| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,694 | A→G | 100% | pseudogene (114/261 nt) pseudogene (114/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,694 | 0 | A | G | 100.0% | 102.2 / NA | 32 | pseudogene (114/261 nt) pseudogene (114/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (17/15); total (17/15) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAA > W3110S.gb/547627‑547757 | ttCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAggg > 1:3405971/1‑70 (MQ=255) gtcgCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCaa < 1:67023/70‑1 (MQ=255) gTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCtt < 1:3553472/71‑1 (MQ=255) acacTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACg > 1:2380915/1‑71 (MQ=255) ctggctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCg > 1:1771438/1‑71 (MQ=255) gctggATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgc > 1:132422/1‑71 (MQ=255) tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATg > 1:1834256/1‑71 (MQ=255) tgCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATg > 1:1829828/1‑71 (MQ=255) aaCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:2982699/64‑1 (MQ=255) aaCCGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgcc < 1:1383268/64‑1 (MQ=255) cGGCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTgg < 1:2960781/71‑1 (MQ=255) gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGcc < 1:3276873/51‑1 (MQ=255) gCTTCTTCACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGcc < 1:3165055/51‑1 (MQ=255) tcttcACTTATCAGCGAACTAAACGCGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCa < 1:686337/68‑1 (MQ=255) tcttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACg < 1:3594790/46‑1 (MQ=255) ttcACTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCgg > 1:312343/1‑48 (MQ=255) aCTTATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGa > 1:2802310/1‑71 (MQ=255) tataAGCGAACTAAACGAGGGCAAAACACTGCTTCACGc > 1:319614/1‑39 (MQ=37) tATCAGGGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCa > 1:474227/1‑49 (MQ=38) tATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa > 1:3574488/1‑50 (MQ=255) tATCAGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa > 1:3578586/1‑50 (MQ=255) tCAGCGAACTAAACGAGGGCAAAACACTGCTTCAc < 1:2382507/35‑1 (MQ=255) aGCGAACTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGAcc > 1:3581142/1‑66 (MQ=255) aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa < 1:328108/42‑1 (MQ=255) aaCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCa < 1:1732050/52‑1 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGgccgc < 1:3105598/38‑1 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGgccg > 1:1156288/1‑37 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGgccg > 1:1597713/1‑37 (MQ=255) aCTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATg > 1:138394/1‑70 (MQ=255) cTAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaa < 1:2072023/71‑1 (MQ=255) tAAACGAGGGCAAAACACTGCTTCACGCCGGGCCGCCaa > 1:1575127/1‑39 (MQ=255) aaCGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaaa < 1:3054590/69‑1 (MQ=255) cGAGGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGaaa > 1:228827/1‑67 (MQ=255) | TTCGTCGCGCTCGTCCACACTGGCTGGATGTGCAACCGGCTTCTTCACTTATCAGCGAACTAAACGAAGGCAAAACACTGCTTCACGCCGGGCCGCCAATGCGCTGGCAGGAGATGACCGGACCCATGAAA > W3110S.gb/547627‑547757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |