| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 70,581 | C→T | 93.9% | V65V (GTC→GTT) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 70,581 | 0 | C | T | 93.9% | 1077.7 / 45.3 | 390 | V65V (GTC→GTT) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base C (13/11); new base T (175/191); total (188/202) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
Only 100 of 428 total aligned reads displayed.
AAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGG > W3110S.gb/70513‑70648 | aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg < 1:3250280/71‑1 (MQ=255)aaaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:3310684/1‑71 (MQ=255) aaGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGc > 1:743101/1‑71 (MQ=255) tataTTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc > 1:1117551/1‑71 (MQ=255) tataTTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc > 1:1225195/1‑71 (MQ=255) tataTTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc > 1:1750740/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCg > 1:2244067/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCg > 1:949444/1‑71 (MQ=255) atatTCTCAATCTCACCATTCGCGGTCAGGGCGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGAcc < 1:3042752/70‑1 (MQ=255) atTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCg > 1:839374/1‑69 (MQ=255) ttCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGt > 1:1553835/1‑71 (MQ=255) tctcAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGt > 1:71279/1‑70 (MQ=255) cAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGata > 1:1955114/1‑71 (MQ=255) aaTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat > 1:1369315/1‑71 (MQ=255) aaTCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGat < 1:3448558/69‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAAtttgtttg > 1:35486/1‑50 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtttt < 1:3381510/68‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGc < 1:1819853/70‑1 (MQ=255) aCCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGGGATAttt < 1:2874109/67‑1 (MQ=255) ccATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtttt > 1:2440000/1‑67 (MQ=255) cATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGatat < 1:3282225/63‑1 (MQ=255) cATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGt < 1:3323355/71‑1 (MQ=255) cATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGt < 1:1159810/71‑1 (MQ=255) aTTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATAtt < 1:1349258/63‑1 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGt > 1:2338156/1‑69 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGt > 1:317790/1‑69 (MQ=255) ttCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGAAATTTTGCTGTTc > 1:162493/1‑71 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGCCCGGGTGATATTTTGCTGTTcc > 1:2022725/1‑70 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTccc > 1:1278997/1‑71 (MQ=255) cgcgGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTccc > 1:1975398/1‑71 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:399736/71‑1 (MQ=255) ggTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:1246605/71‑1 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc > 1:1499339/1‑70 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:3157357/70‑1 (MQ=255) gTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:1838346/70‑1 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg < 1:1442770/58‑1 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTg < 1:3654124/58‑1 (MQ=255) tCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:2890744/71‑1 (MQ=255) cAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCggg > 1:2809066/1‑47 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:432062/1‑69 (MQ=255) aGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCggg > 1:2202562/1‑46 (MQ=255) gggggTGGTGAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga < 1:1013828/70‑1 (MQ=255) ggggTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGa < 1:96589/47‑1 (MQ=255) tggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGAtt > 1:584211/1‑71 (MQ=255) ggtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:1775247/60‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGAcc < 1:3088954/35‑1 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca > 1:2971215/1‑71 (MQ=255) gtgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtca < 1:1053143/71‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGt < 1:1311015/38‑1 (MQ=255) tgAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgagaga < 1:1095735/66‑1 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat > 1:2670480/1‑70 (MQ=255) gAAAAATCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:3345555/70‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGata > 1:2006932/1‑40 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGat < 1:2313652/39‑1 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCt > 1:912819/1‑47 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:3587643/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:3694823/1‑71 (MQ=255) aaaaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatc < 1:1648947/70‑1 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTcc > 1:3622618/1‑50 (MQ=255) aaaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:859660/1‑70 (MQ=255) aaaTCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc > 1:1916098/1‑70 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:2131667/53‑1 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAg > 1:2756361/1‑55 (MQ=255) aaTCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTa < 1:3568391/71‑1 (MQ=255) tCAGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACg < 1:2941325/71‑1 (MQ=255) tCAGGGACGAGAATTTGTCTGCCGACCGGGTGATAtttt < 1:2381320/39‑1 (MQ=255) cAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGtt < 1:1397830/44‑1 (MQ=255) cAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTGCCGCCAGga < 1:3136628/54‑1 (MQ=255) aGGGACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACgg < 1:1603111/70‑1 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgtcg < 1:789609/71‑1 (MQ=255) ggACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGgt < 1:2615743/69‑1 (MQ=255) gACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:1682242/52‑1 (MQ=255) gACGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga > 1:3477864/1‑50 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcat < 1:1791343/57‑1 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCa < 1:3221422/71‑1 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCa > 1:3000447/1‑71 (MQ=255) cGAGAATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCa > 1:2498643/1‑71 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga < 1:2656855/47‑1 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga > 1:885159/1‑47 (MQ=255) gagaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCAt > 1:259585/1‑71 (MQ=255) agaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:815407/42‑1 (MQ=255) agaATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGc < 1:2849344/41‑1 (MQ=255) gaATTTGTTTGCCGCCCGGGTGATATTTTGCTGTTACCGCCAGGAGAGATTCATCACTACGGTCGTCATcc > 1:5645/1‑71 (MQ=255) gaATTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTTGTCATcc > 1:2702500/1‑71 (MQ=255) aaTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGcc < 1:2057964/40‑1 (MQ=255) aaTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAgg < 1:620244/43‑1 (MQ=255) aaTTTGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCAc < 1:3497231/56‑1 (MQ=255) aaTTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGgaga < 1:1574101/46‑1 (MQ=255) tttgtttgTCGACCGGGGGATATTTTGCGGTTCCCGCCAGgagaga < 1:2100019/46‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAg < 1:539351/40‑1 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATtcatca > 1:2274232/1‑53 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:461102/1‑69 (MQ=255) tttgtttgCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCg > 1:1703563/1‑69 (MQ=255) ttGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTa > 1:1434040/1‑55 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGga > 1:2400780/1‑40 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACgg > 1:2522558/1‑57 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAgg > 1:271657/1‑71 (MQ=255) tGTTTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAg < 1:221857/70‑1 (MQ=255) tGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAgg > 1:1877953/1‑71 (MQ=255) tGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAg > 1:499694/1‑70 (MQ=255) | AAAGGTTATATTCTCAATCTCACCATTCGCGGTCAGGGGGTGGTGAAAAATCAGGGACGAGAATTTGTCTGCCGACCGGGTGATATTTTGCTGTTCCCGCCAGGAGAGATTCATCACTACGGTCGTCATCCGGAGG > W3110S.gb/70513‑70648 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |