| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,303,633 | G→T | 100% | Q246Y (CAG→TAT) | oppA → | oligopeptide transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,303,633 | 0 | G | T | 100.0% | 92.5 / NA | 33 | Q246Y (CAG→TAT) | oppA | oligopeptide transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (14/19); total (14/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT > W3110S.gb/1303568‑1303695 | aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:3555036/1‑71 (MQ=255)aacgaacgAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAAc > 1:3390025/1‑71 (MQ=255) aacgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt < 1:3300721/71‑1 (MQ=255) acgaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt > 1:3600122/1‑71 (MQ=255) gaaTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTg < 1:3230095/71‑1 (MQ=255) gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAtt > 1:2614160/1‑64 (MQ=255) gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTAt > 1:2131145/1‑57 (MQ=255) gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCtt < 1:1398109/70‑1 (MQ=255) cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:1672820/71‑1 (MQ=255) cGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa < 1:3070256/71‑1 (MQ=255) cAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc > 1:3136347/1‑57 (MQ=255) cAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg > 1:1050164/1‑71 (MQ=255) aGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt < 1:3694060/59‑1 (MQ=255) cGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:1041571/71‑1 (MQ=255) aCCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCt < 1:1984657/60‑1 (MQ=255) ccTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGAt < 1:3488036/71‑1 (MQ=255) cTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg < 1:2041978/71‑1 (MQ=255) tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGt > 1:2070904/1‑71 (MQ=255) cTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGc > 1:3077632/1‑44 (MQ=38) tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:221327/44‑1 (MQ=38) tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc < 1:3379021/63‑1 (MQ=255) ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTAcc > 1:1652514/1‑62 (MQ=255) gAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTg < 1:2439211/53‑1 (MQ=255) caacGCGAAAACCGTTATTAACTATGTAACCTATTTGcc < 1:2441662/39‑1 (MQ=37) acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTTAAGTTACCGATGTCAACCGCTAc > 1:2885510/1‑71 (MQ=255) acGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATg < 1:1711990/60‑1 (MQ=255) gcgAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCg < 1:1088366/71‑1 (MQ=255) aaaaCCTTTATTAACTATGTAACCTATTTGCCTATTGCTTc < 1:2035989/41‑1 (MQ=37) aaaCCGTTATTAACTATGTAACATATTTGCCTATTGCTTCTGAAGTTACCGATGTCa < 1:1896963/57‑1 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggtg > 1:2866862/1‑67 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaaa < 1:1462169/70‑1 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGa > 1:3471099/1‑68 (MQ=255) attaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAt > 1:3341098/1‑71 (MQ=255) | AACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAAT > W3110S.gb/1303568‑1303695 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |