| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,265,554 | T→C | 7.4% | P110P (CCA→CCG) | tdcB ← | catabolic threonine dehydratase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,265,554 | 0 | T | C | 7.4% | 141.5 / 3.4 | 54 | P110P (CCA→CCG) | tdcB | catabolic threonine dehydratase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (17/33); new base C (2/2); total (19/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.07e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTG > W3110S.gb/3265495‑3265622 | ttATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCttt < 1:913307/71‑1 (MQ=255) tATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTCGGCGCACCtttt > 1:3524609/1‑71 (MQ=255) aTCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTg > 1:2437877/1‑59 (MQ=255) aTCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTg > 1:3002016/1‑71 (MQ=255) tCACCATGCAGAACGACTTCTGCGGAGTAGTCGCAGGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTgg < 1:1472159/71‑1 (MQ=255) tGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATcac < 1:2956812/71‑1 (MQ=255) gCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCAcc > 1:1486848/1‑58 (MQ=255) cAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATcacca < 1:1631435/71‑1 (MQ=255) gAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCAc < 1:2239338/54‑1 (MQ=255) gAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATcac < 1:814540/67‑1 (MQ=255) aCGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACttt < 1:1585581/71‑1 (MQ=255) aCGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACtt > 1:2310464/1‑70 (MQ=255) aCTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTa > 1:2066432/1‑69 (MQ=255) cTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCtt < 1:1470028/51‑1 (MQ=255) cTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACtt < 1:2208733/66‑1 (MQ=255) ttCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATcaccac < 1:294356/63‑1 (MQ=255) ttCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGt > 1:2398106/1‑71 (MQ=255) cTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTc > 1:41749/1‑70 (MQ=255) cTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTc > 1:1488741/1‑70 (MQ=255) agtagtCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAcc > 1:3382432/1‑70 (MQ=255) tagtCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTAc < 1:303071/58‑1 (MQ=255) tagtCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAg < 1:971796/71‑1 (MQ=255) gtCGCACGTTGCCGCTACTTTGGATTTTGGCGCAcc > 1:3056745/1‑36 (MQ=255) gtCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCttt < 1:3711142/39‑1 (MQ=255) gtCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGc < 1:1306718/43‑1 (MQ=255) cGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACt > 1:2810834/1‑50 (MQ=255) cGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACGCAGCATc > 1:3271425/1‑71 (MQ=255) agCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACt > 1:573733/2‑50 (MQ=255) aCGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATcgcg < 1:1212495/71‑1 (MQ=255) gTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTc < 1:1689214/53‑1 (MQ=255) ttGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCa < 1:3051061/63‑1 (MQ=255) ttGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCAATACCCAGCATCGCGCa < 1:3202345/70‑1 (MQ=255) ccGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAgta < 1:980921/70‑3 (MQ=255) ccGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGgag < 1:2618763/71‑1 (MQ=255) ccGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGga < 1:3704655/70‑1 (MQ=255) cGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGga < 1:2873570/69‑1 (MQ=255) cGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGga < 1:1352040/69‑1 (MQ=255) gCTACTTTGGATTTTGGCGCACCTTTTGGCATcac < 1:718183/35‑1 (MQ=255) gCTACTTTGGATTTTGGCGCACCTTTTGGCATcac < 1:220614/35‑1 (MQ=255) gCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGgag < 1:197230/69‑1 (MQ=255) cTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATa > 1:2131527/1‑50 (MQ=255) cTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATcgcg < 1:2447126/62‑1 (MQ=255) cTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAg > 1:1938654/1‑65 (MQ=255) tACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAgg > 1:1330786/1‑70 (MQ=255) aCTTTGGATTTCGGCGCACCTTTTGGCATCACCACTTTACCGTCAATACCCAGCATcgc > 1:301626/1‑59 (MQ=255) tttGGATTTTGGCGCACCTTTTGGCATCACCACttt < 1:1872728/36‑1 (MQ=255) tttGGATTTTGGCGCACCTTTTGGCATCACCACttt < 1:2794106/36‑1 (MQ=255) tttGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGagag < 1:822109/66‑1 (MQ=255) tttGGATTTCGGCGCACCTTTTGGCATCACCACTTTACCGTCAATACCCAGCATCgcgc < 1:2195699/59‑1 (MQ=255) tttGGATTTCGGCGCACCTTTTGGCATCACCACTTTACCGTCAATACCCAGCATCgcgc < 1:748806/59‑1 (MQ=255) tGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATAc > 1:922032/1‑45 (MQ=255) gATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAAcccc < 1:3397013/71‑1 (MQ=255) aTTTTGGCGCACCTTTTGGCATCACCACTTTACCGTc < 1:3032357/37‑1 (MQ=255) aTTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGga < 1:1309050/58‑1 (MQ=255) tttGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTg > 1:1397739/1‑71 (MQ=255) | TTATCACCATGCAGAACGACTTCTGCGGAGTAGTCGCACGTTGCCGCTACTTTGGATTTTGGCGCACCTTTTGGCATCACCACTTTACCGTCGATACCCAGCATCGCGCAGGAGAGGGAAACCCCTTG > W3110S.gb/3265495‑3265622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |