269932/1‑71 (MQ=255) tCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAg > 1:2709148/1‑63 (MQ=255) cTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTAt < 1:3149465/70‑1 (MQ=255) cTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTAt > 1:3332298/1‑70 (MQ=255) cTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTAt < 1:1503854/70‑1 (MQ=255) tAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGc < 1:2023710/56‑1 (MQ=255) gACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGTCAGGGTCGTATGCTcc > 1:3703470/1‑70 (MQ=255) gACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGTCAGGGTCGTATGATccc > 1:2835870/1‑71 (MQ=255) gACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTAt > 1:2153593/1‑65 (MQ=255) gACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATccc < 1:1469700/71‑1 (MQ=255) aCGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCTATGGGTCAGGGTCGTATGATCCCg < 1:561653/71‑1 (MQ=255) aCGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGTCAGGGTCGTATGATCCCg < 1:3469991/71‑1 (MQ=255) aaGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCttt > 1:3037574/1‑71 (MQ=255) agagTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTg < 1:3565122/71‑1 (MQ=255) gagTTCGAAGGCGGTAAAGCGTCTGATTTGGTACTGGCGATGGGCCAGGGTCg < 1:139026/53‑1 (MQ=255) agTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCg < 1:884931/52‑1 (MQ=255) gTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGaa < 1:403566/70‑1 (MQ=255) ttCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGcc < 1:3358033/43‑1 (MQ=255) ttCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGcc < 1:1202051/43‑1 (MQ=255) cGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACg > 1:843522/1‑71 (MQ=255) gTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCaa < 1:1037026/70‑1 (MQ=255) aaGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGc > 1:2654491/1‑71 (MQ=255) cGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCcac < 1:1724349/71‑1 (MQ=255) gTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCgg > 1:1878043/1‑44 (MQ=255) gTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCcaca < 1:1080338/71‑1 (MQ=255) cTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCaaa > 1:1626884/1‑62 (MQ=255) aTTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTg < 1:2585269/45‑1 (MQ=255) aTTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAg > 1:1766813/1‑60 (MQ=255) aTTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCt < 1:2258332/71‑1 (MQ=255) tttCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACgg < 1:3572725/51‑1 (MQ=255) tCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGc > 1:2852663/1‑71 (MQ=255) gTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCg < 1:2585238/70‑1 (MQ=255) tGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTg < 1:1111366/36‑1 (MQ=255) tGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATc < 1:3543929/47‑1 (MQ=255) tGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACaaa < 1:1998321/59‑1 (MQ=255) gCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAag > 1:1735077/1‑67 (MQ=255) gCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGt > 1:2004207/1‑70 (MQ=255) cGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTc < 1:3483577/71‑1 (MQ=255) ggCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCaaa > 1:1644084/1‑42 (MQ=255) gCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACaaa < 1:36900/50‑1 (MQ=255) gCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATc < 1:1437612/71‑1 (MQ=255) | CTTCACCGGTTCTGTAGACGGCGAAGAGTTCGAAGGCGGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATC > W3110S.gb/454857‑454995 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |