| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,388,193 | A→T | 5.7% | V263V (GTT→GTA) | aaeA ← | p‑hydroxybenzoic acid efflux system component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,388,193 | 0 | A | T | 5.7% | 189.0 / 3.7 | 70 | V263V (GTT→GTA) | aaeA | p‑hydroxybenzoic acid efflux system component |
| Reads supporting (aligned to +/- strand): ref base A (28/38); new base T (2/2); total (30/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01 | |||||||||||
GTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGT > W3110S.gb/3388125‑3388260 | gTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAAc < 1:1773690/71‑1 (MQ=255) accaccACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCgg < 1:3029374/71‑1 (MQ=255) caccacTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGaa < 1:514913/71‑1 (MQ=255) ccacCGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGTACCGGAaca > 1:1006491/1‑71 (MQ=255) ccacCGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGTACCGGAaca > 1:1907122/1‑71 (MQ=255) cTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGt > 1:2908920/1‑71 (MQ=255) cTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGt > 1:1919530/1‑71 (MQ=255) gTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGaa > 1:2605285/1‑58 (MQ=255) tAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTgc > 1:1010467/1‑71 (MQ=255) tAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTgc > 1:1563330/1‑71 (MQ=255) tAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTgc > 1:2375911/1‑71 (MQ=255) gCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCgg < 1:1740763/59‑1 (MQ=255) gCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTgcg > 1:2567184/1‑70 (MQ=255) gtggtgCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAg < 1:2381156/71‑1 (MQ=255) tggtgCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCaa < 1:994808/69‑1 (MQ=255) tggtgCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGa > 1:1518165/1‑71 (MQ=255) gtgCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCaa > 1:784979/1‑67 (MQ=255) gtgCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACg > 1:2673683/1‑71 (MQ=255) cccGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCAc > 1:3030396/1‑71 (MQ=255) cAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCAt < 1:499631/71‑1 (MQ=255) ggCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTc < 1:1126141/71‑1 (MQ=255) ccAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCgg < 1:1668095/42‑1 (MQ=255) ccAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCgg < 1:3268810/42‑1 (MQ=255) ccAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGTAACACga > 1:2937291/1‑48 (MQ=255) cAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCa < 1:2400354/69‑1 (MQ=255) cAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAg > 1:3303638/1‑71 (MQ=255) gATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCgg < 1:1577632/39‑1 (MQ=255) aTGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCaa < 1:2292536/52‑1 (MQ=255) aTGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAg < 1:1695024/53‑1 (MQ=255) aTGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAg < 1:887654/53‑1 (MQ=255) tGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTa < 1:645270/71‑1 (MQ=255) tGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTa < 1:751834/71‑1 (MQ=255) tGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTa < 1:1884477/71‑1 (MQ=255) ttCTCTTGCTGGTTGTCGAGACGAATACGAACCgg < 1:633144/35‑1 (MQ=255) ctctTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTagag < 1:553989/70‑1 (MQ=255) ctctTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGt > 1:1871337/1‑71 (MQ=255) ctctTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGt > 1:2668447/1‑71 (MQ=255) ctTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTc > 1:1762824/1‑70 (MQ=255) ctTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTc > 1:1300931/1‑70 (MQ=255) ttGCTGGTTGTCGAGACGAATACGAACCGGAACACGt > 1:635937/1‑37 (MQ=255) ttGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTgcgc < 1:3392593/42‑1 (MQ=255) ttGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGc > 1:2434689/1‑49 (MQ=255) ttGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGt < 1:15849/68‑1 (MQ=255) ttGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTc < 1:777783/69‑1 (MQ=255) gCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCtat < 1:1555068/70‑1 (MQ=255) gCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCta < 1:2763482/69‑1 (MQ=255) gCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCt < 1:888337/68‑1 (MQ=255) ggTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTaga > 1:2600784/1‑61 (MQ=255) gTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTc < 1:2635685/63‑1 (MQ=255) gTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCg > 1:37315/1‑71 (MQ=255) ttGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTc < 1:2471863/62‑1 (MQ=255) ttGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGc < 1:427819/71‑1 (MQ=255) tGTCGAGACGAATACGAACCGGAACACGTTGCGCaa > 1:291337/1‑36 (MQ=255) tCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCAt > 1:1840850/1‑71 (MQ=255) cGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATc > 1:437481/1‑71 (MQ=255) gaggCGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTc < 1:615935/45‑1 (MQ=255) gaCGAATACGAACCGGAACACGTTGCGCAAGACGCACCCAt > 1:1088660/1‑41 (MQ=255) gaCGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCt < 1:1711613/71‑1 (MQ=255) aCGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTc < 1:2718631/42‑1 (MQ=255) cGAATACGTACCGGAACACGTTGCGCCAAACGTACCCATTCAAGGTTAGAGTCGATGGTCGCCATCCCttt < 1:1077840/71‑1 (MQ=255) cGAATACGTACCGGAACACGTTGCGCCAAACGTACCCATTCAAGGTTAGAGTCGATGGTCGCCATCCCttt < 1:1240016/71‑1 (MQ=255) gAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTg > 1:89808/1‑71 (MQ=255) gAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAG‑GTCTATAGTCGCCATCCCTTTgt > 1:72811/1‑71 (MQ=255) tACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGcc < 1:3459511/59‑1 (MQ=255) tACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGcc < 1:2605079/59‑1 (MQ=255) tACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGc < 1:2771512/58‑1 (MQ=255) aCGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtcg < 1:3380666/70‑1 (MQ=255) cGAACCGGAACACGTTGCGCAAGACGCCCCCATTCaa > 1:947986/1‑37 (MQ=255) cGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTa > 1:765237/1‑42 (MQ=255) cGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtcgt < 1:2523069/70‑1 (MQ=255) cGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTgtcgt < 1:3067415/70‑1 (MQ=255) | GTGACCACCACTGTAGCAGTGGTGCCCGCAGGCCAGATGTTCTCTTGCTGGTTGTCGAGACGAATACGAACCGGAACACGTTGCGCAAGACGCACCCATTCAAGGTTAGAGTCTATAGTCGCCATCCCTTTGTCGT > W3110S.gb/3388125‑3388260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |